IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

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  1. Method Development and Validation of Clopidogrel Bisulphate by Reverse Phase-HPLC in Bulk and Pharmaceutical Dosage FormsDownload Article

    A.Mounika, N.Sriram
    • Article Type: Research Article
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    • Pages (1-7)
    • No of Download = 1058

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    A new, simple sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Clopidogrel bisulphate was chromatographed on a reverse phase C18column (150 mm x 4.5 mm, i.d 5μm) in a mobile phase consisting of acetonitrile and phosphate buffer (pH: 3.0) in the ratio of 60:40 % v/v. The mobile phase was pumped at a flow rate of 1 ml/min with detection at 224 nm. The detector response was linear in the concentration of 50-150 μg /ml. The limit of detection and limit of quantitation was found to be 1.3 and 4.2 µg/ml, respectively. The intra and inter day variation was found to be less than 2%. The mean recovery of the drug from the solution was 99.79%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Key words: Clopidogrel bisulphate, RP-HPLC, Validation, Accuracy, Precision.

  2. Studies on Anti-HIV activity and Cytotoxicity of Wrightia tomentosa LeafDownload Article

    Periyasamy Selvam, E De Clercq
    • Article Type: Research Article
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    • Pages (8-11)
    • No of Download = 478

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    Wrightia tomentosa leaf extracts and its isolated compounds (isatin, indirubin and indigotin) have been studied for inhibition of HIV-1 and -2 replication in MT-4 cells. Cytotoxicity was also investigated in uninfected MT-4 cells (C-type Adult T Leukemia cells). All the extracts and isolated compounds exhibited cytotoxicity in MT-4 cells (CC50: 8-117 μg/ml) and Indirubin displayed marked cytostatic properties in MT-4 cells (CC50:8.32±3.83 μg/ ml).

  3. RP-HPLC Assay Method Validation for the estimation of new Anti-retroviral drug Lamivudine in Bulk and Tablet dosage formDownload Article

    M.Venkatesh, M.Bhaskar.
    • Article Type: Research Article
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    • Pages (12-17)
    • No of Download = 557

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    A Reverse phase HPLC method was developed for estimation of the Lamivudine in bulk and tablet formulation by using ODS column (250mm×4.6mm, 5µm) and Acetate buffer: acetonitrile (50:50) as mobile phase, at a flow rate of 1.5ml/min. The detection was carried at the 272nm the retention time of the Lamivudine is 1.850. The developed method was validated for the various parameters as per the ICH guidelines like accuracy precision, linearity and range, Robustnes. Linearity was obtained in the concentration range of 10µg/ml to 50µg/ml with correlation coefficient of 0.999. The accuracy of the method was assessed by recovery studies at three different concentration levels. The percentage recovery of Lamivudine was found to be in the range of 98% -102%. The method was found to be precise as indicated by the repeatability, inter-day, intra-day analysis, showing %RSD less than 2. Key words: RP-HPLC, Lamivudine, Pharmaceutical dosage form.

  4. Synthesis, Anti-Hiv activity and Cytotoxicity of 2-Phenyl, 3-Sulphonamido Quinazolin-4 (3h) -onesDownload Article

    P.Selvam, C. Pannecouque, E. De Clercq
    • Article Type: Research Article
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    • Pages (18-22)
    • No of Download = 605

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    A series of novel 2,3-disubstitutedquinazolin-4(3H)-ones have been synthesized by condensation of 2-substituted benzo [1,3] oxazine-4-ones and primary amines. Their chemical structures were assigned by means of spectral analysis (FT-IR and 1H-NMR). Synthesized compounds were screened for in vitro antiviral activity against HIV-1 and -2 replication in MT-4 cells. Cytotoxicity was also investigated in uninfected MT-4 cells (C-type Adult T Leukemia cells). All the synthesized compounds exhibited cytotoxicity in MT-4 cells (CC50: 1.93-87 μg/ml) and compound SPB-III displayed marked cytostatic properties in MT-4 cells (CC50:1.93± 0.29μg/ ml).

  5. Method Development and Validation on Etomidate injection by RP-HPLCDownload Article

    Kalpana Gopagani, M.Bhaskar
    • Article Type: Research Article
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    • Pages (23-28)
    • No of Download = 1314

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    A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.

  6. Method Development and Validation of Naftopidil by Reverse Phase-HPLC in Bulk and Pharmaceutical Dosage FormsDownload Article

    V.Gayathri, M.Bhaskar
    • Article Type: Research Article
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    • Pages (29-34)
    • No of Download = 1090

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    A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.

  7. In-vivo analgesic, anti-inflammatory and central nervous system - locomotor activities of certain synthesized 2-[(1-((phenyl amino) methyl) substituted 1H-benzoimidazol-2-yl) alkyl] isoindoline-1, 3-dionesDownload Article

    S. Selvakumar, P.C.Agarawal, I. Sudheer Babu
    • Article Type: Research Article
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    • Pages (35-47)
    • No of Download = 198

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    Two homologous series of 1, 2-disubstituted benzimidazoles carrying isoindolines (6a-n) were synthesized by mannich type reaction of 2-alkyl benzimidazolyl isoindoline-1,3-dione (4a-b) with different substituted aromatic primary amines (5a-g) using formaldehyde in acid as a condensing agent. The pthalic anhydride (1) and amino acids -glycine, alanine (2a-b) fused at 180° c to give 2-(1, 3-dioxoisoindolin-2-yl) carboxylic acids (3a-b). These acids undergo cyclization with 1, 2 diaminobenzene yield (4a-b). The structures of the synthesized isoindolines were confirmed by spectral analysis. The synthesized benzimidazolyl isoindoline were screened for their in-vivo antinociceptive activities. Most of the synthesized isoindolines exhibited significant analgesic and anti-inflammatory activities. Among this isoindolines 6c, 6d, 6e, 6f, 6l, 6k, 6m, 6n were showed appreciable antinociceptive potency. The isoindolines (4a-b) and (6a-n) were screened for their in-vivo central nervous system locomotor activities. Among these tested isoindolines 4a, 4b, 6a, 6b, 6e, 6f were only shows elevated central nervous system depressant potency.

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