IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

Search form

Articles

  1. Formulation and invitro Characterisation of Mucoadhesive Microspheres of Oseltamivir by Ionic Gelation MethodDownload Article

    S.Allimalarkodi, C.Lokesh, V.Ganesan
    • Article Type: Research Article
    • View Abstract
    • Pages (1-12)
    • No of Download = 1350

    Abstract

    close

    The objective of the present study was to prepare and evaluate the mucoadhesive microspheres of oseltamivir. Oseltamivir microspheres were prepared by ionotropic gelation method using polymers such as HPMC (K 100 M), carbopol 940P, sodium CMC, sodium alginate. Totally 10 different formulations of oseltamivir were prepared by using the above polymers. The microspheres were characterised for drug content, entrapment efficiency, swelling index, mucoadhesive property by in vitro wash-off test and in-vitro drug release. The formulation F8 was selected as an ideal formulation based on the in vitro release profile which showed a controlled drug release of 86.11% up to 12 hours in acidic buffer of pH 1.2. Surface morphology (SEM analysis) and drug-polymer interaction studies (FT-IR analysis) were performed only for all the formulations. The microspheres were smooth and elegant in appearance showed no visible cracks as confirmed by SEM and FT-IR studies indicated the lack of drug-polymer interactions in the ideal formulation F8. The in vitro release data of all microsphere formulations were plotted in various kinetic equations to understand the mechanisms and kinetics of drug release. The ideal formulation, F8 followed Higuchi model kinetics.

  2. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Rosuvastatin and Ezetimibe in Tablet Dosage FormDownload Article

    Vadthya Rajashekar, K.Rajeswar Dutt, N.Ramathilagam.
    • Article Type: Research Article
    • View Abstract
    • Pages (13-21)
    • No of Download = 857

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Rosuvastatin and Ezetimibe in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6mm x 100mm, 5m)column at ambient temperature with a mixture of ortho phosphoric acid buffer and Acetonitrile in the ratio 40:60 v/v as mobile phase, at a flow rate of 1.0 mL min-1. UV detection was performed at 237 nm.. The retention times of Rosuvastatin and Ezetimibe were 2.490 and 3.173 min, respectively. The correlation coefficient of Rosuvastatin and Ezetimibe was found to be 0.999. Calibration plots were linear over the concentration ranges 10–50 μg mL-1 for Rosuvastatin and Ezetimibe, respectively. The Limit of detection was 1.626 and 0.918µg mL-1 and the quantification limit was 4.927 µg mL-1 and 2.783µg mL-1 for Rosuvastatin and Ezetimibe, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.59% to 100.70%.The method was validated for accuracy,linearity,sensitivity,precision,robustness,system suitability Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of Rosuvastatin and Ezetimibe in pharmaceutical tablet dosage form.

  3. Oral Dispersible Tablets - A ReviewDownload Article

    P.Venkateshwar Reddy, Swagata Dutta Roy, G.Vasavi, N.Sriram
    • Article Type: Review Article
    • View Abstract
    • Pages (22-29)
    • No of Download = 1635

    Abstract

    close

    The convenience of administration and improved patients compliance are important in the design of oral drug delivery s system which remains the preferred route of drug delivery inspite of various disadvantage.one such problem can be solved in the novel drug delivery system by formulating oral disintegrating tablets which disintegrates rapidly without water within few seconds in the mouth due to action of super disintegrants in the formulation. Oral disintegrating tablets are advantageous for pediatric, geriatric mentally ill, nausea patients who have difficulty in swallowing conventional tablets and capsules. Using various excipients, evaluation tests marketed formulation and drugs used in the research area.

  4. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Etodolac and Paracetamol in Tablet Dosage FormDownload Article

    Tabassum, K.Pranitha, J.Priya, N.Ramathilagam.
    • Article Type: Research Article
    • View Abstract
    • Pages (30-37)
    • No of Download = 856

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Etodolac and Paracetamol in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6mm x 150mm,5m) column at ambient temperature with a mixture of mixed ortho phosphoric acid buffer and Acetonitrile in the ratio 50:50 v/v OPA buffer ; as mobile phase, at a flow rate of 0.9 mL min-1. UV detection was performed at 260 nm. The method was validated for accuracy, precision, linearity and sensitivity. The retention times of Etodolac and Paracetamol were 2.190 and 3.373 min, respectively. Calibration plots were linear over the concentration ranges 4–20 μg mL-1 and 5-25μg mL-1 for Etodolac and Paracetamol, respectively. The Limit of detection was 0.361 and 0.559µg mL-1 and the quantification limit were 1.203 µg mL-1 and 1.864µg mL-1 for Etodolac and Paracetamol, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.23% to 99.93%. Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of Etodolac and Paracetamol in pharmaceutical tablet dosage form.

  5. Validated UV Method Development for the Simultaneous Estimation of Rabeprazole sodium and Cinitapride in TabletsDownload Article

    Shanta Kumari Adiki, Kommineni Lahari, Baishakhi Dey, Abdurraouf M.M.Khalf, Shukri M.O.Al-Sharif, Saousen R. Diaf, Prakash Katakam, Babu Rao Chandu.
    • Article Type: Research Article
    • View Abstract
    • Pages (38-45)
    • No of Download = 1180

    Abstract

    close

    Current research attempts to develop simple, cost effective, and time saving, validated UV spectrophotometric method for the simultaneous estimation of Rabeprazole (RPZ) and Cinitapride (CTP) in tablet formulations by simultaneous equation method. The sampling wavelengths for RPZ and CTP are 284.5 nm and 267 nm respectively. Assay results showed 10.008 mg of RPZ and 2.974 mg of CTP were found in the tablet dosage form. The method was validated as per ICH guidelines. Linearity was obtained in the concentration range of 3-8 μg/mL for RPZ and 2-7 μg/mL for CTP. The %RSD for intraday and interday variations of RPZ was found to be 0.183±0.002 and 0.317±0.001 respectively. An intraday and interday variation of CTP was found to be 0.194±0.002 and 0.298±0.001 respectively. In both cases values were within the acceptance limit of < 2%. The mean percent recovery for RPZ and CTP were found to be 98.57 % and 99.43 % respectively, within the acceptance limit of 98% to 102%. From the high recovery values (> 98%) it can be inferred that the method is free from the interference of excipients used in the formulation. Based on the results obtained the proposed method can be regarded as simple, accurate, precise, reliable and cost effective which can be employed for routine quality control of RPZ and CTP in combined tablet dosage forms.

  6. Method Development and Validation of RP-HPLC Method for Glimepiride, Pioglitazone Hcl and Metformin Hcl in TabletsDownload Article

    N.Ramathilagam, P.Solairaj.
    • Article Type: Research Article
    • View Abstract
    • Pages (46-54)
    • No of Download = 1674

    Abstract

    close

    A simple, accurate, precise and linear Isocratic RP-HPLC has been developed and subsequently simultaneous for the determination of glimepiride, pioglitazone Hcl and metformin Hcl in pharmaceutical dosage form. Kromosil C18 (150mm X4.6 mm) 5µ with flow rate of 1ml/min by using JASCO PU-1580 and UV/VIS JASCO UV-1570 at 217nm. The separation was carried out using a mobile phase consisting of the mixture of methanol and 25mM phosphate buffer (pH-2.0) in the ratio of 50 :50. The retention time for glimepiride, metformin Hcl and pioglitazone Hcl were found to be 2.24, 3.76 and 10.20 min. the correlation coefficient was found to be 0.999 for GLI, 0.9993 for PIO and 0.9997 for MET. The mean percentage recovery was found to be 98.58 (GLI), 98.30 (PIO) and 98.87 (MET). The percentage the accuracy of the drugs were found to be near to 100% representing the accuracy of the method. The proposed method was also validated and applied for the analysis of the drugs on tablet formulations.

  7. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Aceclofenac and Thiocolchicoside in Tablet Dosage FormDownload Article

    S.Venkata Subba Reddy, K.Rajeswar Dutt
    • Article Type: Research Article
    • View Abstract
    • Pages (55-66)
    • No of Download = 1625

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Aceclofenac and Thiocolchicoside in tablet dosage form. Chromatographic analysis was performed on a Symmetry Nucleosil C8 (150X4.6 mm,5µm) column ambient temperature with a mixture of mixed phosphate buffer and Acetonitrile in the ratio 40:60(buffer preparation: Prepare 0.01M Disodium hydrogen orthophospate as mobile phase, at a flow rate of 0.80 mL min-1. UV detection was performed at 261 nm. The method was validated for accuracy, precision, specificity, linearity and sensitivity. The retention times of Aceclofenac and Thiocolchicoside were 2.167 and 4.866 min, respectively. The Limit of detection was 0.33 and 3.9 µg mL-1 and the quantification limit was 1.002 µg mL-1 and 11.9 µg mL-1 for Aceclofenac and Thiocolchicoside respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.94% to 99.81%.

  8. A New Validated Stability-Indicating RP-HPLC Method for the Estimation of Pitavastatin in Tablet Dosage FormsDownload Article

    K.Sujatha, J.V.L.N.Seshagiri Rao.
    • Article Type: Research Article
    • View Abstract
    • Pages (67-74)
    • No of Download = 682

    Abstract

    close

    An accurate and stability - indicating high performance liquid chromatographic method was developed for quantification of pitavastatin in its tablet dosage forms. Ideal separation of the drug was achieved on an Agilent Eclipse XDB C18 column (150 x 4.6 mm; 5m) by eluting with a mobile phase consisting of a mixture of phosphate buffer (pH 3.4) and acetonitrile (65:35 v/v) at a flow rate of 0.9 mL/min. The drug in the eluates was monitored at 244 nm. Under optimized conditions, the retention time obtained for the drug was 3.905 min. The calibration plot was linear in the concentration range of 25 - 150 µg/mL of the drug. The validation of the method was done by following the ICH guidelines. The proposed method could be applied for determination of pitavastatin in its tablet dosage forms without any interference from normal excipients. The method thus, is suitable for routine quality control analysis of pitavastatin.

  9. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Metoprolol and Telmisartan in Tablet Dosage FormDownload Article

    K.Pranitha, Tabassum, J.Priya, N.Ramathilagam.
    • Article Type: Research Article
    • View Abstract
    • Pages (75-82)
    • No of Download = 878

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Metoprolol and Telmisartan in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6 mm x 100 mm, 5 m) column at ambient temperature with a mixture of ortho phosphoric acid buffer and Acetonitrile, Methanol in the ratio 45:10:45 v/v as mobile phase, at a flow rate of 0.7 mL min-1. UV detection was performed at 226 nm. The retention times of Metoprolol and Telmisartan were 2.473 and 3.407 min, respectively. The correlation coefficient of Metoprolol and Telmisartan was found to be 0.999. Calibration plots were linear over the concentration ranges 12.5–62.5 μg mL-1 and 10-50 μg mL-1 for Metoprolol and Telmisartan, respectively. The Limit of detection was 0.667 and 0.846µg mL-1 and the quantification limit was 2.021 µg mL-1 and 2.565 µg mL-1 for Metoprolol and Telmisartan, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.93% to 101.09%.The method was validated for accuracy, linearity, sensitivity, precision, robustness, system suitability Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of Metoprolol and Telmisartan in pharmaceutical tablet dosage form.

  10. RP-HPLC Method Development and Validation for the Simultaneous estimation of Simvastatin and Ezetimibe in Tablet Dosage FormDownload Article

    Mohammed. Ibrahim, K.Rajeswar dutt, Vadthya Rajashekar.
    • Article Type: Research Article
    • View Abstract
    • Pages (83-91)
    • No of Download = 500

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Simvastatin and Ezetimibe in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry C8 (4.6mm x 150mm, 5m) column at ambient temperature with a mixture of ortho phosphoric acid buffer and Acetonitrile in the ratio 40:60 v/v (Ortho phosphoric acid buffer preparation; Take 1000ml of HPLC grade water and 1 ml orthophosphoric acid and pH adjusted) as mobile phase, at a flow rate of 1.0 mL min-1. UV detection was performed at 221 nm. The method was validated for accuracy, precision, specificity, linearity and sensitivity. The retention times of Simuvastatin and Ezetimibe were 2.190 and 3.515 min, respectively. Calibration plots were linear over the concentration ranges 10–50 μg mL-1 and 10-50 μg mL-1 for Simvastatin and Ezetimibe, respectively. The Limit of detection was 2.0 and 6.31µg mL-1 and the quantification limit were 6.31 µg mL-1 and 2.99 µg mL-1 for Simvastatin and Ezetimibe, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.98% to 101.21%..

  11. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Paracetamol and Tramadol Hydrochloride in Tablet Dosage FormDownload Article

    K.Shivaramakrishna, K.Rajeswar dutt, Vadthya Rajashekar
    • Article Type: Research Article
    • View Abstract
    • Pages (92-101)
    • No of Download = 813

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Paracetamol and Tramadol hydrochloride in tablet dosage form. Chromatographic analysis was performed on a Symmetry Thermo C18(150X4.6 mm,5µm) column ambient temperature with a mixture of mixed phosphate buffer and Acetonitrile in the ratio 60:40 (mixed phosphate buffer preparation; 0.01 M Potassium dihydrogen orthophosphate, pH 3.5 adjust with triethylamine) as mobile phase, at a flow rate of 0.80 mL min-1. UV detection was performed at 268 nm. The method was validated for accuracy, precision, specificity, linearity and sensitivity. The retention times of Paracetamol and Tramadol hydrochloride were 2.250 and 3.378 min, respectively. Calibration plots were linear over the concentration ranges 62.500-375.000 μg mL-1 and 6.25-37.50 μg mL-1 for Paracetamol and Tramadol hydrochloride respectively. The Limit of detection was 1.8704 and 1.254 µg mL-1 and the quantification limit was 5.6679 µg mL-1 and 3.8008 µg mL-1 for Paracetamol and Tramadol hydrochloride respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.56% to 100.55%.

  12. Process Analytical TechnologyDownload Article

    Satish kumar kengam
    • Article Type: Review Article
    • View Abstract
    • Pages (102-108)
    • No of Download = 1380

    Abstract

    close

    The increasing demand for the better healthcare products has changed the pharmaceutical industry. In the recent years, the technologies producing the quality products have paved the way for good quality products. The introduction of new tools and technologies has provided an opportunity for the pharmaceutical producers to improve the quality standards of the product. PAT is one of the technologies in pharmaceutical production where they test out the quality of the raw materials, characterize them both physically and chemically, through at-line, in-line or on-line. PAT saves time and money required for testing and analyzing the products. The PAT paves the way for making good quality products thus satisfies the customers needs and build a good brand image for the organization. The two effective tools NIR and RAMAN spectroscopy are used in testing the quality of the products. This research essay delves the essentials of PAT and the usefulness of process analyzers in process monitoring. The effectiveness of the PAT tools and the advantages of NIR over RAMAN spectroscopy are clearly discussed.

  13. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Atenolol and Indapamide in Pharmaceutical Tablet Dosage FormDownload Article

    K.Madhavi, K.Deepti, B.Harika
    • Article Type: Research Article
    • View Abstract
    • Pages (109-117)
    • No of Download = 702

    Abstract

    close

    A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of ATENOLOL and INDAPAMIDE in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6mm x 100mm, 5m) column at ambient temperature with a mixture of Potassium di hydrogen phosphate buffer and Acetonitrile in the ratio 40:60 v/v as mobile phase, at a flow rate of 0.7 mL min-1. UV detection was performed at 240 nm. The retention times of Atenolol and Indapamide were 2.1 and 3.6 min, respectively. The correlation coefficient of Atenolol and Indapamide was found to be 0.999. Calibration plots were linear over the concentration ranges 20–100 μg mL- 1 and 1-5 μg mL- for Atenolol and Indapamide respectively. The Limit of detection was 0.223 and 0.286µg mL-1 and the quantification limit were 0.677 µg mL-1 and 0.86µg mL-1 for Atenolol and Indapamide, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 100.74% to 99.93%.The method was validated for accuracy, linearity, sensitivity, precision, robustness, system suitability Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of Atenolol and Indapamide in pharmaceutical tablet dosage form.

  14. Consumer’s Perception Regarding Pharmaceutical Product Packaging: A Survey of PakistanDownload Article

    Arif Sabah, Atta Abbas, Sidra Tanwir, Farrukh Rafiq Ahmed, Adeel Arsalan, Aysha Arif, Syed Imran Ali, Shazia Adnan, Sarah Haroon and Syed Ata Rizvi.
    • Article Type: Research Article
    • View Abstract
    • Pages (118-125)
    • No of Download = 1142

    Abstract

    close

    The packaging of the pharmaceutical product plays an important role to maintain the product stability and an increase in sales which ultimately increase in profit margin of any organization. The packaging continuously provides benefits and creates awareness for the brand preferences. Any country’s export would not be the international standard until and unless the product with its packaging does not meet the international standard of packaging for its design and product safety and stability. In any market, the product may be of tremendous quality but it will never attain the consumer’s acceptance unless the packaging of the product is of excellent quality to maintain the product quality for distribution and manufacturer’s integrity. The foreign products are packed in good packaging materials with much colorful presentation and artistic value which are missing in the packaging of Pakistani pharmaceutical products. It is an fact that most of the Pakistani made products have not succeeded to gain the local market share due to the substandard packaging quality. For international highly sophisticated market, the Pakistani products and their package quality will have to compete at their level to fulfill their market share. The common reasons given for the low patronage of Pakistan’s pharmaceutical product is that, they are less attractive and substandard and local consumers have a general perception that the packaging of product manufactured in Pakistan does very little to promote the products. There is a strong relationship between the customers and packaging to easily identify Pakistan’s pharmaceutical products is the first step to capture the local market and increase the competiveness of Pakistani products in the international market.

  15. Method Development and Validation for Simultaneous estimation of Metformin Hcl and Sitagliptin by RP-HPLC in Tablet Dosage FormDownload Article

    Sudheer Kumar Sapavat, V.Mohan goud, J.V.C Sharma.
    • Article Type: Research Article
    • View Abstract
    • Pages (126-134)
    • No of Download = 577

    Abstract

    close

    A simple, accurate, precise and rapid reversed-phase high performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous estimation of Metformin Hydrochloride and Sitagliptin Phosphate in pure and tablet formulation. The proposed method is based on the separation of the two drugs in reversed-phase mode using zodiac C18 (250×4.6 mm, 5 μm particle size). The optimum mobile phase consisted of phosphate buffer : acetonitrile in the ratio of 55:45 v/v (Phosphate buffer pH 5.8 was adjusted with sodium hydroxide) was selected as a mobile phase, flow rate of 1.0 ml/min and UV detection was set at 244 nm. The retention times were 2.1 and 4.90 min for Metformin Hydrochloride and Sitagliptin Phosphate respectively. The method was validated according to ICH guidelines. It was found to be accurate and reproducible. Linearity was obtained in the concentration range of 75-175 μg/ml for Metformin Hydrochloride and 7.5-17.5 μg/ml Sitagliptin Phosphate. Mean percent recovery of samples at each level for both drugs were found in the range of 99.70% for Metformin Hydrochloride and 99.40s % for Sitagliptin Phosphate. The proposed method can be successfully applied in the quality control of bulk and pharmaceutical dosage forms.

  16. Formulation and Evaluation of Microspheres of CefdinirDownload Article

    S.Janet Beula, A.Swathi, Sukantha satapathy, Rajaram Das, N.Sruthi, D.Swetha.
    • Article Type: Research Article
    • View Abstract
    • Pages (135-139)
    • No of Download = 611

    Abstract

    close

    Microspheres are sub-micron size polymeric drug carrier systems in which the therapeutic agents are loaded in micrometer. These particles consist of core material, which is the drug, and a coating material. Microspheres are considered as a very promising controlled and targeted drug delivery system. The formulation and clinical application of microspheres is based on the physicochemical, pharmacokinetic and pharmacological properties of drugs. Cefdinir is a beta-lactam antibiotic and is mainly bactericidal. Cefdinir inhibits the third and final stage of bacterial cell wall synthesis by preferentially binding to specific penicillin binding proteins, those are located inside the bacterial cell wall. Cefdinir is a third generation anti-biotic used for the treatment of community -acquired pneumonia, acute bacterial otitis media and uncompleted skin and skin structure infections in adult and pediatric patient. Incorporation of Cefdinir in polymeric microspheres can successfully increase the biological half-life and reduce the therapeutic dose of their drug, thereby minimizing the adverse drug reaction. Cefdinir microspheres were formulated by emulsion solvent evaporation method using ethyl cellulose polymer. All the above studies reveal that the microsphere can serve as an ideal drug delivery system for Cefdinir. Further studies can be done on the stability of cefdinir-loaded microspheres and the improvement in therapeutic efficacy due to the targeting effect on to the specific receptor sites.

  17. Synthesis and screening of new 2-(2-oxoindoline-3-ylidene)-n- Phenyl hydrazine carbothioamidesDownload Article

    R Asha, B Babu Rao, B Rama Rao
    • Article Type: Research Article
    • View Abstract
    • Pages (140-146)
    • No of Download = 663

    Abstract

    close

    The purpose of this work to prepare or to synthesize the little compound, 2-(2-oxoindoline-3-ylidene)-N-phenyl hydrazine carbothioamide derivatives by adopting appropriate synthetic routes. Then Purification and characterization of all the new compounds including those of intermediates by recrystallization from appropriate solvents or by chromatographic techniques. And Characterization of the newly synthesized compounds by physical and spectral methods (IR, 1HNMR & MASS). Finally evaluation of the new compounds for antimicrobial activities by standard methods available in the literature. It is evident from literature that the presence of the indole nucleus found to have various pharmacological activities like antimicrobial, anti-convulsant, MAO inhibitory, anticancer and psychotropic activities. The very fact that one of indole derivatives (isatins) is potential synthon, for building synthetically a variety of chemical systems known for their broader and pharmacological applications. In view of pharmacological significance of indole derivatives is planned to synthesize some new indole derivatives containing thiosemicarbazides.

  18. Analytical Method Development and Validation for Simultaneous Estimation of Lercandipine and Atenolol Tablet Dosage Form by RP-HPLCDownload Article

    Mahaboob Subhani Syed, Ch.Bala Sekhar Reddy, M.Sarbudeen.
    • Article Type: Research Article
    • View Abstract
    • Pages (147-156)
    • No of Download = 696

    Abstract

    close

    A rapid high performance liquid chromatographic method has been development and validation for the estimation of Lercanidipine and Atenolol stimultaneously in combined dosage form. A kromosil C-8 column having dimensions of 4.6µmx250mm and particle size of 5µm in isocratic mode , with mobile phase containing a mixture of Di- Potassium Hydrogen Phosphate and Acetonitrile in ratio of (70:30v/v) (pH adjusted to pH 6.5±0.5 using phosphoric acid was used . Detection was done at 215 nm using PDA detector. The mobile phase was pumped at a flow rate of 1.0µl/min and injection volume is10µl. The selected chromatographic conditions were founds to effectively separate Lercandipine (Rt 3.8min)(2) and Atenolol (Rt: 6.1 min) having resolution of 7.8. The method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantitation.

  19. A label-free electrochemical protein sensor of perchloric acid doped polyanilineDownload Article

    Manju Singh, Sandeep Yadav.
    • Article Type: Research Article
    • View Abstract
    • Pages (157-168)
    • No of Download = 761

    Abstract

    close

    In this study, we developed a potentiometric based protein sensor utilizing the interactions between charged functional moieties of a target protein-protein interaction and the complexation between PANI and dopant molecules. The sensor response depended on the isoelectric point of the protein. However, this dependency may be exploited to enhance specificity of protein sensors at specific pH dependence. The conducting polymer was found to respond by changing potential in the presence of biomolecules, demonstrating a direct chemical to electronic transduction method. The influence of polymer surface and morphology of finished films was also studied. This study demonstrated a conducting polymer able to respond to proteins at physiological conditions, in other words a step towards the integration of these materials into implantable sensing system. Additionally this sensor also had better ability to recognize the specific protein-protein interaction.

  20. Design and evaluation of Bilayered tablets of SimvastatinDownload Article

    Anna balaji, Sreekanth Goud.P.
    • Article Type: Research Article
    • View Abstract
    • Pages (169-177)
    • No of Download = 1459

    Abstract

    close

    The Objective of this study was to formulate bilayer tablets comprising of Simvastatin in both Extended Release layer and immediate release layer and to carry out in vitro dissolution studies for the formulated tablets as per official specifications. Bilayer tablets comprised two layers, i.e. immediate release and extended release layer. Simvastatin is an orally active anti hypertensive agent. For immediate release drug and polymer ratio (Simvastatin: croscarmellose sodium in the formulations I1 to I3 was prepared in the ratio (1:1, 1:2, 1:3). Whereas for extended release, drug and polymer ratio (Simvastatin: HPMC K4M) were formulated as 1:0.5, 1:1, 1:1.5, 1:2 and 1:2.5 in Formulations C1 to C5. The immediate release layer comprised sodium starch glycolate and croscarmellose sodium as super disintegrant and sustained release layer comprised ethyl cellulose and HPMC K4M as release retardant polymers. Direct compression method was used for formulation of bilayer tablets. Accelerated stability studies were carried out in accordance with ICH guidelines. Ethyl cellulose and HPMC K4M retarded the release of Simvastatin from the controlled release layer for 12 hrs. After stability tests, degradation of both drugs were found but the drugs, contents were found to be within the range. Drug release mechanism release exponent (n) were determined for all formulations (0.689-0.789). The immediate release layer of Simvastatin was found to follow a first order release model and the extended release layer of Simvastatin was found to follow zero order release model.

  21. Development and Validation of RP-HPLC method for the simultaneous estimation of Miglitol and Metformin Hcl in Pharmaceutical formulationsDownload Article

    Srinivas Ampati, Sandeep Dyavarashetti, Kiran Gangarapu.
    • Article Type: Research Article
    • View Abstract
    • Pages (178-184)
    • No of Download = 1540

    Abstract

    close

    A simple, accurate, precise and rapid reversed-phase high performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous estimation of Miglitol (MG) and Metformin Hydrochloride (MT) and in pure and tablet formulation. The proposed method is based on the separation of the two drugs in reversed-phase mode using zorabaxC18 column (250×4.6 mm I.D., 5 μm particle size). The optimum mobile phase consists of Phosphate buffer of (pH 4.0): Methanol in the ratio of 80:20 v/v was selected as a mobile phase, flow rate is 1.0 ml/min and the αmax of UV detection was set at 251 nm. The retention times were observed at 3.045 and 4.460 min for Miglitol and Metformin Hydrochloride respectively. The method was validated according to ICH guidelines. The method could be accurate and reproducible. Linearity was obtained in the concentration range of 50-150% for Miglitol and 50-150% Metformin Hydrochloride. Mean percent recovery of samples at each level for both drugs were found in the range of 100%. The proposed method could be successfully applied in the quality control of bulk and pharmaceutical dosage forms.

Sign in

Registered and Approved by National Science Library (NSL),National Institute of Science Communication And Information Resources(NISCAIR),
Council of Scientific and Industrial Research,New Delhi, India"