IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

Search form

Articles

  1. Validated stability indicating RP-HPLC method for the simultaneous determination of ofloxacin, ornidazole, clobetasol propionate, terbinafine hydrochloride, methyl paraben, propyl paraben in bulk and pharmaceutical dosage formDownload Article

    Bommadevara. Priyanka, Sk. Abdul Rahaman
    • Article Type: Research Article
    • View Abstract
    • Pages (301-318)
    • No of Download = 2103

    Abstract

    close

    A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Ofloxacin (OFL) ,Ornidazole (ORD), Terbinafine hydrochloride (TFH) , clobetasol propionate(CLP) , Methyl paraben(MP) ,propyl paraben(PP) in bulk and pharmaceutical dosage forms. The method was carried out on a Zodiac C18 (250mm x 4.6mm, 5µm) column with a mobile phase consisting of Ortho phosphoric acid buffer, PH 2.5 and Acetonitrile in the ratio (82:18v/v) and flow rate of 1ml/min .The detection was carried out at 255nm. The retention time for estimation of ofloxacin (0.712min), ornidazole(1.933min), Terbinafine hydrochloride (7.302min), clobetasol propionate (9.224min), Methyl paraben (4.074min), propyl paraben(7.926min) .The Linearity of proposed method was investigated in the range of 1-960 µg/ml with r2 value for ofloxacin (0.999), ornidazole(0.999), Terbinafine hydrochloride (0.999), clobetasol propionate (0.998), Methyl paraben (0.998), propyl paraben(0.997) .The amount of drug estimated by the proposed method was found to be in good agreement with label claim. The developed method was validated for precision, accuracy, sensitivity, robustness and ruggedness. Hence it can be applied for routine analysis of titled drug in bulk and pharmaceutical formulations.

  2. Method development and validation for the simultaneous estimation of sitagliptin and metformin in tablet dosage form by RP-HPLCDownload Article

    B. Thangabalan, *N. Mamatha, S. Manohar Babu.
    • Article Type: Research Article
    • View Abstract
    • Pages (319-325)
    • No of Download = 704

    Abstract

    close

    A RP-HPLC method was developed and validated for the simultaneous estimation of Metformin Hydrochloride (MET) and Sitagliptin (STG) in pure and pharmaceutical dosage form. Chromatography was carried on Phenomex (kromosil-250 mm × 4.6 mm, 5 μm) column with mobile phase comprising of phosphate buffer and acetonitrile in the ratio 75:25 v/v. The flow rate was adjusted to 1.0 ml/min with UV detection at 260 nm. The retention times of MET and STG were found to be 1.43 min, 2.3 min respectively. The different analytical parameters such as accuracy, linearity, precision, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined according to the ICH-Q2B guidelines. The detector response was linear in the range of 25-250 μg/ml, 2.5-25 μg/ml for MET, STG respectively. The proposed RP-HPLC method is sensitive, precise and accurate so it was successfully applied for the reliable quantification of drugs in the commercial dosage form.

  3. Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage formDownload Article

    Nirupama.D, P.Venkateswara Rao, B.Thangabalan, S.Manohar Babu.
    • Article Type: Research Article
    • View Abstract
    • Pages (326-333)
    • No of Download = 974

    Abstract

    close

    A simple, precise, accurate and rapid reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Gliclazide(GLZ) and Metformin(MET) in pure and tablet dosage form. The method was carried on Phenomex (kromosil-250 mm × 4.6 mm, 5 μm) column with mobile phase comprising of phosphate buffer and methanol in the ratio 60:40 v/v. Flow rate was adjusted to 1.0ml/min and effluents were monitored at 230 nm. The retention time obtained for Gliclazide and Metformin was 5.0 and 3.2 mint respectively. The calibration curves were linear in the concentration range of 10-100μg/ml for Gliclazide and Metformin 62.5-625μg/ml for. The developed method was validated in accordance to ICH guidelines.

  4. Analytical method development and validation for simultaneous estimation of naproxen and pantoprazole in capsule dosage form by RP-HPLCDownload Article

    Mahaboob subhani.syed
    • Article Type: Research Article
    • View Abstract
    • Pages (334-347)
    • No of Download = 986

    Abstract

    close

    A simple, rapid, precise, accurate RP-HPLC method has been developed and validated for the simultaneous estimation of Naproxen and Pantoprazole in combined dosage forms. Chromatographic separation was achieved with mobile phase consisting of Methanol: Phosphate Buffer PH 5.4in the ratio of 70:30 v/v with Hypercil C18 (250 × 4.6 mm × 5 μm),column at a flow rate of 1 mL/minand detection wavelength was 259 nm. The retention times of Naproxen and Pantoprazole was found to be 3.33 minand 1.90 minrespectively. The method was validated in terms of Linearity, Range, Accuracy, Precision, Specificity, LOD, LOQ, Robustness and system suitability according to ICH guidelines. Commercial Capsule formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis for determination of Naproxen and Pantoprazole in Capsule dosage form.

  5. Formulation of mebeverine hydrochloride MR pellets in capsules and comparative characterization against colofac MR capsulesDownload Article

    S.Petchimuthu, N. Narayanan, Subashini Uthirapathy
    • Article Type: Research Article
    • View Abstract
    • Pages (348-362)
    • No of Download = 4232

    Abstract

    close

    The aim and objective of the present study is to develop Mebeverine Hydrochloride MR pellets in the form of capsule. Pellets with coating for Modified Release have a lower risk of dose dumping than coated tablets. Modified Release capsules of Mebeverine HCl were formulated by using the solid drug layering (SDL) process by dusting the Drug Excipients mixture on inert MCC pellets by using Povidone K-30 + IPA solution as binder. The drug layered pellets were coated by using the Eudragit S-100, PEG 6000 and Talc dispersed in purified water in order to modify the drug release. The coated pellets are filled in capsules and these capsules were evaluated for assay, weight variation, content uniformity, lock length, moisture content and in-vitro dissolution tests and all within the specification limit. There is no physicochemical interaction between drug and excipient which was proven by compatibility study results which was carried out for 4 weeks at Accelerated stability (AS) condition. The optimized batch F7 is kept under AS conditions (40°C/75%RH) and the product is monitored and analyzed for assay, moisture content, content uniformity, in-vitro dissolution study and all the parameters were well within the specification limit. The release rate was compared with the Reference product “Colofac MR” (Abbot Pharmaceuticals Ltd) and F1 & F2 values were within the limit. And it is pharmaceutically equivalent to that of the reference

  6. Method development and validation for the simultaneous estimation of saxagliptin and metformin in tablet dosage form by RP-HPLC methodDownload Article

    B. Thangabalan, P.Srisowmya, S. Manohar Babu
    • Article Type: Research Article
    • View Abstract
    • Pages (363-369)
    • No of Download = 880

    Abstract

    close

    A RP-HPLC method was developed and validated for the simultaneous estimation of MetforminHydrochloride (MET) and Saxaagliptin (SGL) in pure and pharmaceutical dosage form. Chromatography was carried on PhenominexC18(250 mm × 4.6 mm, 5 μm) column with mobile phase comprising of phosphate buffer and acetonitrile in the ratio( 60:40) v/v. The flow rate was adjusted to 0.7 ml/min with UV detection at 242 nm. The retention times of MET and SGL were found to be 1.7 min, 2.9 min respectively. The different analytical parameters such as accuracy, linearity, precision, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined according to the ICH-Q2B guidelines. The detector response was linear in the range of 100-600μg/ml, 1-6 μg/ml for MET, SGL respectively. The proposed RP-HPLCmethod is sensitive, precise and accurate so it was successfully applied for the reliable quantification of drugs in the commercial dosage form.

  7. Formulation and evaluation of matrix type rosuvastatin sustained release tabletsDownload Article

    Sreelatha.P, S.Manohar Babu,Y.Raja Jaya Rao
    • Article Type: Research Article
    • View Abstract
    • Pages (370-383)
    • No of Download = 1550

    Abstract

    close

    Sustained release tablets of Rosuvastatin were developed to prolong release of time leading to an increase in drug bioavailability.Tablets are prepared by direct compression technique using polymers HPMC K15M,HPMC K50M and ethylcellulose.Tablets were evaluated for their physical characteristics viz.,hardness, thickness, friability and weight variation, drug content and floating properties.Gas generating agent plays an important role in floating lagtime and drug release.The best formulation subjected for kinetic treatment.i.e., zero order, first order, pepas, Higuchi,and Hixsoncro well. The RValues are 0.9366, 0.9364, 0.9680, 0.9974 and 0.9283 respectively. The optimized batches were found to best able at 400C/75% RH for a period of two months.

  8. Total phenolic, flavonoids and tannin content of various extracts from Pyrus communis fruitDownload Article

    Velmurugan C and Anurag bhargava
    • Article Type: Research Article
    • View Abstract
    • Pages (384-390)
    • No of Download = 1004

    Abstract

    close

    The pyrus communis commonly known as Pear fruit having numerous pharmacological properties. Natural bioactive compounds like phenols, tannin and flavonoids are the important secondary metabolites in plant possess wide range biological action and this will supported with scientific studies on these metabolite from pear fruit. To maximize these agents in the extract different solvents viz. chloroform, ethyl acetate, ethanol and aqueous are used for the extraction procedure. Current study was aimed to determine the levels of total phenolic, flavonoids and tannin contents. Observations suggested that ethyl acetate and ethanol extracts has significantly high (P<0.001) concentration of flavonoids, phenolic and tannin contents as compared to aqueous and chloroform extracts. Therefore, ethyl acetate and ethanol extracts of pyrus communis has greater potential to produce more beneficial effects in biological system as compared to aqueous and chloroform extracts.

  9. LC method development and validation of aspirin and clopidogrel in pure API’S and its pharmaceutical dosage formsDownload Article

    P.Praveen Kumar, I.Srikanth, K.Anusha,D.Srinivasa Rao.
    • Article Type: Research Article
    • View Abstract
    • Pages (391-396)
    • No of Download = 764

    Abstract

    close

    A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of Aspirin and Clopidogrel in pure sample and its pharmaceutical dosage form. The mobile phase consists of Phosphate buffer (0.02 M Potassium dihydrogen phosphates, pH-3 adjusted with ortho phosphoric acid): acetonitrile in the ratio of 65:35 v/v delivered at a flow rate of 1.0 ml / min and wavelength of detection at 229 nm. The retention times of Aspirin and Clopidogrel were 2.673 and 3.627min respectively. The developed method was validated according to ICH guidelines. The result indicates that the method was found to be simple, rapid, and accurate and can be adopted in routine analysis of Aspirin and Clopidogrel in Pure sample and its Pharmaceutical dosage forms.

  10. RP-HPLC method development and validation of doxycycline in bulk and tablet formulationDownload Article

    Jeyabaskaran.M, Rambabu.C, Sree Janardhanan V, Rajinikanth.V, Pranitha.T and Dhanalakshmi.B
    • Article Type: Research Article
    • View Abstract
    • Pages (397-404)
    • No of Download = 1969

    Abstract

    close

    A Simple, accurate and rapid RP-HPLC method has been developed for the estimation of doxycycline (DOXY) in bulk and pharmaceutical dosage forms using an Altima C 18, 150 x 4.6 mm i.d, 5 µm particle size in isocratic mode; with mobile phase comprising of buffer (0.1% of potassium dihydrogen phosphate) and acetonitrile in the ratio 60:40 (v/v). The flow rate was 1 ml/min and detection was carried out by photodiode array detector at 268 nm. The retention time for DOXY was found to be 2-897 min. The proposed method has permitted the quantification of DOXY over linearity in the range of 25 – 150 µg/ml and its percentage recovery was found to be 99.134 – 101.997 %. The % RSD of intraday and inter day precision were found 0.7999% and 1.3%.

  11. Stability indicating method development and validation for the simultaneous estimation of rabeprazole sodium and ketorolac tromethamine in bulk and synthetic mixture by RP-HPLCDownload Article

    Krishanu Pal, Uday Kiran Kumar Nyatha*, R. Ramesh Kumar Reddy, D. Soumya, S. Y. Manjunath
    • Article Type: Research Article
    • View Abstract
    • Pages (405-413)
    • No of Download = 775

    Abstract

    close

    A new, simple, fast and economical stability indicating reverse phase high performance liquid chromatographic method for the simultaneous determination of Rabeprazole sodium (RPZ) and Ketorolac tromethamine (KTR) in bulk and synthetic dosage form. Acetonitrile and potassium dihydrogen phosphate buffer was used as a solvent to extract drugs from the synthetic mixture. Subsequently samples were evaluated directly by simultaneous estimation method by using Inertsil C18 (125×4.6 mm, 5µm) column by isocratic elution by using acetonitrile and potassium dihydrogen phosphate buffer (50:50) as a mobile phase. The simultaneous determination of RPZ and KTR was carried out with a flow rate of 1 mL/min and UV detection at 292 nm wavelength. The method was validated according to ICH guidelines with respect to linearity, precision, accuracy, robustness, ruggedness, specificity and limit of detection and limit of quantification. Further the stability of RPZ and KTR were accessed using various stressed conditions like acidic, alkaline, oxidative, thermal and photolytic degradations in bulk drugs (APIs) and formulation. Degradation products produced as a result of stress studies did not interfere with the detection of RPZ and KTR, so the assay can thus be stability-indicating and can be used for the routine analysis.

  12. Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet dosage form by UV-spectrophotometry and RP-HPLCDownload Article

    R.Srinivasan, K.Lurdhu Mary, G.Lakshmana, D.Rajesh Kumar, B.Rajini
    • Article Type: Research Article
    • View Abstract
    • Pages (414-421)
    • No of Download = 774

    Abstract

    close

    A simple, accurate, rapid, precise and novel UV-Spectrophotometry and Reverse phase High Pressure liquid chromatographic method (RP-HPLC) has been developed and validated for simultaneous determination of Tenofovir Disoproxil Fumerate(TDF) And Emtricitabine(EMT) in combined tablet dosage form. At wavelength 260 nm both drugs have considerable absorbance. The UV-Spectrophotometry method was found to be linear between the range of 5-25 μg/ml for TDF and 7-35 μg/ml for EMT.The selected and optimized mobile phase was Acetonitrile : Phosphate pH 3.5 buffers in the ratio of 60:40 was fixed due to good symmetrical peak. and conditions were flow rate (1.0 ml/minute), wavelength (270 nm), Run time was 5 min. The retention time were found to be 2.85 min and 3.55 min for Tenofovir Disoproxil Fumerate And Emtricitabine respectively. Linearity and range was found to be 3-15 µg/ml for Tenofovir Disoproxil Fumerate and 2-10 µg/ml for Emtricitabine. The proposed chromatographic conditions were found appropriate for the quantitative determination of the drugs.The method was validated for accuracy, precision, specificity, linearity, robustness, sensitivity, LOD and LOQ. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible.

  13. Spectrophotometric methods for quantitative estimation of sertraline hydrochloride from tablet formulationDownload Article

    Ravindra N
    • Article Type: Research Article
    • View Abstract
    • Pages (422-425)
    • No of Download = 553

    Abstract

    close

    Two simple and sensitive visible spectrophotometric methods have been developed for the quantitative estimation of Sertraline Hydrochloride from its tablet formulation. The developed methods are based on formation of chloroform extractable colored complex of drug with alizarin red and tropaeolin ooo. The chloroform extracted complex of drug with alizarin red showed absorbance maxima at 425.0 nm and linearity was observed in the concentration range of 20-90 µg/ml (method-I), with tropaeolin ooo showed absorbance maxima at 485.0 nm and linearity was observed in the concentration range of 3-24 µg/ml (method-II). Results of analysis for both the developed methods were validated statistically and by recovery studies.

  14. Simultaneous estimation of meclizine and nicotinic acid by using RP-HPLCDownload Article

    Dr.R.Srinivasan, K.Lurdhu Mary, D.Rajesh Kumar, P.Aswini
    • Article Type: Research Article
    • View Abstract
    • Pages (426-433)
    • No of Download = 697

    Abstract

    close

    A simple, accurate, rapid, precise and novel Reverse phase High Pressure liquid chromatographic method (RP-HPLC) has been developed and validated for simultaneous determination of Meclizine & Nicotinic acid in pharmaceutical dosage form.  max of Meclizine was 220 nm and Nicotinic acid was 273 nm. The selected and optimized mobile phase was acetonitrile: potassium dihydrogen phosphate buffer (0.02M, pH 3.0) (55:45v/v) and conditions were flow rate (1.0 ml/minute), wavelength (234 nm), Run time was 20 min. The retention time were found to be 3.01 min and 6.07 min for Meclizine & nicotinic acid respectively. Linearity and range was found to be 0-140 µg/ml for Nicotinic acid and 0-150 µg/ml for Meclizine. The proposed chromatographic conditions were found appropriate for the quantitative determination of the drugs. The method was validated for accuracy, precision, specificity, linearity, robustness, sensitivity, LOD and LOQ. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible.

  15. Estimation of olopatadine hydrochloride by RP–HPLC and U.V spectrophotometry method in pure and pharmaceutical formulationDownload Article

    Bhanu Prakash Nayak.M, B.Thangabalan
    • Article Type: Research Article
    • View Abstract
    • Pages (434-444)
    • No of Download = 678

    Abstract

    close

    A simple and sensitive Reversed Phase High Performance Liquid Chromatographic method and UV Spectrophotometry has been developed and validated for the of the Olopatadine Hcl in pure and pharmaceutical dosage form. In RP-HPLC Method the separation was carried out using mobile phase consisting of Methanol and Acetonitrile in the ratio of 60:40 (v/v). The column used was inertsil ODS 3V C18, (250 mm x 4.6 mm i.d., 5μm) with flow rate 1.2ml/min using UV detection at 254 nm. The method was linear over a concentration range of 10 – 250μg/. The retention time of 2.857min. Results of analysis were validated statistically and by recovery studies. The mean recovery was 98.2 to101.5. The limit of detection (LOD) and the limit of quantification (LOQ) were found to be 0.024 and 0.075μg/ml. The %RSD for the method precision was found to be less than 2%. To develop simple and economical UV spectrophotometric method for the estimation of Olopatadine in pharmaceutical dosage form available in the market for conjunctivitis.The method was validated as per the ICH guidelines and the results were statistically validated. Linearity was observed in concentration range of 10-60μg/ml for Olopatadine. The accuracy of the method was evaluated by recovery studies and good recovery results were obtained between 98% to 100% and the relative standard deviation was found to be below 2% . A simple, accurate, sensitive and economical UV-spectrophotometric method for the estimation of Olopatadine pharmaceutical dosage form has been developed which can be employed in the industry for the routine analysis.

  16. Stability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage formDownload Article

    R.Srinivasan, J.Kamal Chandra, D.Rajesh Kumar, N.Dushyanth Kumar
    • Article Type: Research Article
    • View Abstract
    • Pages (445-452)
    • No of Download = 1598

    Abstract

    close

    A simple, accurate, rapid, precise and novel Reverse phase High Pressure liquid chromatographic method (RP-HPLC) has been developed and validated for determination of Azilsartan Medoxomil in bulk and its dosage form. The selected and optimized mobile phase was 0.05M potassium dihydrogen phosphate pH 4.0 : Acetonitrile in ratio of 60:40 and conditions were flow rate (1.0 ml/minute), wavelength (248 nm), Run time was 10 min. The retention time was found to be 3.8.Linearity and range was found to be 10-60 µg/ml. The proposed chromatographic conditions were found appropriate for the quantitative determination of the drugs.The method was validated for accuracy, precision, specificity, linearity, robustness, sensitivity, LOD and LOQ.it was also determined by uv-spectroscopy method. The proposed methods were successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that methods were specific, rapid, reliable, and reproducible.

  17. Simultaneous estimation of amitryptyline and chlordiazepoxide by RP-HPLC methodDownload Article

    R.Srinivasan, K.Lurdhu Mary, D.Rajesh Kumar, T.Parvathi
    • Article Type: Research Article
    • View Abstract
    • Pages (453-462)
    • No of Download = 642

    Abstract

    close

    A simple, accurate, rapid, precise and novel Reverse phase High Pressure liquid chromatographic method (RP-HPLC) has been developed and validated for simultaneous determination of Amitryptyline & Chlordiazepoxide. Amitriptyline and Chlordiazepoxide shows λmax at 230nm and 259 nm respectively. The selected and optimized mobile phase was mixed phosphate buffer (pH 3.0) and Acetonitrile were mixed in the ratio of 55:45 and conditions were flow rate (1.0 ml/minute), wavelength (252 nm), Run time was 12 min. The retention time were found to be 2.857 min and 5.667 min for Amitryptyline & Chlordiazepoxide respectively. Linearity and range was found to be 25-150 µg/ml for Amitryptyline and 10-60 µg/ml for Chlordiazepoxide. The proposed chromatographic conditions were found appropriate for the quantitative determination of the drugs. The method was validated for accuracy, precision, specificity, linearity, robustness, sensitivity, LOD and LOQ. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible.

  18. Development of validated RP-HPLC methods for quantification of donepezil and ranolzine from the in-house formulationsDownload Article

    R.Srinivasan, J.Kamal Chandra, D.Rajesh Kumar, D.Phanil Kumar
    • Article Type: Research Article
    • View Abstract
    • Pages (463-473)
    • No of Download = 475

    Abstract

    close

    A simple, accurate, rapid, precise and novel Reverse phase High Pressure liquid chromatographic method (RP-HPLC) has been developed and validated for simultaneous determination of Donepezil & Ranolzine from the in-house formulations. Chromatographic separation for Donepezil was performed on Agilient Zorbax C18 (150x 4.6 mm,) 5µm at a wavelength of 220 nm using a isocratic program for 10 min, by using mobile phase of OPA buffer solution and Methanol in the ratio of 60:40v/v pH 3.0. Donepezil obeys linearity in the range of 2 to 20µg/ml. The retention time was found to be 6.184 min. Chromatographic separation for Ranolzine was performed on Phenomenex Zorbax C18 (250x 4.6 mm,) 5µm at a wavelength of 220 nm using a isocratic program for 15 min, by using mobile phase of Potassium Phosphate buffer solution and Methanol in the ratio of 35:65v/v pH 7.0. Ranolzine shows linearity in the range of 25 to175µg/ml. The retention time was found to be 10.454 min. The proposed chromatographic conditions were found appropriate for the quantitative determination of the drugs. The method was validated for accuracy, precision, specificity, linearity, robustness, sensitivity, LOD and LOQ. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible.

  19. Compatibility and stability studies of levadopa, carbidopa, entacapone and natural bioenhancer mixtureDownload Article

    Rajkumar Dhawan, Dr.S.Ravi, Dr.T.Subburaju.
    • Article Type: Research Article
    • View Abstract
    • Pages (474-481)
    • No of Download = 1495

    Abstract

    close

    Levodopa, Carbidopa and Entacapone are used for the treatment of Parkinsonism disease. Piperine is an alkaloid from Piper species is reported to enhance the oral bioavailability of co-administered drugs. Piperine can improve the bioavailability of Levodopa in solid oral dosage forms. The present study was aimed to added Piperine natural bio-enhancer as a formulation additive in oral formulations of Levodopa, Carbidopa and Entacapone. The physical mixture of Levodopa, Carbidopa, Entacapone and Piperine in the ratio of 1:1 and the mixture were stored up to four weeks at 40°C/75%RH. In that samples are analyzed by validated Liquid chromatography techniques. The stability of the mixture was assessed by the use of ICH guidelines. Statistical experimental designs enable the user to obtain the maximum amount of information, i.e., the degradation effects of additives (Piperine) and their interactions on the stability of the drug substance. The percentage impurities of Levodopa, Carbidopa and Entacapone in Piperine after four weeks at 40°C/75%RH and are same like Initial results. The above studies have proved that Piperine can be used as a formulation additive in oral formulations of Levodopa, Carbidopa and Entacapone.

  20. Method development and validation for the simultaneous estimation of ambroxol Hcl and loratadine in a pharmaceutical formulation by RP-HPLC methodDownload Article

    Dondeti mogli reddy, Putchakayala Purna Chandra rao, D.Ramachandran*
    • Article Type: Research Article
    • View Abstract
    • Pages (482-491)
    • No of Download = 1025

    Abstract

    close

    An isocratic Simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of Ambroxol HCl and Loratadine in tablet dosage form. Quantification was achieved by using a reversed-phase C18 column (INERTSIL Column , 5µ, 250 mm × 4.6 mm) at ambient temperature with mobile phase consisting of Phosphate Buffer buffer (30mM) pH: 4.5: Acetonitrile : Methanol (30: 50:20). The flow rate was 1.0 ml/min. Measurements were made at a wavelength of 220nm. The average retention time for Ambroxol HCl and Loratadine were found to be 2.450 min and 4.367. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 72-168 µg/ml for Ambroxol HCl and 6 to 14 µg/ml for Loratadine. All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of Ambroxol HCl and Loratadine in tablet dosage form.

  21. A new reverse phase HPLC method for quick quantificationof all four isomers of natural vitamin-E oil in a single analysisDownload Article

    N.K.Sarathchandraprakash, Nagaraja.P.E., Prasanna Kumar T.P., Krishan Manral
    • Article Type: Research Article
    • View Abstract
    • Pages (492-499)
    • No of Download = 994

    Abstract

    close

    A simple and fast analytical method for quantification of natural vitamin E isomers by reverse phase High Performance Liquid Chromatography within short time. This method is developed to quantify in four natural isomers of vitamin- E, namely alpha, beta, gamma and delta tocopherol in fine formulations. The four isomers elution was carried out by using simple short column (100 X 4.6 mm) were used. And even mobile phase has used only Acetonitrile and water. The Limit of detection was achieved of isomers are very lower levels like 3 ppm. The method is capable to quantify 10 ppm to 100 % in a sample. The method is reliable robust, accurate and linear.

  22. Formulation and evaluation of combination porous tablet containing naproxen sodium as immediate release and sumatriptan succinate as sustained releaseDownload Article

    K.Anil Babu, V.Narasimha Rao, P.Dinesh Kumar, Anil Babu G, T.Krishna Sindhuri.
    • Article Type: Research Article
    • View Abstract
    • Pages (500-510)
    • No of Download = 782

    Abstract

    close

    Migraine is a chronic disorder and characterized by splitting headaches. A combination of sumatriptan succinate and naproxen sodium was found to be effective. Time to reach Cmax and biological half-life of both drugs are different and hence optimum levels of both drugs cannot be maintained simultaneously in the blood when these drugs administered orally in the form of conventional tablet. Therefore the object of present study was formulation development in vitro evaluation of porous combination tablet dosage form containing Naproxen sodium as immediate release in 45 min and sumatriptan succinate as sustained release to maintain optimum plasma levels of both drugs at a time and for a prolonged period of 12hrs. And here using sublimating agents camphor, and superdisintegrants like SSG for immediate release and HPMC K100M polymer to sustain the drug release.

  23. Influence of ethylcellulose on release of lamivudine from HPMC K4M transdermal patchesDownload Article

    Prakash Katakam, Narasimha Rao Rama
    • Article Type: Research Article
    • View Abstract
    • Pages (511-517)
    • No of Download = 634

    Abstract

    close

    The purpose of the present research was to formulate HPMC K4M based lamivudine transdermal patches and to study the influence of ethyl cellulose on its characteristics. TDDS formulations were prepared and characterized for the tensile strength, percentage elongation, water vapour transmission studies (WVT), water vapour absorption studies (WVA), FTIR, SEM, and in vitro drug release kinetics. The prepared patches showed good elasticity. The IR spectral studies showed that the drug remained in its normal form without undergoing any interaction with the polymers. The optimized formula based on drug release (F5) showed drug dissolution of 98.72% after 14 h.

  24. Analytical hierarchy process: A multi-criteria decision making approach for nanoparticles preparation methodDownload Article

    S. Mohan, L. Nandhakumar
    • Article Type: Research Article
    • View Abstract
    • Pages (518-523)
    • No of Download = 365

    Abstract

    close

    In our present work an analytical tool has been used to prioritize the suitable method used for the fabrication of nanoparticles containing few bioflavonoids. Nevertheless, quite a lot of preparation methods are available for the manufacturing of nanoparticles and it is very essential to select one such method, possessing the potential for successful fabrication of nanoparticles containing one or more bioflavonoids. Otherwise the usage of such analytical tool, in appropriate selection of methods is very vital to curb, the unnecessary time consumption of trying out at all methods available, which in turn escalates time and material resources. That has been the reason, here for employing Analytical Hierarchy Process for the selection of suitable fabrication methods for the nanoparticles. The Analytical Hierarchy Process was designed with the primary goal in the first stage (Goal-1) and second stage (Criterion-10) with various factors which influences the preparation method and thirdly with all sort of preparation methods (Sub criterion-10). A pair-wise comparison method has been performed between the ten criterions with each of ten sub-criterions the points were allotted using Saaty’s scale ranks ranging 1 to 9. The experimentation results revealed that nanoprecipitation method, which has gained a maximum priority points as compared with other methods. Hence, the nanoprecipitation method has been selected as the suitable nanoparticles preparation method for the fabrication of nanoparticles containing one or more bioflavonoids. At this juncture, it has also been observed as the Analytical Hierarchy Process method selection tool’s utilization for the method selection of nanoparticles.

  25. Hot melt extrusion technique and its pharmaceutical applications: A reviewDownload Article

    Sandhya Pamu , C. V. S. Subrahmanyam and K. S. K. Rao Patnaik
    • Article Type: Review Article
    • View Abstract
    • Pages (524-530)
    • No of Download = 341

    Abstract

    close

    Significance of hot-melt extrusion techniques for pharmaceutical applications is growing rapidly over the last three decades. Industrial malleability has allowed hot-melt extrusion (HME) to gain wide acceptance and has already established its importance in manufacturing operations and pharmaceutical research developments. Hot-melt extrusion techniques provide time controlled, modified, extended, and targeted drug delivery resulting in improved bioavailability as well as taste masking of bitter active pharmaceutical ingredients (APIs). HME offers several advantages over traditional pharmaceutical processing techniques including the absence of solvents, few processing steps, continuous operation, and the possibility of the formation of solid dispersions and improved bioavailability. Hot-melt extrusion techniques are pragmatic in the manufacture of a variety of dosage forms and formulations such as granules, pellets, tablets, suppositories, implants, stents, transdermal systems and ophthalmic inserts.

Sign in

Registered and Approved by National Science Library (NSL),National Institute of Science Communication And Information Resources(NISCAIR),
Council of Scientific and Industrial Research,New Delhi, India"