IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

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  1. Purification and characterization of L-asparginase producing bacteria MNTG-7Download Article

    M. Sunitha
    • Article Type: Research Article
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    • Pages (1-8)
    • No of Download = 461

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    Several techniques have been described for recovery and purification of L-asparaginase from different sources. The present study was undertaken to recover and purify L-asparaginase from mutated MNTG-7 cell line using three-step procedure involving ammonium sulphate precipitation, ion exchange and gel filtration chromatography followed by the characterization of the purified enzyme. Protein molecular mass markers, DEAE cellulose and Sephadex G-200 matrices, Agarose, acrylamide, bis-acrylamide, sodium dodecyl sulfate (SDS), TEMED, ammonium persulphate and bovine serum albumin were used in the purification process.

  2. A novel method for evaluation of 2-ethylhexanol in octyl stearate by using gas chromatography techniqueDownload Article

    N.K.Sarthchandra Prakash, Jampala Balaji, Krishan Manaral.
    • Article Type: Research Article
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    • Pages (9-15)
    • No of Download = 1170

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    A new innovative method developed for determination of 2-Ethylhexanol in Octyl Stearate by gas chromatography using capillary column and flame ionization detection. This method may also be applicable for determination of 2-Ethylhexanol in cosmetic products. The determination of 2-Ethylhexanol was developed first time in Octyl Stearate. This method was validated as per ICH guidelines. This method was simple, specific, precise, linear, accurate, robust and ruggedness for analysis of 2-ethylhexanol in Octyl Stearate.

  3. Development and validation of uv spectroscopy method for simvastatin in pH 6.8 phosphate bufferDownload Article

    S.Prasanthi, Dr.A.Rajendra Prasad, Y.Ganesh Kumar, Dr.K.Shantha Kumari
    • Article Type: Research Article
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    • Pages (16-20)
    • No of Download = 824

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    The aim of present work is to develop and validate simple, sensitive and specific Spectrophotometric method for the determination of simvastatin, a hypolipidemic drug in pure form and in pharmaceutical formulations.UV-Spectrophotometric method, which is based on measurement of absorption of U.V. The maximum wavelength in solvent system employed for determination of simvastatin was estimated at 233 nm in pH 6.8 phosphate buffer. The linearity range was found to be 0.01- 0.08 µg/mL(R2=0.999). The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. The optimum conditions for analysis of the drug were established. The drug obeyed the Beer’s law and showed good correlation. Beer’s law was obeyed in concentration range 0.01-0.08µg/ mL The method was found to be simple, accurate, precise, economical and robust. This method has been statistically validated and is found to be precise and accurate.

  4. Synthesis of novel β-carboline derivatives with potential antimicrobial and anti helminthic activitiesDownload Article

    G. Swetha, M. Prathyusha, Santhosh pawar V. B. Snigdha.
    • Article Type: Research Article
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    • Pages (21-29)
    • No of Download = 420

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    Synthesis of novel heterocyclic compounds containing the β-carboline moiety, which have a valuable biological activities, has been achieved through the nucleophilic substitution of tryptamine to N’-(2-(1H indo-3yl)ethyl) ethane1,2 diamine (I) followed by the cyclocondensation to the corresponding β-carboline derivatives (IV-IX). Isolated yields in the range of 50-80% were obtained. The structures of the newly synthesized compounds were elucidated by spectroscopic data. All the compounds were screened for the antibacterial and antihelminthic activities using streptomycin and albendazole as standard drugs.

  5. Chemical investigation of semi purified shorea robusta gum resin by using GCMSDownload Article

    D.Vishakante Gowda , Krishan Manral , Babu UV, Sarathchandraprakash, Jampala Balaji , Dr.K.Suresh Babu, S.Anubala.
    • Article Type: Research Article
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    • Pages (30-34)
    • No of Download = 874

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    The chemical investigation was done on Semi purified Shorea robusta gum resin (SG) to identify the chemical moieties. N-hexane fraction Shorea robusta gum, fractions were done by using column chromatography. The fractionation done by changing the polarities of n-Hexane and chloroform. All the fractions were collected carefully and solvents were removed by using Buchi Rotavapor. The collected fractions were subjected for GCMS evaluation. This study reveals the probable chemical components which were presenting in each and every fractions based on mass spectrums of GCMS. In the semi fractions of gum resin of Shorea robusta, the 100% hexane fraction was shown good emulsifying property. Based on the GCMS data, the probability of the molecular formulas of the components in 100% hexane fraction were C9H12, C13H28, C14H30, C10H22, C11H24, C12H26, C8H18, C9H19I, C8H16O, C6H14, C15H18O2, C9H14O2, C15H24, C16H34, C17H36, C14H30O3S, C14H31BO, C15H26O. This study gave the information about the probability of molecular formulas and its molecular structures of the components present in the semi fractions of gum resin of Shorea robusta based on GCMS data.

  6. Prescribing pattern of anti-hypertensive drugs: A prospective studyDownload Article

    Nisha Rani S S, Nelta S Tharakan, Gomathi Swaminathan*, Sattanathan .K, Shanmuga Sunadaram .R, Sambathkumar .R
    • Article Type: Research Article
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    • Pages (35-40)
    • No of Download = 638

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    The sources of drug utilization data vary from country to country depending on the level of sophistication of record keeping, data collection, analysis and reporting and the operational considerations of the health care system. So in most part of our country the prescribing pattern of antihypertensive drugs by different physicians is not in compliance with that of the standard guidelines. The purpose of this study was to evaluate the prescribing pattern of antihypertensive drugs and to determine the type of drugs commonly prescribed i.e. either monotherapy or combination drugs. This observational prospective study was conducted in erode for a time period of 4 months. The maximum percentage of male and females with hypertension was found at the age group of 50-60 years. As monotherapy ACE-inhibitors (34%) were the most commonly prescribed antihypertensive followed by calcium channel blockers (18%) and beta blocker (12%). Among combination therapy often 2 drug combinations were prescribed, the most common combination was ACE-inh + CCB (8%), followed by beta-blocker + CCB (6%). Hence we concluded that underutilization of diuretics and inadequate oral instructions by clinical pharmacist are found to be the limitations of the present prescribing pattern and hence, an intervention study is needed to improve the current prescribing practice in clinical use of hypertension management.

  7. Formulation and evaluation of valsartan pulsincap drug delivery systemDownload Article

    Anil Babu G, V.Vau Naik, Ankarao A, K.Anil Babu, P.V.A.Neelima
    • Article Type: Research Article
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    • Pages (41-47)
    • No of Download = 1386

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    The purpose of study was to formulate and evaluate Pulsincap drug delivery system of Valsartan for time release. Several functions such as Blood pressure (BP), heart rate, stroke volume, cardiac output, blood flow of the cardiovascular system are subject to circadian rhythms. For instance, capillary resistance and vascular reactivity are higher in the morning and decrease later in the day. Formulation with (programmable delivery) PDDS make it possible to delivery drug at specific time in chronopharmacokinetic studies. Valsartan prepared with a view to release the Valsartan around 5am with a lag time of 8hr after administration. The basic design consists of an insoluble hard gelatin capsule body filled with physical mixture of Valsartan granules and sealed with HPMC K100. We found that the type and amount of polymers influenced the drug release. Valsartan granules are prepared with different ratios of polymers like HPMC and/or EC by geometric mixing method. The lag time was dependent on the compassion of these polymers. Granules are filled in the formaldehyde treated insoluble capsule body and plugged with the HPMCK100. The finished dosage forms were subjected to various QC tests like uniformity of weight, drug content and in-vitro release. Promising results indicated Val F7 shows 99.58% of drug released with 8 hrs lag time. Thus this approach can provide a useful means for timed release of Valsartan and may be helpful for patients with morning surg.

  8. Concept of avartana W.S.R. to sneh kalpanaDownload Article

    Ved bhushan Sharma, Parul Sharma
    • Article Type: Research Article
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    • Pages (48-50)
    • No of Download = 1180

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    In Ayurvedic literature, various methods are described to potentiate the efficacy of a drug or Ayurvedic formulation. e.g. Bhawna, Ghana, Raskriya, etc. Avartana ia a kind of “Sanskar” which means Gunanteradhan i.e. assimilation of newer properties in a product/ formulation. In our classical texts reference of avartana came in Carak Samhita in the context of sneha as Anu tail, Aamlakayadi ghrit, Bhallatak tail and in Sushrut samhita with reference of Shatpaki & shastra paki tail etc. In reference of Sneha kalpana , the avartana helps to potentiate the properties of the sneha by adding prescribed quantity of ingredients. It helps in drug absorption and delayed excretion on administration. Here the concept of avartana, its method and advantages are discussed in vivid way.

  9. Formulation and evaluation of pulsatile drug delivery system using meloxicamDownload Article

    Sadaf muzaffar, Syed abdul azeez basha , Umm-e-hani, Mohd munawar ali tauqeer.
    • Article Type: Research Article
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    • Pages (51-59)
    • No of Download = 1199

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    In the current research work, meloxicam is taken as a model drug. Meloxicam is non steroidal anti inflammatory drug which is used in the treatment of rheumatoid arthritis in children. This research work deals with the preparation of eroding or soluble barrier layer surrounding the core tablet and the drug release is time dependent. The system releases the drug after a predetermined lag time of 8 h and thus the dosage forms can be taken at bedtime so that the content will be released in the morning hours, when the symptoms are more prominent. The optimized form of MELOXICAM with superdisintegrants like Xanthum gum and Guar gum in concentration (0.3-0.1g) may be attributed to rapid release of tablet. It was observed that disintegration time of tablet decrease with increased in concentration of these super disintegrants. The hardness of the pressed coated tablet was observed as 6.8 (Kg/cm2) whereas friability was less than 1% which indicated that tablet had good mechanical resistance. Drug content was found to be high (>98.14%) and uniform in all tablet formulations and % drug release was found to be 99.65% after 8 hours. The order of drug release from matrix system was described by using zero order kinetics. On the basis of these evaluation parameters it was found that P3F7 shows consistent release of drug after the predetermined lag time and therefore it was optimized as a promising approach of meloxicam as Pulsatile Drug Delivery System.

  10. A review on mouth dissolving tabletsDownload Article

    Manoranjan Sahu, Smitapadma Mohanty, Asish Dev
    • Article Type: Review Article
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    • Pages (60-67)
    • No of Download = 646

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    Now a day’s oral route of drug delivery considered as most convenient and economical method of drug delivery system. But for pediatric, geriatric, and bedridden patients it is difficult to swallow solid dosage form like tablets and capsules and may feel difficulty to take liquid dosage forms because of hand tremor. An active patient who is busy and traveling and may not access to water it’s a problem to administer a drug. By formulating mouth dissolving drug delivery system (MDDDS) all these drawbacks can be overcome. It has the advantage that it can avoid fast pass metabolism too. Mouth dissolving tablets are designed to be dissolved on the tongue rather than swallowed whole. So it will release the drug as soon as it will come in contact with saliva. This review article focused on various techniques involved in manufacturing of mouth dissolving tablets (MDTs) ranged from patented to non patented technologies. Characteristics of MDTs, advantages, disadvantages, factors responsible for sublingual absorption, challenges in formulating mouth dissolving tablets are discussed. This article demonstrate various evaluation tests of mouth dissolving tablets like weight variation, crushing strength, measurement of tablet porosity, friability, wetting time, in-vitro drug release, disintegrating test, modified disintegration test, water absorption ratio and stability studies.

  11. Development of RP-HPLC method for simultaneous estimation of ezetimibe and simvastatin in tablet formulationDownload Article

    Esmail O. Elakesh
    • Article Type: Research Article
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    • Pages (68-74)
    • No of Download = 828

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    A new, accurate, precise and robust HPLC method was developed and validated for the determination of Ezetimibe and Simvastatin in tablet dosage form. The chromatographic separation was achieved on an X-Terra C18 (150mm x 4.6mm, 3.5 µm) stationary phase maintained at ambient temperature with a mobile phase combination of 0.1% TEA and Acetonitrile (30:70) at a flow rate of 1.4 mL/min, and the detection was carried out by using UV detector at 238 nm. The total run time was 8 min. The retention time of Ezetimibe and Simvastatin were found to be 1.721 min. and 4.618 min. respectively. The performance of the method was validated according to the present ICH guidelines.

  12. Simultaneous HPLC method development and validation of moxifloxacin hydrochloride and bromofenac sodium in pharmaceutical formulationDownload Article

    Shine Sudev, Vishnumanikandan N, Sapna Shrikumar, Ajmal Shareef K, Najuma salim
    • Article Type: Research Article
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    • Pages (75-82)
    • No of Download = 936

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    This paper deals with the simultaneous reversed – phase HPLC method development for the for the simultaneous determination of Moxifloxacin Hydrochloride and Bromofenac Sodium in Pharmaceutical preparation. The chromatographic separation was carried out using a STD Hyber C18 column, 150× 4.6mm i.d, 5µ particle size in isocratic mode with flow rate of 1mLmin-1 and the detection was carried out by Photo diode array detector at 280nm. The mobile phases consist of 0.01N Sodium Dihydrogen Ortho Phosphate buffer (pH 4 ±0.5) and Acetonitrile in the ratio of 65:35 (v/v). The total chromatographic analysis per sample was approximately 6 mints. Retention times for Moxifloxacin Hydrochloride and Bromofenac Sodium were found to be 2.26 and 5.72 min respectively. A linear response curve was observed over the concentration range of 25-150µgmL-1 and 4.5-27µgmL-1 for Moxifloxacin Hydrochloride and Bromofenac Sodium. The method was statistically validated as per ICH guidelines and % RSD was found to be less than 2 indicating high degree of accuracy and precision of the proposed HPLC method. Hence the proposed method can be applied for the quantitative analysis of Moxema and Bromday eye drops.

  13. A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical dosage formDownload Article

    Jampala Balaji, K.Aruna, N.V.S.Naidu
    • Article Type: Research Article
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    • Pages (83-87)
    • No of Download = 975

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    A simple reverse phase high performance liquid chromatography method developed for quantification of sumatriptan succinate in bulk and pharmaceutical dosage form. This method achieved by using isocratic elution with mixed phosphate buffer (pH: 6.8) and acetonitrile mobile phase at 70:30 ratio, Hypersil BDS C18 (150 X 4.6mm, 5µm) column at temperature 30ºC, flow rate 1.0mL/min and 228 nm UV with PDA detector. This method validated as per ICH guidelines. This method was simple, specific, precise, linear, accurate, robust and ruggedness for analysis of sumatriptan succinate.

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