IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

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  1. Formulation and evaluation of controlled porosity osmotic pump for oral delivery of valsartanDownload Article

    P.Vinay Kumar, K.Ashwin Kumar, D.Karthikeyan
    • Article Type: Research Article
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    • Pages (597-604)
    • No of Download = 179

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    The aim of the present study was to design a controlled porosity osmotic pump tablet of Valsartan. The controlled porosity osmotic pump tablet contains opdry CA as semipermeable coat form contact with water, dissolve, resulting in an in situ formation of a microporous structure. The dosage regimen of Valsartan is 80-350mg tablet 2-3times a day. The plasma of life ranges from 6-7hrs.Hence Valsartan was chosen as a model drug with an aim to develop a controlled release system for a period of 24hrs.The effect of different formulation variables. It was found that drug release was directly related to the amount of osmogent and level of pore former. The optimized formulation was subjected to stability studies as per international conference on harmonization (ICH) guidelines and formulation was stable after 3 months study.

  2. ICH guidelines of manufacturing and quality assurance of drugs and cosmeticsDownload Article

    R.Suthakaran *, Raju.Manda, K.Sunil Kumar, M.Ramesh and K.Aparna
    • Article Type: Research Article
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    • Pages (605-617)
    • No of Download = 88

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    The key events in the development of the generic drug industry after the Hatch-Waxman Act of 1984 are systematically reviewed, including the process of approval for generic drugs, bioequivalence issues including “switchability”, bioequivalence for complicated dosage forms, patent extension, generic drug safety, generic substitution and low-cost generics. The backlog in generic review, generic drug user fees, and “quality by design” for generic drugs is also discussed. The evolution of the US generic drug industry after the Hatch-Waxman Act in 1984 has afforded several lessons of great benefit to other countries wishing to establish or re-establish a domestic generic drug industry.

  3. Formulation and evaluation of sulfasalazine tablets for colon targeting using 33 response surface methodDownload Article

    Mohd. Rawoof, K. Rajnarayana, M. Ajitha
    • Article Type: Research Article
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    • Pages (618-634)
    • No of Download = 267

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    The aim of the study was to develop colon targeted tablets of Sulfasalazine (SSZ) by wet granulation method using 33 Response surface method with Design of experiment software and Eudragit RS 100, Eudragit RL 100-55, Ethyl cellulose and PVP K-30 as pH dependent polymers. All the formulations (F1 to F27) were evaluated for the physicochemical parameters and were subjected to in vitro drug release studies. The amount of Sulfasalazine released from tablets at different time intervals was estimated by UV spectrophotometer. The formulation F17 shown 98.21±1.15 of Sulfasalazine after 24h, where as marketed product drug release was 96.21±1.87 after 1 h. The results of the study showed that formulation F17 is the best formulation on the basis of drug release and other evaluation parameters and the pH dependent tablet system is a promising vehicle for preventing rapid hydrolysis in gastric environment and improving oral bioavailability of Sulfasalazine for the treatment of disease at colon region.

  4. Regulatory affairs on health CanadaDownload Article

    R.Suthakaran, P. Sambasivarao, M. Ramesh, V.Padma, Raju.Manda
    • Article Type: Research Article
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    • Pages (635-644)
    • No of Download = 93

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    Regulatory affairs (RA), also called government affairs, is a profession within regulated industries, such as pharmaceuticals, medical devices, energy, banking, telecom etc. Regulatory affairs also has a very specific meaning within the healthcare industries (pharmaceuticals, medical devices, biologics and functional foods). Regulatory affairs (medical affairs) professionals (aka regulatory professionals) usually have responsibility for the following general areas: Ensuring that their companies comply with all of the regulations and laws pertaining to their business. Working with federal, state, and local regulatory agencies and personnel on specific issues affecting their business. i.e. working with such agencies as the Food and Drug Administration or European Medicines Agency (pharmaceuticals and medical devices); The Department of Energy; or the Securities and Exchange Commission (banking).Advising their companies on the regulatory aspects and climate that would affect proposed activities. i.e. describing the "regulatory climate" around issues such as the promotion of prescription drugs and Sarbanes-Oxley compliance.

  5. Formulation and evaluation of poly herbal hand wash gel containing essential oilsDownload Article

    A. Mounika, Vijayanand P, V. Jyothi
    • Article Type: Research Article
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    • Pages (645-653)
    • No of Download = 457

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    The present research was aimed to evaluate the antibacterial efficacy of various herbal oils such as Cinnamon, eucalyptus, orange, peppermint; clove, geranium and rosemary oils found that cinnamon, geranium and peppermint showed better antibacterial, antimicrobial activities. The research was carried out to formulate and evaluate the poly herbal gels. Hand wash gel containing Cinnamon and geranium oil. The anti-microbial activity of the formulated herbal hand wash gel was tested against Escherichia coli and Staphylococcus aureus by pour plate techniques and the results obtained were compared with commercial antibacterial standards. Also the efficiency was checked by using the hand wash gel on volunteers.

  6. The effect of superdisintegrants on the dissolution of atenolol fast dissolving tabletsDownload Article

    P.Vinay Kumar, G. Sushma, Ganesh Kumar Gudas
    • Article Type: Research Article
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    • Pages (654-659)
    • No of Download = 101

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    In the present study an attempt has been made to prepare fast dissolving tablets of atenolol, by using different superdisintegrants with enhanced disintegration & dissolution rate. Atenolol is a potent Antihypertensive agent. It competes with sympathomimetic neuro transmitters such as catacholamines such as catecholamines for binding at beta (1) - adrenergic receptors in the heart and vascular smooth muscle, inhibiting sympathetic stimulation. Fast dissolving tablets of atenolol were prepared by using different natural superdisintegrants like fenugreek seed mucilage, Modified Gum karaya and Locust bean gum by direct compression. The precompression blend was tested for angle of repose, bulk density, tapped density, compressibility index and Hausner’s ratio. The tablets were evaluated for weight variation, hardness, friability, disintegration time (1 min), dissolution rate, content uniformity, and were found to be within standard limit. It was concluded that the fast dissolving tablets with proper hardness, rapidly disintegrating with enhanced dissolution can be made using selected superdisintegrants. Among the different formulations of atenolol prepared and studied, the formulation F12 containing Locust bean gum was found to be the fast dissolving formulation.

  7. Development and characterization of fast dissolving tablets of doxazosin mesylate by using novel coprocessed super disintegrating technologyDownload Article

    P.Vinay Kumar, Ch. Venkatesh, K. Ashwin Kumar
    • Article Type: Research Article
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    • Pages (660-665)
    • No of Download = 91

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    In the present work, an attempt has been made to develop fast disintegrating tablets of Doxazosin mesylate. Novel method of co processed super disintegrates technology was employed to formulate the tablets. All the formulations were prepared by direct compression method. The blend of all the formulations showed good flow properties such as angle of repose, bulk density, tapped density. The prepared tablets were shown good post compression parameters and they passed all the quality control evaluation parameters as per I.P limits. Among all the formulations F14 formulation showed maximum drug release i.e., 99.3% in 4 min hence it is considered as optimized formulation. The F14 formulation contains CP5 as super disintegrate in the concentration of 30 mg. (CP 5 contains Vivasole and polyplasdone XL in 3:1 ratio).

  8. Formulation and evaluation of metformin hydrochloride extended release tabletsDownload Article

    P.Vinay Kumar, P.Raju
    • Article Type: Research Article
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    • Pages (666-672)
    • No of Download = 104

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    Metformin hydrochloride has relatively short plasma half-life, low absolute bioavailability. The need for the administration two to three times a day when larger doses are required can decrease patient compliance. Sustained release formulation that would maintain plasma level for 8-12 h might be sufficient for daily dosing of metformin. Sustained release products are needed for metformin to prolong its duration of action and to improve patient compliances. The overall objective of this study was to develop an oral sustained release metformin hydrochloride tablet by using polymers like Methocel K100M CR, Polyox WSR 303, Ethocel 100 cps as rate controlling factor. The tablets were prepared by wet granulation method. The precompression blend was tested for angle of repose, bulk density, tapped density, compressibility index and Hausner’s ratio. The tablets were evaluated for weight variation, hardness, friability, dissolution rate, content uniformity, and were found to be within standard limit. The invitro dissolution study was carried out using USP II apparatus method and the data was analysed using zero order, first order, Higuchi, Korsmeyer and Hixson-Crowell equations. The drug release study revealed that F5 shows sustain the drug release for more than 12 h. Kinetic modeling of in vitro dissolution profiles revealed the drug release mechanism ranges from diffusion controlled or Fickian transport to anomalous type or non-Fickian transport.

  9. Formulation & evaluation of colon targeted drug delivery system of omeprazoleDownload Article

    P.Vinay Kumar, Ganesh kumar Gudas
    • Article Type: Research Article
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    • Pages (673-678)
    • No of Download = 125

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    The purpose of the present study was to formulate colon delivery tablets of Omeprazole, an Anti-ulcer agents. Omeprazole tablets were prepared by direct compression method employing rate controlling the synthetic polymer Eudragit RSPO, Eudragit RLPO The Prepared granules were subjected for pre compressional and drug excipient compatibility studies and tablets were evaluated for post compressional parameters such as weight variation, hardness, friability, drug content, and Invitro dissolution studies. It was concluded that the out of all the formulations F12 was the best formulation as the extent of drug release was found to be 96 % upto 24 hrs and the kinetics of drug release was follows first order kinetics with the ‘n’ value of more than 0.5 indicates that non-fickian mechanism. % Cumulative drug release from all the prepared formulation was found to be in following order F12>F11>F10>F9>F8>F7>F6>F5>F4>F3>F2>F1 The kinetics of optimized formulation F12 was found to be follows first order kinetics and the ‘n’ is more than 0.5 indicates that it follows non-fickian mechanism. Endured super case II release, during the dissolution study, which indicated that polymer relaxation, had a significant role in the drug release mechanism. Drug release mechanism was case II non-Fickian (anomalous) release (0.5≤ n ≤ 0.89).

  10. Clinical approach to nadivrana W.S.R to pilonidal sinusDownload Article

    Dr.Amareshappa, Dr.Manoj Kumar, Dr.Shailaja S V
    • Article Type: Research Article
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    • Pages (679-682)
    • No of Download = 112

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    The term Nadi vrana implies a tube-like structure and Nadi vrana means sinus. Nadi vrana is a secondary condition of improper drainage of abscess, negligence of foreign body in wound, tuberculosis. Pakvashopha upeksha, Anuchita paatanakarma, Mithya aahar&vihara, Shalya are the main cause for nadi vrana. Sushruta classified nadivrana into vataja, pittaja, kaphaja, sannipataja, dwandwaja, agantuja whereas vagbhata classified the same except dwandwaja. Sadhyasadhyata asTridosha is Asadhya & vataja, pittaja, kaphaja, dwandaja and shalyaja are saadhya. Varti prayoga, ksharasutra & shastra karma are the treatment of choice in nadivrana. Nadivrana can be compaired to sinus and shalyaja / agantuja nadivrana as pilonidal sinus. Sinus is a blind track leading from surface down into the tissue and lined by granulation tissue. It is mostly formed secondary to abscess and most of the time results into fistula. Treatment includes proper Antibiotics, adequate rest, adequate excision, adequate drainage & treat the secondary causes. Pilonidal sinus is a common disease of the natal cleft in the sacrococcygeal region with weak hair accumulation occurring in the hair follicles, which can be chronic and undergo acute exacerbation. The patient presents with the complaints, which are characteristics of inflammation-a painful swelling, redness, local temperature raised or with a sinus discharge. There are 2 types as primary- opens in midline, secondary opens lateral to midline. The Causes for reoccurrence are improper removal of track, entry of new tuft of hairs, breakage of scar, irregular shaving of hair, improper hygiene. Regular shaving of the hair in natal area, proper perianal hygiene, proper drainage of the pus, avoid the causative factors are the preventive measures for pilonidal sinus. Treatment includes surgical management, it is of 2 types. Close surgery includes, excision with primary closure and flap closure. Open surgery includes conventional wide excision and laser pilonidoplasty

  11. Clinical approach to vriddhi roga W.S.R to mootravriddhiDownload Article

    Dr.Amareshappa, Dr.Manoj Kumar, Dr.Shailaja S V
    • Article Type: Research Article
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    • Pages (683-687)
    • No of Download = 118

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    Vriddhi indicates pathological increase in dosha & dhatu. Doshavriddhikaraahara -vihara is the main cause for vriddhi. Acharya Susrutha has classified vriddhiroga into 7 types. Vatadosha is the main component in the development of vriddhiroga. In Vriddhiroga Charaka mentioned Virechanadi chikitsa can be adopted in aamavastha of doshajavriddhiroga and Vranachikitsa in Pakvavastha. In case of medovriddhi, kaphajavriddhi, mootravriddhi , Patana, Seevana followed by Vranaropana chikitsa should be adopted. Mootravriddhi is the type vriddhi is caused due to vataprakopa which leads to accumulation of mootra in vrushana. Ambupoornadhrutivat kshoba, Mrudu, Saruk, Mootrakruchcha, Chalayanaphalakosha are the signs and symptoms of mootravriddhi. It can be compared to vaginal hydrocele. The defect in obliteration of the vitello-intestinal duct is the main cause for hydrocele. Clinically it is classified into Congenital and Acquired, congenital hydrocele common at early age of life due to patent Vitello-intestinal duct.The acquired variety type is classified into Vaginal hydrocele, Infantile hydrocelebilocular hydrocele, encysted hydrocele, hydrocele of the hernia sac, hydrocele of the canal of the nuck, the other secondary hydrocele is Post herniorrhaphy hydrocele, Filarial hydrocele, Chylocele. Blood routine, urine routine, USG scrotum, aspiration are the main diagnostic criteria. The differential diagnosis of hydrocele is inguinal hernia, epididymal cyst, spermatocele, testicular tumour, infection, pyocele, haematocele, atropy of testis, infertility, hernia of hydrocele sac. Surgery is the choice of treatment; it includes subtotal excision, jobouleys operation, evacuation & evertion. Even acharyas also mentioned shastra karma for management of mootravriddhi.

  12. Antioxidant activities of crude extracts of pulsatilla nigricans stockDownload Article

    G Sunil, Erram Nagendram, Meesa Rajendar
    • Article Type: Research Article
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    • Pages (688-691)
    • No of Download = 110

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    The present study investigates the in-vitro radical scavenging activity of different extracts obtained from the dry arial parts of Pulsatilla Nigricans by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and nitric oxide assay. The methanolic extract of Pulsatilla Nigricans showed signifcant DPPH radical scavenging activity at IC50 of 25.67 μg/ml and low radical scavenging activity at 747.15 μg/ml with methanolic extract in nitric oxide assay.

  13. Formulation and in vitro evaluation of liquisolid compacts of telmisartanDownload Article

    G sunil, E.Swapna priya, Meesa Rajendar
    • Article Type: Research Article
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    • Pages (692-696)
    • No of Download = 88

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    Telmisartan is Angiotensin receptor blocker, ARB used for the treatment of the Hypertension. This drug is poorly soluble in water that causes slow dissolution rate of the drug and also slow absorption which eventually leads to the inadequate and low oral bioavailability[4] i.e., 43%. To overcome this problem the drug is formulated with the most novel technology the Liquisolid compaction. In this technology, the insoluble drug is made dissolved in the suitable non-volatile water miscible solvent to form the drug solution which is then compressed directly into the Liquisolid tablets by the addition of suitable carrier and coating materials along with the lubricant, glidant, and disintegrants. In this study, Neusilin, the widely accepted multifunctional excipient is used in the formulation of telmisartan compacts .Neusilin is the synthethic granule of magnesium alumina metasilicate.

  14. Development and validation of the quantitative analysis of lisinopril dihydrate in tablet formulation by fourier transform infrared spectroscopyDownload Article

    Shital Dange, Shailesh Wadher, Tukaram Kalyankar, M. Krishna Yadav
    • Article Type: Research Article
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    • Pages (697-704)
    • No of Download = 118

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    A simple, precise, non accurate, rapid, inexpensive, ecofriendly and reproducible FTIR spectrophotometric method for quantitative determination of lisinoprildihydrate in tablet formulation was developed and validated. This method concerned with measurement of absorbance measurements of bands corresponding to OH stretch centered by 3625-3490 cm-1. Analytical method validation was carried out to study the parameters as linearity, repeatability, precision and accuracy. The linearity range was found to be 0.3-1.8 % w/w (regression equation: y=0.300x-0.030, r2 = 0.994). The data show good precision results of this method, since the RSD values observed less than 2 %. The proposed FTIR method was successfully applied to the assay of lisinoprildihydrate in bulk drugs and tablet formulation.

  15. Development and validation of the quantitative analysis of amlodipine besylate in tablet formulation by fourier transform infrared spectroscopyDownload Article

    Shital Dange, Tukaram Kalyankar, Shailesh Wadher, Sai Priyanka
    • Article Type: Research Article
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    • Pages (705-716)
    • No of Download = 89

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    A simple, precise, non destructive, rapid, and inexpensive, ecofriendly and reproducible FTIR spectrophotometric method for quantitative determination of amlodipine besylate in tablet formulation was developed and validated. This method concerned with absorbance measurements of bands corresponding to c=o stretch centered by 1731-1687 cm-1. Analytical method validation was carried out to study the parameters as linearity, repeatability, precision, accuracy as well as photo degradation study. The linearity range was found to be 0.3-1.8 % w/w (regression equation: Y=0.585x-0.044, r2 = 0.994). The data show good precision results of this method, since the RSD values observed less than 2 % as per ICH limits. The proposed FTIR method was successfully applied to the assay of amlodipine besylate in tablet formulation.

  16. Method development and validation for the simultaneous estimation of ambroxol HCL and levofloxacin by using RP HPLC methodDownload Article

    B.Jyothsna, V.Swapna
    • Article Type: Research Article
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    • Pages (717-732)
    • No of Download = 76

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    A new, simple, precise, accurate and reproducible RP-HPLC method for Simultaneous estimation of bulk and pharmaceutical formulations. Separation of Ambroxol HCL and levofloxacin was successfully achieved by Inertsil C18, 250 X4.6, 5µm or equivalent in an isocratic mode utilizing 0.1% OPA: methanol (60:40) at a flow rate of 1mL/min and eluate was monitored at 238nm, with a retention time of 2.173 and 4.344 minutes for Ambroxol HCL and levofloxacin respectively. The method was validated and there response was found to be linear in the drug concentration range of 50µg/ml to150 µg/ml for Ambroxol HCL and 50µg/ml to150 µg/ml for Levofloxacin. The values of the correlation coefficient were found to0.999for Ambroxol HCL and 1for levofloxacin respectively. The LOD and LOQ for Ambroxol HCL were found to be 0.035and 0.116 respectively. The LOD and LOQ for Levofloxacin were found to be 0.1884 and 0.6281 respectively. This method was found to be good percentage recovery for Ambroxol HCL and Levofloxacin were found to be 99.1% and 99.7% respectively indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample so, the method specifically determines the analyte in the sample without interference from excipients of tablet dosage forms. The method was extensively validated according to ICH guidelines for Linearity, Accuracy, Precision, Specificity and Robustness

  17. Formulation development and characterization of pioglitazone transdermal drug delivery systemDownload Article

    Chandra Sekhar. Pabbathi, Balamini. Vattepu, Vinod.Veerla, Rajamani. Allakonda
    • Article Type: Research Article
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    • Pages (733-742)
    • No of Download = 81

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    The main objective of the present study was to formulate and evaluate matrix type Pioglitazone transdermal patches and to determine the drug release. Firstly, characterization of the drug was done by performing FTIR compatibility studies and found that there was no interaction between the drug and polymers under study. Formulations (F1 to F6) were prepared using different ratios of HPMC E15 and Eudragit L 100 and penetration enhancer DMSO was incorporated to the above formulations (F7 to F12).These formulations were evaluated for weight variation, thickness variation, folding endurance, %moisture content, %moisture absorption studies, drug content, mechanical properties and exvivo permeation studies. In formulations F1 to F12, the drug permeation was maximum for F4 and F10 (ratio 10:2 HPMC E15: Eudragit L100). Among these, formulations F10 is exhibited the required flux.

  18. Development and evaluation of controlled porosity osmotic pump tablet of losartan potassiumDownload Article

    Kausar Sulthana, G.Ganesh Kumar
    • Article Type: Research Article
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    • Pages (743-753)
    • No of Download = 76

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    The aim of the present work was to prepare and evaluate controlled porosity osmotic pump tablets of Losartan potassium to prolong the release of drug oral administration. Losartan potassium is an anti-hypertensive drug and it acts as an angiotension II receptor antagonist. The tablets were prepared by the wet granulation method using mannitol as an osmogen and polymers like polyox N-80, polyox N-205 at different concentrations. The tablets were coated with opadry CA upon contact with water it results in an in situ formation of a micro porous structure. Total twelve (F1-F12) were formulated and the tablets were evaluated for various parameters such as compatibility studies, drug content, weight variation, hardness, thickness, friability, in vitro drug release studies and release rate kinetics. The drug-polymer interaction was also studied by conducting FTIR. The in vitro release kinetics studies reveal that all formulations fits well with Zero order, followed by Korsmeyer-Peppas and the mechanism of drug release follows super case II transport. After analysis of different evaluation parameters and drug release kinetics, formulation code F9 was selected as a promising formulation for delivery of Losartan potassium as a controlled porosity osmotic pump tablet with 99.82 % in vitro drug release at 24th hour. The stability studies were carried out at 40°C/75% RH for 90 days. There was no significant change in the physical property during the study period.

  19. Formulation and evaluation of floating tablets of esomeprazoleDownload Article

    D.Sumith Reddy, Ganesh Kumar Gudas
    • Article Type: Research Article
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    • Pages (754-761)
    • No of Download = 79

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    The present study outlines a systematic approach for designing and development of Esomeprazole floating tablets to enhance the bioavailability and therapeutic efficacy of the drug. Floating tablets of Esomeprazole have shown sustained release thereby proper duration of action at a particular site and are designed to prolong the gastric residence time after oral administration. Different formulations were formulated by using wet granulation technique. A floating drug delivery system (FDDS) was developed by using sodium bicarbonate as gas-forming agent and chitosan, compritol 888ato, HPMC K15 M as polymers. The prepared tablets were evaluated in terms of their physical characteristics, precompression parameters, in vitro release and buoyancy lag time. The results of the in vitro release studies showed that the optimized formulation (F6) could sustain drug release for 12 hrs by using HPMC K15M in the concentration of 60 mg. The in vitro drug release followed zero order kinetics. The stability of optimized formulation (F6) was known by performing stability studies for three months at accelerated condition of 400C±75%RH on optimized formulation. The F6 formulation was found to be stable with no change.

  20. Review on therapeutic uses of NETIDownload Article

    Dr.Vidya M.Barki, Dr.Sumithra.T.Gowda, Dr.Sumithra.T.Gowda
    • Article Type: Review Article
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    • Pages (762-766)
    • No of Download = 96

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    Yoga is a part of general search for better health, vitality and awareness that is rapidly becoming an important part of one’s culture. Internal cleansing is probably the first step towards a healthier life, which includes not only special detoxification measures of a clinical nature but daily hygienic practices that everyone can do. The Yoga tradition offers an important tool of self-healing, a special device called ‘Neti pot’ and a process of cleansing the nostrils or nasal irrigation that it is used for. The use of Neti procedure is not only common to Yoga practitioners but is extended in to the entire natural health community along with the use of herbs, massage, and other alternative therapies. Physicians suggest the Neti pot as a part of right lifestyle considerations both for the prevention of disease and for the attainment of optimal energy. The neti pot is an important tool of nasya therapies. Other nasya methods involve placing medicated oils into the nose with eyedroppers, snuffing powdered herbs, massage of the nasal region and other forms of steam therapy and massage to the head. The use of the neti pot can be enhanced by these other nasya methods and can be used along with them. With proper guidance from health care professional, one can practice Neti procedure on daily purpose to clear nasal passage.

  21. Method development and validation of simultaneous estimation of miconazole and metronidazole in tablet dosage form RP-HPLC methodDownload Article

    B.Jyothsna, V. Swapna
    • Article Type: Research Article
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    • Pages (767-779)
    • No of Download = 14

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    A new simple, accurate, precise isocratic high performance liquid chromatographic (HPLC) method was developed and validated for the determination of miconazole and metronidazole in tablet formulation. The optimized conditions comprise of column C18,150mmx4.6, 5 m particle size with a flow rate of 1.0 mL/min, Mobile Phase: 0.1%OPA: Methanol (75:25) mixture was used at a detection wavelength 268 nm. Retention times of miconazole and metronidazole were found to be 2.87min, and 3.75 min respectively. The method was validated in terms of linearity, accuracy, precision, specificity, LOD and LOQ. The LOD values of miconazole and metronidazole were found to be 0.01 and 0.014 µg/mL, LOQ’s were found to be 0.03 and 0.04µg/mL respectively. Method is linear in the range of 50 to 150%. This new method was successfully developed and validated as per ICH guidelines, can be utilized for the quantitative estimation of miconazole and metronidazole pharmaceutical dosage forms.

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