IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

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  1. Reverse phase-high performance liquid chromatography method estimation of levocetirizine and montelukast in tablet dosage formsDownload Article

    Mala Swapna, Srikanth Choudary Pallothu
    • Article Type: Research Article
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    • Pages (152-157)
    • No of Download = 101

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    In the current study a simple, accurate and precise reverse phase liquid chromatographic method has been developed for simultaneous estimation of Levocetirizine Hydrochloride and Montelukast Sodium from tablet dosage form. The method was developed using HPLC series Compact System Consisting of Inertsil-C18 ODS column, using a mixture of 0.05 (M) Potassium Dihydrogen Phosphate Buffer and Methanol : buffer (80:20) as mobile phase in an isocratic elution mode at a flow rate of 1.0 ml/min, at 30ºC with a load of 10µl. The detection was carried out at 225 nm. The retention time of Levocetirizine and Montelukast were found to be around 2.8 min and 43.9 min respectively. The method was validated with respect to linearity, robustness, precision and accuracy and was successfully applied for the simultaneous quantitative determination of Levocetirizine Hydrochloride and Montelukast Sodium from the tablets.

  2. Development and validation of RP-HPLC method for ritonavir in bulk and pharamceutical dosage formsDownload Article

    CH. Shankar, D.V.R.N. Bhikshapathi, R. Suthakaran, CH. Pavani Padmaja, Amtul Wahab
    • Article Type: Research Article
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    • Pages (158-164)
    • No of Download = 60

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    A reverse phase high-performance liquid chromatographic method was developed and validated for the quantitative determination of Ritonavir. Chromatographic method was carried out by isocratic technique using C18 Column, Phenomenex (250 x 4.6 mm, 5 μ), with mobile phase mixture of acetonitrile: methanol 70:30(V/V) and at a flow rate of 1 mL/min. The retention time obtained for Ritonavir at 3.52 min at 239 nm wavelength. The different analytical parameters such as linearity, precision, accuracy, specificity and robustness, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization guidelines. The linearity of the calibration curves for Ritonavir in the desiredconcentration range is good (r2>0.9). The recovery of the method was between 98% and 102% for Ritonavir. Hence the proposed method is highly sensitive, precise and accurate and it successfully applied for the estimation of API content in the commercial formulations of ritonavir.

  3. A quantitative RP-HPLC method development and validation for sofosbuvir in bulk and tablet dosage formDownload Article

    Darshan Bhatt, Padmini Thatavarthi, B. Rajkamal
    • Article Type: Research Article
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    • Pages (165-171)
    • No of Download = 67

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    A new isocratic simple and rapid reverse phase high performance liquid chromatographic method was developed and successively validated for the estimation of Sofosbuvir. In this newly developed method, chromatographic separation of Sofosbuvir was achieved on a Phenomenex C18- column (250 × 4.6) mm within a short runtime of 6 min using mobile phase containing 0.1% Formic acid in water (pH at 2.3) and Acetonitrile in the ratio of 50:50% v/v. Sofosbuvir was estimated with UV detection at 262 nm and it was found to be eluted at 2.983 min. The above mentioned method was validated as per International Conference on Harmonization (ICH) guidelines with respect to accuracy, precision, linearity, limit of detection (LOD) and limit of quantitation (LOQ) and robustness. The method was found specific for Sofosbuvir and linear (r2 =0.9994) over concentrations ranging from 20 to 100 μg/mL. The method was found statically accurate (mean recovery = 99.46%), precise with both intra-day and inter-day relative standard deviation (RSD) values < 1.0% and robust. The obtained results concluded that the proposed RP-HPLC method is convenient, reliable and useful in routine analysis for estimation of Sofosbuvir in its bulk form and dosage form.

  4. Analytical method development and validation of pantaprazole in tablet dosage form by using UV spectroscopic method as per ICH guidelinesDownload Article

    V.Shirisha, Mahendar Boddupally, K.Rajeswar Dutt, M.bhavana, B.Mounika, CH.Asha, Sk.Basheer
    • Article Type: Research Article
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    • Pages (172-178)
    • No of Download = 53

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    A simple, accurate, precise and economic spectrophotometric method has been developed for the determination of Pantaprazole in their pharmaceutical dosage form. Pantaprazole showed maximum absorbance at 290 nm with Methanol as solvent. Beer’s law was obeyed in the concentration range 10-60 μg/ml with regression coefficient of 0.999. The concentration of active component were then determined from the calibration curve obtained by measuring the amplitude at 290 nm for Pantaprazole. Accuracy and precision of the developed methods have been tested in addition recovery studies have been carried out in order to confirm their accuracy. The method was validated in terms of linearity, precision, accuracy (100.1-100.13%w/w) and specificity. This method is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.

  5. HPTLC fingerprint profile of selected anti-diabetic plants monitored with reference to flavonoidsDownload Article

    M.Dhanamani*, T.K.Ravi
    • Article Type: Research Article
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    • Pages (179-190)
    • No of Download = 38

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    The main objective of the present study was to evaluate the phytoconstituents and finger print profile in the various extracts of two important anti-diabetic species namely Terminalia catappa and Cressa cretica. The plant species were extracted with different solvents such as petroleum ether, chloroform, ethyl acetate and methanol in the increasing order of polarity. Preliminary phytochemical screening of the various extracts of these two species revealed the presence of flavonoids, phenols, tannins and saponins in the methanolic extract. HPTLC methods for the separation of the active constituents in extracts have been developed. HPTLC finger print for different extracts revealed the presence of peaks with Rf values in the range of 0.01 to 0.99. The bands of purple and light yellowish orange colour revealed the presence of flavonoids and phenolic compounds. Routine quality control of these species could be carried out using this hptlc method and it serves in qualitative analysis, quantitative estimation and was appropriate for standardization of the extract.

  6. Synthesis and Biological evaluation of some new Heterocyclic compoundsDownload Article

    Surendra Singh, Y.RajendraPrasad, Ellamma.M
    • Article Type: Research Article
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    • Pages (191-195)
    • No of Download = 31

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    2-pyrazolines (B1PY1-B10PY10) derivatives were prepared by treating chalcones (B1-B15) with phenyl hydrazine in presence of pyridine and ethanol. All the synthesized compounds were depicted by means of IR, 1HNMR spectral data and microanalyses. The prepared compounds were studied for analgesic effect, some of them were found to have significant activity, when compared to standard drugs.

  7. Comprehensive Study on Assessment of Knowledge, Attitude and Practice of Adverse Drug Reaction Monitoring and Reporting among Nurses in a Tertiary Care HospitalDownload Article

    K.Jeeva Balaji, S.Safna, A.Shama Anjum, M.Suresh, A.Tamil Selvan
    • Article Type: Research Article
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    • Pages (196-200)
    • No of Download = 34

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    Pharmacovigilance is the science and activities relating to the detection, assessment, understanding and prevention of adverse effects or any other drug related problems. Present pilot study deals with the assessment of ADR monitoring and reporting among nurses in a tertiary care hospital. The results reveal that nurses have sufficient knowledge on ADR monitoring and reporting. Regular teaching and training of the nurses on various aspects of adverse event monitoring and reporting may improve further in good clinical practices and adhere good compliances on patient health care.

  8. Formulation and invitro evaluation of albendazole nanosuspensionsDownload Article

    Mothkuri Sathish Kumar
    • Article Type: Research Article
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    • Pages (201-205)
    • No of Download = 82

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    Albendazole is an BCS class II drug is a broad-spectrum anthelmintic structurally related to mebendazole that is effective against many diseases. It is successfully used in experimental and clinical chemotherapy of different intestinal and systemic parasitosis. Its efficacy is often limited by poor intestinal absorption due mainly to its low aqueous solubility. Efficacy of albendazole can be enhanced by improving its dissolution. Reduction in particle size to Nano scale can help in dissolution improvement by increase in surface area. Due to its high half-life it can be formulated as a Nano suspension. Nano suspension is a novel technique used to increase the solubility of the drug prepared by solvent evaporation method. The Nano suspensions are novel promising target and controlled released dosage form which is gaining importance because of ease of manufacturing and diversified applications. The present trend of pharmaceutical research lies in the usage of biodegradable polymer because of its availability and low toxicity. Nanosuspension containing drug was prepared by solvent evaporation method by using combinations of Tween 80, Polaxomer, PVP-K90, PVA, and Acetone and quantity sufficient water). Estimation of Albendazole was carried out spectrophotometrically at 246nm. The Nano suspension were evaluated for parameters such as drug content uniformity, scanning electron microscopy, particle size analysis, zeta potential, in-vitro release, short-term stability, drug excipient interactions (FTIR). The stability data was also subjected to statistical analysis. The drug release from the Nanosuspension was explained by the using mathematical model equations such as zero order, first order, and equation methods. Based on the regression values it was concluded that the optimized formulation F9 follows Zero order kinetics.

  9. Formulation and evaluation of alginate microspheres of pramipexole HCLDownload Article

    B. Sandhyarani, G. Arjun, K. Subbarao, G. Ganesh Kumar, D.V.R.N. Bhikshapathi
    • Article Type: Research Article
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    • Pages (206-217)
    • No of Download = 30

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    The present work was designed to formulate and evaluate the Pramipexole microspheres. Drugs with short half-life frequent administration of dosage form is required, so the possible way by which this can be overcome by preparing sustained release microspheres of Pramipexole which can also improve patient compliance. The alginate microspheres were prepared by ionotropic gelation technique. Sodium alginate and chitosan were used as polymers, calcium chloride as cross-linking agent, and Olibanum gum is also used. Microspheres were characterized for the Micromeritic properties, incorporation efficiency, Swelling Index, SEM analysis, FTIR, and in vitro release studies. Among all formulations S14 was optimized on the basis of different evaluation parameters. The % yield of S14 formulation was found to be 96.41 %, entrapment efficiency and swelling index of S14 formulation was 95.26%, 97% respectively. The Cumulative % drug release of S14 formulation was 97.19±5.10% in 12h when compared with marketed product 90.45±5.10 in 12hrs. SEM studies showed the particles were in spherical shape. The method developed in the present study can be effectively utilized to achieve the formulation with desired release characteristics in the effective management of Parkinson’s disease.

  10. MASS GAINER POWDER; Helps to increase muscle mass, reduce body fat increases strength & Keeping muscles healthy and optimising their repair to maximise performanceDownload Article

    Govind Shukla, Neha S.Giri, D. Sruthi Rao, C.J. Sampath Kumar
    • Article Type: Research Article
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    • Pages (218-225)
    • No of Download = 29

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    Keeping muscles healthy and optimising their repair after training is extremely important in sport to maximise performance. For many sports, it is also desirable to increase muscle mass, reduce body fat overall and increase strength where necessary. This review summarises the current available scientific literature regarding the effect of MASS GAINER POWDER; Helps to increase muscle mass, reduce body fat increases strength & Keeping muscles healthy and optimising their repair to maximise performance.

  11. Comparative evaluation of antioxidant, hypoglycemic and hypolipidemic potentials of Black tea from three major tea growing zones of IndiaDownload Article

    Shanta K Adiki, Koushik Bhandari, Prakash Katakam, Gargi Saha, Baishakhi De
    • Article Type: Research Article
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    • Pages (226-232)
    • No of Download = 32

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    Tea is a very popular commercial crop and India is the world’s largest consumer of tea in the world and the second largest producer of tea. Black tea is mostly preferred in Indian context and its multifaceted health benefits are being largely explored. This research article made a comparative study of antioxidant, hypoglycemic and hypolipidemic effect of Assam, Darjeeling and Nilgiri varieties of black tea. Research results have shown that Assam variety of tea has the highest antioxidant, hypoglycemic and hypolipidemic potentials followed by Nilgiri and Darjeeling variety.

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