IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

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  1. Simultaneous determination and validation of third generation antiviral drugs by RP-HPLC methodDownload Article

    L.Memthoibi Devi, Dr.T.Rama Mohan Reddy, Dr.K.Abbulu
    • Article Type: Research Article
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    • Pages (01-08)
    • No of Download = 15

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    A simple reverse phase high pressure liquid chromatography (RP-HPLC) method has been developed and validated for simultaneous determination of Sofosbuvir, Velpatasvir and Voxilaprevir in tablet dosage forms. The drugs were separated on Discovery C18 (150 x 4.6 mm, 5) column using 0.1% othophosphoric acid and Acetonitrile ratio (60:40%v/v) as the mobile phase at a buffer having pH 2.2. The mobile phase is pump into the column at flow rate of 1ml/min and column oven temperature is maintained at 30°C. The drugs were detected at a wavelength 220nm. The retention time for Sofosbuvir, Velpatasvir and Voxilaprevir were found to be 2.120min, 3.164 min and 3.800min. %RSD. The percentage recovery drugs were found to be in range of 99.08%, 98.97% and 99.29% respectively. The Limit of detection, Limit of quantification were 0.21ppm, 0.62ppm, 0.03ppm, 0.09ppm and 0.27ppm, 0.81ppm respectively. Regression equation of concentration over there peak area were found to be Sofosbuvir was y =28579.x + 23225, Velpatasvire was y = 38719x +11703and of Voxilaprevir was y = 38712.x + 74459, Y is the peak area and X is the concentration of drug. The method is useful in the simultaneous determination of third generation antiviral drugs by RP-HPLC.

  2. Development and validation of stability indicating RP-HPLC method for the estimation of Elbasvir and Grazoprevir in bulk and pharmaceutical dosage formDownload Article

    K.Surayya Begum, Mrs. Nallakumar Ponnuswamy
    • Article Type: Research Article
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    • Pages (09-17)
    • No of Download = 12

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    A simple, Accurate, precise method was developed for the simultaneous estimation of the Elbasvir and Grazoprevir in tablet dosage form. Chromatogram was run through Denali C18 150 x 4.6 mm, 5. Mobile phase containing Buffer 0.1% OPA (2.8ph): Acetonitrile taken in the ratio 600:30 was pumped through column at a flow rate of 0.8 ml/min. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Elbasvir and Grazoprevir were found to be 2.143 min and 2.694 min respectively. The drug was stressed under alkaline, oxidative, thermal, photolytic degradation were analysed. The developed method was validated as per ICH guidelines The Accuracy, Linearity, Precision, and Robustness were within the acceptance limits .Hence this HPLC method was a stability indicating method can be used for routine stability analysis of the Elbasvir and Grazoprevir in Pharmaceutical dosage forms.

  3. Assay Method for Simultaneous Estimation of Epalrestat and Pregabalin in Pure and its Dosage form by RP-HPLCDownload Article

    N.Veena, Dr.T.Rama Mohan Reddy, Dr.K.Abbulu
    • Article Type: Research Article
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    • Pages (18-27)
    • No of Download = 13

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    In the proposed method, a new RP-HPLC method has been developed for simultaneous estimation of Epalrestat and Pregabalin in pure and its dosage form. The present method was a sensitive, precise, and accurate RP-HPLC method for analysis of Epalrestat and Pregabalin. To optimize the mobile phase, various combinations of buffer and organic solvents were used on Std BDS C18 column (4.6 x 150mm,5µm). Then the mobile phase containing a mixture 0.1% OPA: Acetonitrile (52:48) was selected at a flow rate of 1ml/min at a detector wavelength of 240 nm foretention time, baseline stability and minimum noise. The retention times of Epalrestat and pregabalin were found to be 2.930 min and 2.179 min respectively. The limit of detection and quantification of Epalrestat and Pregabalin were found to be 0.20 and 0.62; 0.18 and 0.56 respectively, which indicates the sensitivity of the method. The high percentage recovery indicates that the proposed method is highly accurate. No interfering peaks were found in the chromatogram indicating that excipients used in formulations didn’t interfere with the estimation of drugs by the proposed HPLC method.

  4. Stability Indicating Method Development and Validation for the Simultaneous Determination of Escitalopram and L-methyl Folate in Bulk and Pharmaceutical Dosage Form by RP-HPLCDownload Article

    Vijaya Laxmi, Mrs Sri Vidya, Dr.K.Abbulu
    • Article Type: Research Article
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    • Pages (28-37)
    • No of Download = 12

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    In the presented work the stability indicating RP-HPLC method was developed for the simultaneous estimation of the L-methyl folate and Escitalopram in bulk and pharmaceutical dosage form .The L methyl folate and Escitalopram were analysed through Std BDS C18 150 x 4.6 mm, 5m.Using the mobile phase consisting of (0.01N KH2PO4 buffer: Acetonitrile)(50:50,v/v) with a flow rate of 0.9ml/min. The wavelength was selected at 230 nm using uv detection. The L Methyl folate and Escitalopram were eluted at 2.232min and 3.279min respectively. The drug was stressed under alkaline, oxidative, thermal, photolytic degradation were analysed. The developed method was validated as per ICH guidelines The Accuracy, Linearity, Precision, and Robustness were within the acceptance limits .Hence this HPLC method was a stability indicating method can be used for routine stability analysis of the Escitalopram and l methyl folate in Pharmaceutical dosage forms.

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