IJPAR | International Journal of Pharmacy and Analytical Research

International Journal of Pharmacy and Analytical Research

ISSN: 2320_2831

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  1. Simultaneous determination and validation of third generation antiviral drugs by RP-HPLC methodDownload Article

    L.Memthoibi Devi, Dr.T.Rama Mohan Reddy, Dr.K.Abbulu
    • Article Type: Research Article
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    • Pages (01-08)
    • No of Download = 81

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    A simple reverse phase high pressure liquid chromatography (RP-HPLC) method has been developed and validated for simultaneous determination of Sofosbuvir, Velpatasvir and Voxilaprevir in tablet dosage forms. The drugs were separated on Discovery C18 (150 x 4.6 mm, 5) column using 0.1% othophosphoric acid and Acetonitrile ratio (60:40%v/v) as the mobile phase at a buffer having pH 2.2. The mobile phase is pump into the column at flow rate of 1ml/min and column oven temperature is maintained at 30°C. The drugs were detected at a wavelength 220nm. The retention time for Sofosbuvir, Velpatasvir and Voxilaprevir were found to be 2.120min, 3.164 min and 3.800min. %RSD. The percentage recovery drugs were found to be in range of 99.08%, 98.97% and 99.29% respectively. The Limit of detection, Limit of quantification were 0.21ppm, 0.62ppm, 0.03ppm, 0.09ppm and 0.27ppm, 0.81ppm respectively. Regression equation of concentration over there peak area were found to be Sofosbuvir was y =28579.x + 23225, Velpatasvire was y = 38719x +11703and of Voxilaprevir was y = 38712.x + 74459, Y is the peak area and X is the concentration of drug. The method is useful in the simultaneous determination of third generation antiviral drugs by RP-HPLC.

  2. Development and validation of stability indicating RP-HPLC method for the estimation of Elbasvir and Grazoprevir in bulk and pharmaceutical dosage formDownload Article

    K.Surayya Begum, Mrs. Nallakumar Ponnuswamy
    • Article Type: Research Article
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    • Pages (09-17)
    • No of Download = 60

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    A simple, Accurate, precise method was developed for the simultaneous estimation of the Elbasvir and Grazoprevir in tablet dosage form. Chromatogram was run through Denali C18 150 x 4.6 mm, 5. Mobile phase containing Buffer 0.1% OPA (2.8ph): Acetonitrile taken in the ratio 600:30 was pumped through column at a flow rate of 0.8 ml/min. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Elbasvir and Grazoprevir were found to be 2.143 min and 2.694 min respectively. The drug was stressed under alkaline, oxidative, thermal, photolytic degradation were analysed. The developed method was validated as per ICH guidelines The Accuracy, Linearity, Precision, and Robustness were within the acceptance limits .Hence this HPLC method was a stability indicating method can be used for routine stability analysis of the Elbasvir and Grazoprevir in Pharmaceutical dosage forms.

  3. Assay Method for Simultaneous Estimation of Epalrestat and Pregabalin in Pure and its Dosage form by RP-HPLCDownload Article

    N.Veena, Dr.T.Rama Mohan Reddy, Dr.K.Abbulu
    • Article Type: Research Article
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    • Pages (18-27)
    • No of Download = 50

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    In the proposed method, a new RP-HPLC method has been developed for simultaneous estimation of Epalrestat and Pregabalin in pure and its dosage form. The present method was a sensitive, precise, and accurate RP-HPLC method for analysis of Epalrestat and Pregabalin. To optimize the mobile phase, various combinations of buffer and organic solvents were used on Std BDS C18 column (4.6 x 150mm,5µm). Then the mobile phase containing a mixture 0.1% OPA: Acetonitrile (52:48) was selected at a flow rate of 1ml/min at a detector wavelength of 240 nm foretention time, baseline stability and minimum noise. The retention times of Epalrestat and pregabalin were found to be 2.930 min and 2.179 min respectively. The limit of detection and quantification of Epalrestat and Pregabalin were found to be 0.20 and 0.62; 0.18 and 0.56 respectively, which indicates the sensitivity of the method. The high percentage recovery indicates that the proposed method is highly accurate. No interfering peaks were found in the chromatogram indicating that excipients used in formulations didn’t interfere with the estimation of drugs by the proposed HPLC method.

  4. Stability Indicating Method Development and Validation for the Estimation of Escitalopram and L-Methylfolate in Bulk and Pharmaceutical Dosage Form by RP -HPLCDownload Article

    Vijaya Laxmi, Mrs.A.Srividya, Dr.K.Abbulu
    • Article Type: Research Article
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    • Pages (28-37)
    • No of Download = 48

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    In the presented work the stability indicating RP-HPLC method was developed for the simultaneous estimation of the L-methyl folate and Escitalopram in bulk and pharmaceutical dosage form .The L methyl folate and Escitalopram were analysed through Std BDS C18 150 x 4.6 mm, 5m.Using the mobile phase consisting of (0.01N KH2PO4 buffer: Acetonitrile)(50:50,v/v) with a flow rate of 0.9ml/min. The wavelength was selected at 230 nm using uv detection. The L Methyl folate and Escitalopram were eluted at 2.232min and 3.279min respectively. The drug was stressed under alkaline, oxidative, thermal, photolytic degradation were analysed. The developed method was validated as per ICH guidelines The Accuracy, Linearity, Precision, and Robustness were within the acceptance limits .Hence this HPLC method was a stability indicating method can be used for routine stability analysis of the Escitalopram and l methyl folate in Pharmaceutical dosage forms.

  5. Formulation and evalaution of chlorzoxazone transdermal emulgel by using natural penetration enhancerDownload Article

    A.Susmitha, Ganesh Kumar Gudas
    • Article Type: Research Article
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    • Pages (38-44)
    • No of Download = 39

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    Emulgel is one of the recent technologies in NDDS used for dual control release of emulsion and gel for topical use. Gel formulations generally provide faster drug release compared with conventional ointments and creams. Chlorzoxazone is a well-known therapeutic agent that is used mainly for its skeletal muscle relaxants. The aim and objective of the study is to formulate Chlorzoxazone emulgel for topical application. Emulgel of Chlorzoxazone, consist of Carbopol-940 or HPMC K4 as a gelling agents for gel formulation and tween 80, span 20, for emulsion formulation. Emulgel was formulated by emulsion incorporated in gel. Chlorzoxazone loaded emulgel was formulated by using o/w emulsion because of lower solubility in water. Lemon grass oil, Menthol was used as a penetration enhancer in emulgel formulation. Optimized formulation was evaluated for physical examination, swelling index, skin irritation study, extrudability study, drug content determination, spreadability, globule size determination and invitro drug release, rheological study. Optimized formulation shown drug release 98.8%. for 12 hrs.

  6. Investigation of phytochemicals & estimation of total phenolic and flavonoid content of myxopyrum smilacifolium (wall.) blume leavesDownload Article

    Litty Joseph, Anusha Shaji
    • Article Type: Research Article
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    • Pages (45-51)
    • No of Download = 42

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    Myxopyrum smilacifolium (Wall.) Blume is a large woody climbing shrub belonging to the family Oleaceae. It is used in the treatment of cough, asthma, neuropathy, rheumatism, cephalalgia and otopathy. The main objective of the study is to investigate the presence of various phytochemicals and estimate the total phenolic and flavonoid content in various fraction of Myxopyrum smilacifolium (Wall.) Blume leaves. The crude ethanol extract was subjected to fractionation using Petroleum ether, Chloroform and Ethyl acetate based on their polarity. The fractions were subjected to preliminary phytochemical analysis for the detection of various phytoconstituents. The total phenolic and flavonoid content in different fractions were estimated by Folin-Ciocalteau method and Aluminium chloride colorimetric assay respectively. The qualitative phytochemical analysis revealed the presence of carbohydrates, alkaloids, flavonoids and phenolics. Ethanol residue and petroleum ether fraction of M. smilacifolium was found to be rich in phenolics and flavonoids respectively.

  7. Formulation and evaluation of sustained release tablets of indinavir by using natural polymersDownload Article

    M.Aruna, D.Karthikeyan
    • Article Type: Research Article
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    • Pages (52-58)
    • No of Download = 28

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    The main objective of this research work was to formulate and evaluate of sustained release tablets of Indinavir by using natural polymers. It is having a short biological half-life (1.5 h) so it is considered as a suitable drug for the formulation of sustained release tablets to prolong its therapeutic action. Sustained release tablets were prepared by wet granulation technique, using synthetic and natural polymers at different ratios. Granules were prepared and evaluated for bulk density, tapped density, Hausner’s ratio, compressibility index. The Fourier-transform infrared spectra of the indinavir and different natural polymers alone show the compatibility of the drug with excipients. The physicochemical properties of tablets were found within the limits. The prepared tablets were evaluated for weight variation, thickness, hardness, % friability, % drug contents, and in vitro release. In vitro dissolution studies (USP dissolution rate test apparatus II, 50 rpm, 37°C ± 0.5°C) was carried out for the first 2 h in 0.1 N HCl (1.2 pH) and followed 6.8 phosphate buffer for 12 h as a dissolution medium. The optimized formulation F7 was shown maximum drug release 97.3±0.22% in 12 h of dissolution.

  8. Development and validation of UV spectroscopic method for simultaneou estimation of dapagliflozin and saxagliptin in synthetic mixtureDownload Article

    Mr.Mohd.Zameeruddin, Miss.Sandhya S. Bundel, Mr.Vishvanath B. Bharkad, Miss.Hajera N. Khan, Mr.Thoke Sandip T
    • Article Type: Research Article
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    • Pages (59-66)
    • No of Download = 29

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    Aim Simple, precise and accurate UV-Spectrophotometric Simultaneous Equation method for estimation of Dapagliflozin and Saxagliptin were developed and validated as per ICH guidelines. Experimental and Results The objective of the work is to develop UV spectroscopic method for simultaneous estimation of Dapagliflozin (DAPA) and Saxagliptin (SAXA). This Method involve solving of simultaneous equations based on measurement of absorbance at two wavelengths 223 nm and 212 nm. Both the drugs obey the Beer’s law in the concentration ranges 4-24 µg/mL and 5-50 µg/mL respectively. Results of the methods were validated statistically. Novel, simple, sensitive, rapid, accurate and economical Spectrophotometric methods have been developed for simultaneous estimation of Dapagliflozin and Saxagliptin .The method can be used to estimate the amount of Dapagliflozin and Saxagliptin in mixture containing Dapagliflozin and Saxagliptin. Conclusion The results of estimation and validation parameters like accuracy, precision, ruggedness, linearity and range were studied for all the developed methods and were found to be within limits. The proposed method can be adopted for routine quality control for estimation of drug in formulation.

  9. Formulation and characterisation of repaglinide buccal tabletsDownload Article

    Kuldeep Waidya, Tenali Janaki, Bali Reddy, Krishna Reddy, K.N.V. Rao, Rajeshwar Dutt
    • Article Type: Research Article
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    • Pages (67-80)
    • No of Download = 23

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    The objective of this study was to develop effective buccal tablets of Repaglinide. Tablets of Repaglinide were prepared by direct compression method using bioadhesive polymers like Chitosan, Guar gum, Xanthan gum. Buccal tablets were prepared by taking polymers in different ratios. Buccal tablets were evaluated by different parameters such as thickness, hardness, weight uniformity, content uniformity, surface pH, in-vitro drug release, ex vivo drug permeation, in vivo mucoadhesive performance studies. In vitro assembly was used to measure the bioadhesive strength of tablets with fresh porcine buccal mucosa as model tissue. The tablets were evaluated for in vitro release in pH 6.8 phosphate buffer for 8 hr in standard dissolution apparatus. In order to determine the mode of release, the data was subjected to Zero order, First order, Higuchi, Korsmeyer and Peppas diffusion model. The formulation F3 showed maximum drug release (89.06%) in 8 hrs. The optimised formulation F3 showed a surface pH of 6.18. This formulation was following Zero order mechanism with regression value of 0.981. FT-IR studies revealed the absence of any chemical interaction between drug and polymers used. Repaglinide buccal tablets for buccal delivery could be prepared with required in-vitro release properties.

  10. Formulation and characterization of RH-Vascular Endothelial Growth Factor loaded Solid lipid nanoparticles for chronic wounds healingDownload Article

    Mr.A.Sathyaraj, Dr.D.Ramashekar Reddy
    • Article Type: Research Article
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    • Pages (81-92)
    • No of Download = 22

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    Vascular endothelial growth factor (VEGF) is one of the most potent proangiogenic growth factors in the skin, and the amount of VEGF present in a wound can significantly impact healing. In this research study, rhVEGF (recombinant Vascular Endothelial Growth Factor) was epitomized solid lipid nanoparticles. In vitro tests were attempted in fibroblasts and keratinocytes to decide the bioactivity of the typified rh-VEGF and to gauge the cell take-up ability of the lipid nanoparticles, and their effectiveness was compared with that of a few intra-lesional organizations of a higher measurement of free rhVEGF and that of a solitary intra-lesional organization of rh-VEGF-loaded solid lipid nanoparticles created in our research facility. Mending was assessed as far as wound conclusion, recuperation of the incendiary stage, and re-epithelisation review.

  11. Proein and peptide drug delivery systemDownload Article

    B. Narasimha Rao, K. Ravindra Reddy, D. Anusha Reddy, S. Rahath Fathima, M. Surekha
    • Article Type: Research Article
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    • Pages (93-98)
    • No of Download = 19

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    In the last three decades therapeutic uses of peptides and proteins has risen in prominence as potential drug of future. Proteins are polymers consisting of amino acids covalently linked by peptide bonds. Peptides are small proteins composed of upto a few dozen amino acids proteins are rapidly degraded by digestive enzymes. Till recently injections (i.e., intra venous, intra muscular, subcutaneous route) remain the most common for administering these proteins and peptide drugs. The alternate routes that have been tried with varying degrees of success the oral, buccal, intranasal, pulmonary, transdermal, ocular and rectal. In this review the aim is to focus on the various approaches for delivery of peptide and protein drugs. With the discovery of insulin in 1922, identification and commercialization of potential protein and peptide drugs have been increased. Since then, research and development to improve the means of delivering protein therapeutics to patients has begun.

  12. Formulation Development and Evaluation of liquisolid compacts of Epelerenone HydrochlorideDownload Article

    G.Swetha, N. Vijay Kumar Ph.D
    • Article Type: Research Article
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    • Pages (99-110)
    • No of Download = 15

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    Eplerenone hydrochloride is a poorly water soluble drug, which is an aldosterone receptor antagonist used in the management of chronic heart failure. The rate of its oral absorption is often controlled by dissolution rate in the gastro intestinal tract. The aim of the present research work was to improve the solubility and dissolution properties of insoluble drug, eplerenone hydrochloride by liquisolid technique. Liquisolid compacts of eplerenone hydrochloride were formulated by dispersing the drug in various non volatile liquid vehicles. Several formulations were prepared using a new mathematical model to calculate the appropriate powder and liquid ingredients required to produce acceptably flowing and compressible mixtures. Avicel PH 102, Lactose monohydrate and Syloid 244 FP were used as carrier materials and Aerosil was used as coating material. The effect of different concentrations of drug on the dissolution rate was studied and it was observed that the liquisolid compacts containing 25%w/w of drug in solvent with lactose monohydrate and Aerosil in the ratio of 10:1 gave the maximum dissolution rate when compared with other formulations. Optimized formulation was compressed into tablets after addition of sodium starch glycolate as a disintegrant in a concentration of 5% of the total weight of tablet and evaluated. Enhanced drug release rates were observed when compared to pure drug and conventional tablet. This is due to increased wetting properties and surface of drug available for dissolution. It was further confirmed from the ex vivo studies that the liquisolid formulation has shown improved rate of permeation than the pure drug and conventional tablet. The crystalline state of eplerenone drug state was changed to amorphous state due to liquisolid formation and was confirmed by X-ray diffraction study. Fourier transform infrared spectroscopy results revealed that there was no interaction between drug and excipients.

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