Estimation cefixime and ornidazole simultaneous in tablet dosage form by RP-HPLC method

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Cefixime and Ornidazole in Tablet dosage form. Mobile phase containing Buffer and Degassed Methanol and Buffer in the ratio of 60:40 V/V, has been pumped through column at a flow rate of 1ml/min. Buffer used in this method was of  di-sodium hydrogen phosphate and potassium dihydrogen phosphate. Temperature was maintained at 30°C. Optimized wavelength for Cefixime and Ornidazole was 254 nm. Retention time of Cefixime and Ornidazole were found to be 2.1 min and 4.9 min. %RSD of the Cefixime and Ornidazole were and found to be 0.21 and 0.26 respectively. The percentage recovery was obtained as 99.81% and 99.76% for Cefixime and Ornidazole respectively. LOD, LOQ values are obtained from regression equations.