Comparison of analytical parameter of genetically transformed hairy roots of withania somnifera with normal roots
Keywords:Withania somnifera, Withanolide-D Authetification, Fingureprint, HPTLC profile, Standardization.
Withania somnifera, known as Ashwagandha, widely considered as Indian Ginseng, is a plant of repute in Indian system of traditional medicine. The present study was aimed to compare high performance thin layer chromatography(HPTLC) profile of methanol extract of hairy roots of Withania somnifera with normal roots extracts purchased from different places. The normal roots extracts were obtained from different places like Tulsi Amrit, Indore; Natural Remedies, Bangalore; Ansar Industries, Surat; Amsar Private Limited, Indore; Prashant Pharmaceuticals, Rajpipla. Transgenic hairy roots were induced in W. somnifera by infecting leaf explants with two wild type strain of Agrobacterium rhizogenes ATCC 15834 and MTCC 4364 using MS media. Chromatographic method was used for separation of withanol ide-D from extracts of roots. The W. somnifera hairy roots extract and normal root extracts purchased from different places and standard Withanolide-D sample were used in HPTLC using Solvent system: Toluene: Ethyl acetate: Formic acid (5:5:1) [V]. HPTLC profiling of extracts confirm about presence of Withanolide -D.HPTLC fingureprint profile scanned at 530nm for methanolic extract revealed with Rf value in range of 0.51 to 0.55. The hairy root extract of Withania somnifera showed almost similar rf (0.53) value and similar peak area (5934.27) when compared with the standard Withanolide-D. The HPTLC method for routine quality control and comparison of present species can be carried out by using method for extracts of plant which serve in qualitative, quantit ative and was appropriate for standardization of extracts.