New method develment for the estimation of neuroactive drug (pimavanserin) by RP-HPLC method

Abstract

RP-HPLC method has been developed & validated for the analysis of Pimavanserin API. The HPLC is the method of choice in the field of analytical chemistry since this method is specific, robust, linear, precise and accurate and the limit of detection is low and also it offers: Speed (many analysis can be accomplished in 20 min or less), Greater sensitivity (various detectors can be employed) Improved resolution (wide variety of stationary phases) Reusable columns (expensive columns but can be used for many analysis) Ideal for the substances of low viscosity Easy sample recovery, handling and maintenance. To develop a precise, linear, specific & suitable stability indicating RP-HPLC method for analysis of Pimavanserin, different chromatographic conditions were applied & the results observed in chapters. Isocratic elution is simple, requires only one pump & flat baseline separation for easy and reproducible results. So, it was preferred for the current study over gradient elution. In case of RP-HPLC various columns are available, but here Symmetry ODS RP C₁₈, 5mm, 15mm x 4.6mm i.d. column was preferred because using this column peak shape, resolution and absorbance were good. Mobile phase & diluent for preparation of various samples were finalized after studying the solubility of API in different solvents of our disposal (methanol, ethanol, water, DMF, DMSO).