Analytical Method Development For The Estimation Of Antihypertensive Drug (Olmesartan) By Rp-Hplc Method

Abstract

A sensitive and selective RP-HPLC method has been developed and validated for the determination of Olmesartan in pure form and Pharmaceutical dosage form. The Retention time of Olmesartan was found to be 3.544minutes. The Percentage Standard Deviation (%RSD) of the Olmesartan was and found to be 1.63%. The detector response was linear in the concentration range of 0-16µg/ml. The respective linear regression equation being Y= 58945.x + 9634 with R2 = 0.999. The percentage recovery values of Olmesartan in pharmaceutical dosage form were found to be within the limits. The limit of detection and the limit of quantification for Olmesartan were found to be 0.90µg/ml and 2.90µg/ml respectively. Further the proposed RP-HPLC method has excellent sensitivity, precision and reproducibility, accuracy. The result shows the developed method is yet another suitable method for assay, purity which can help in the analysis of Olmesartan in different marketed formulations. The developed method was validated as per ICH guidelines for accuracy calculated as % recovery was in the range of 98.0% to 102.0%. The statistical analysis of the data showed that the method is reproducible and selective for the estimation of Olmesartan in pure form and marketed Pharmaceutical dosage form during routine analysis. The results of the study showed that, the proposed RP-HPLC method was simple, rapid, precise, accurate which can be used for the routine determination of Olmesartan in pure form and marketed pharmaceutical dosage forms.