Analytical method development and validation for the simultaneous estimation of sulfadiazine and pyrimethamine by rp-hplc method

Authors

  • Dipendra Kumar Gupta M.Pharm Student, Aditya Bangalore Institute of Pharmacy education and Research, Kogilu, Yelahanka, Bangalore-64, India.
  • Paul Richards M Professor & HOD, Department of Pharmaceutical Analysis and QA, Aditya Bangalore Institute of Pharmacy Education and Research, Aditya Bangalore Institute of Pharmacy education and Research, Kogilu, Yelahanka, Bangalore-64, India.
  • Suchitra D Associate Professor, Dept of Pharmaceutical Chemistry, Aditya Bangalore Institute of Pharmacy education and Research, Kogilu,Yelahanka,Bangalore-64, India.
  • B A Vishawanath Principal And Chairman Aditya Bangalore Institute of Pharmacy education and Research, Kogilu, Yelahanka, Bangalore-64, India.

Keywords:

Zodiac silica C18 column, Sulfadiazine and Pyrimethamine, RP-HPLC

Abstract

Background

Estimation of Sulfadiazine and Pyrimethamine  is a combination drug of choice used to treat malaria who are living in or will be travelling to an area where there is chance of getting malaria.

Objective

The regimen above is in short supply and to evaluate efficacy of twice weekly maintenance therapy to prevent recurrent toxoplasmic encephalitis in patients with Acquired Immnuno deficiency syndrome.

Materials and methods

A  HPLC  (Alliance,Water2695)  with  UV/VIS  Detector/PDA  detector,  UV  (lab  India,UV  3000  series)  and Agilent  C18  250mm  ×  4.6mm  ×  5µm  column  was  used.  A  new  method  was  established  for  simultaneous estimation  of Sulfadiazine and  Pyrimethamine by RP-HPLC method.  The chromatographic conditions  were successfully developed for the separation of Sulfadiazine and Pyrimethamine by using Zodiac sil C18 column

 

(4.6×150mm)5µ,   flow   rate   was

 

1ml/min,   mobile   phase   ratio   was   (70:30   v/v)   methanol:   phosphate

 

buffer(KH2PO4 and K2HPO4) phosphate pH 3 (pH was adjusted with orthophosphoricacid),detection  wavelength was 240nm.

Results

The results were in good agreement with those obtained with official HPLC with absorption maximum of 240 nm by preparing mobile phase 70:30 methanol : phosphate buffer with flow rate 1 ml/min and it run for 10 minutes by selecting column Zodiac silca  RP C18 4.5×100 mm 3.0 µm of ambient temperature .All the results obtained with good precise ,accurate and robustness as per ICH guidelines.

Conclusion

It  can be concluded that the proposed  RPHPLC method is  accurate, precise, sensitive, specific, robust and reproducible for the simultaneous analysis of Sulfadiazine and Pyrimethamine with less tailing factor and is also economical. Zodiac sil C18 column (4.6×150mm)5µ, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol: phosphate buffer(KH2PO4and K2HPO4) phosphate pH 3 (pH was adjusted with orthophosphoricacid),detection wavelength was 240nm.

 

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Published

2022-08-24