Development and validation of RP-HPLC method for simultaneous estimation of allopurinol and alphalipoicacid in bulk and tablet dosage form

Abstract

A  new  simple  ,Rapid  selective  ,  precise  and  accurate  gradient  reversed  phase  high  performance  liquid chromatographic method (RP -HPLC )method has been developed And validated for simultaneous estimation of allopurinol and alphalipoic acid in bulk and tablet dosage form. Chromatographic analysis was performed on a c-18 column (250×4.6×5 µ)at ambient temperature .the column used was as BDS in Isocratic mode, with mobile phase containing tetrabutylammoniumhydroxide buffer and acetonitrile(70:30v/v) Adjusted to Ph 6.6 with dilute orthophosphoric acid solution . The flow rate was 0.8 Ml/min and effluents were monitored at 230nm. The retention times of allopurionol and alpha lipoicacid were found to be 2.33 min and 6.32 min, respectively. The method was validated as per ICH guidelines. The recoveries of allopurinol and alpha lipoic acid were found to be 98.53 to 100.03 and 98.5 to 99.9% respectively. The proposed method was found to be accurate reproducible and consistent. It was successfully applied for the analysis of these drugs in marketed formulations and could be effectively  used  for  the  routine  analysis  of  formulations  containing  any  one  of  the  above  drugs  or  a combination, without any alteration in the chromatographic conditions.