A LC-MS compatible RP-HPLC method for the determination of ticagrelor in bulk

Abstract

A simple, rapid, accurate and precise LC -MS Compatible RP-HPLC method was developed and validated for the determination of Ticagrelor in bulk. Separation of the drug was achieved on Unisol C18 column (100 mm x4.6  mm,  5  μ)  as  stationary phase  with  mobile  p hase consisting of  ammonium acetate  buffer  pH  4.5  and acetonitrile in the ratio of 40: 60 V/V. The method showed a good linear response in the concentration range of 10-50 μg/mL with correlation coefficient of 0.99. The flow rate was maintained at 1.0 mL/m in and effluents were  monitored  at  250  nm.  The  retention time  was  3.88  min.  The  method  was  statistically validated  for accuracy, precision, linearity, ruggedness, robustness, solution stability, selectivity. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the determination of Ticagrelor in pharmaceutical dosage forms.