Development and validation of uv spectroscopy method for simvastatin in pH 6.8 phosphate buffer

Abstract

The aim of present work is to develop and validate simple, sensitive and specific Spectrophotometric method for the
determination of simvastatin, a hypolipidemic drug in pure form and in pharmaceutical formulations.UVSpectrophotometric method, which is based on measurement of absorption of U.V. The maximum wavelength in solvent
system employed for determination of simvastatin was estimated at 233 nm in pH 6.8 phosphate buffer. The linearity
range was found to be 0.01- 0.08 µg/mL(R2
=0.999). The developed method was validated with respect to linearity,
accuracy (recovery), precision and specificity. The optimum conditions for analysis of the drug were established. The drug
obeyed the Beer’s law and showed good correlation. Beer’s law was obeyed in concentration range 0.01-0.08µg/ mL The
method was found to be simple, accurate, precise, economical and robust. This method has been statistically validated and
is found to be precise and accurate.