Analytical method development and validation for the estimation of paclitaxel by using UPLC method

Abstract

A simple and precise method was developed for estimating Paclitaxel. The method was found to be specific and precise. The separation was attained on Acquity CSH C18 Column (100*2.0mm &1.5mm) and linearity was achieved in the concentration rage of 25µg/ml to 125µg/ml of Paclitaxel with correlation coefficient 0.99. The percent recovery from the assay was found to be 100.15%for Paclitaxel. Limit of detection and quantitation for Paclitaxel were within the acceptable range. From the stability studies, the percentage variation was less than 10.0% which is the desired criteria. Therefore, this method can be adopted to estimate Paclitaxelin other pharmaceutical formulations.