Advanced Method Development And Validation For Analyzing Cefixime Trihydrate And Levofloxacin Hemihydrate In Tablet Formulations Using First-Order Derivatives

Abstract

The research paper is concerned with the Analyzing Cefixime Trihydrate and Levofloxacin Hemihydrate in Tablet Formulations Using First-Order Derivatives presents a novel approach to pharmaceutical analysis through derivative spectrophotometry. This method involves transforming standard absorption spectra into first-order derivative spectra, enhancing the precision and resolution of substance identification. By analyzing the rate of absorbance change with wavelength(CEFI 297.2 nm & LEVO 288.6 nm), specific spectral features can be pinpointed, facilitating accurate quantification and characterization of substances. Utilizing a UV-visible spectrophotometer with advanced software, the study focused on the analysis of Cefixime Trihydrate and Levofloxacin Hemihydrate, common pharmaceutical compounds. The validation process adhered to international guidelines, ensuring the method's reliability and accuracy (CEFI 100.4  ± 0.94 & LEVO 100.4  ± 0.82). Calibration curves exhibited linearity over a defined concentration range for both compounds, validating the method's suitability for quantitative analysis. Overall, this study contributes to the advancement of analytical techniques for pharmaceutical quality control and research, emphasizing the pivotal role of derivative spectrophotometry in modern analytical practices.