Testicular Spermatogenic Activity of Marsilea quadrifolia Linn. against Monosodium Glutamate

Abstract

Marsilea quadrifolia is an aquatic plant that contains high antioxidants level and could prevent cell damages caused by free radicals. The present study aimed to investigate the effect of Marsilea quadrifolia ethanol extract on luteinizing hormone (LH), testosterone levels, sperm quality, and testis histology of adult male rats induced by monosodium glutamate (MSG). This study randomly divided 30 adult male rats into five groups (n6). The LH and testosterone levels at this time were measured to know the differences between the MSG and control groups. After 15 days of receiving MSG, the rats were continuously treated with MSG and followed by the administration of extract until 30 days (day 30). Both hormones between the MSG and treatment groups were measured at this time. The LH and testosterone levels significantly increased (p0.05) after Marsilea quadrifolia administration at all doses. The higher dose of M. quadrifolia ethanol extract demonstrated a high decrease in MDA level in MSG-treated rat testis increase of spermatogonia, spermatocytes, spermatids, and Leydig cells number and increase of seminiferous tubular diameter and germinal epithelium thickness. The ethanol extract of Marsilea quadrifolia can improve the levels of LH, testosterone, sperm quality, number of testis morphometric, spermatogenic, and Leydig cells in MSG-treated male rats.