https://ijpar.com/ijpar/issue/feedInternational Journal of Pharmacy and Analytical Research2026-01-06T14:44:38+00:00Prof. Dr. N.Sriram, Open Journal Systemsijparhttps://ijpar.com/ijpar/article/view/1039IMMUNOASSAYTECHNIQUES: PRINCIPLES, CLASSIFICATION, ADVANCEMENTS, AND APPLICATIONS—A COMPREHENSIVE REVIEW2026-01-06T14:44:38+00:00Venkatesh. Skodiarsan15@gmail.comL.V.Vigneshwarankodiarsan15@gmail.comMohamed Ishaq Mushrif M.N.Lkodiarsan15@gmail.comJothi Sree.Skodiarsan15@gmail.comAbinaya.Mkodiarsan15@gmail.comVinotha.Skodiarsan15@gmail.com<p>Immunoassay techniques are powerful analytical tools that exploit the high specificity of antigen–antibody interactions for qualitative and quantitative analysis of biological molecules. These techniques play a crucial role in clinical diagnostics, pharmaceutical analysis, biomedical research, food safety, and environmental monitoring. Over the years, immunoassays have evolved from classical radioimmunoassays to highly sensitive chemiluminescent, fluorescence-based, biosensor-coupled, and point-of-care systems. This review provides a comprehensive discussion on the principles, classification, working mechanisms, advantages, limitations, recent advancements, and applications of various immunoassay techniques, supported by updated scientific references.</p>2026-01-06T00:00:00+00:00Copyright (c) 2026