IJPAR JOURNAL https://ijpar.com/ijpar ijpar Prof.Dr.N.Sriram en-US IJPAR JOURNAL 2320-2831 A Comprehensive Review On Analytical Methods For The Quantification Of Favipiravir In The Drug Product, Drug Substance And In Biological Samples https://ijpar.com/ijpar/article/view/740 <p>Advanced Pharmaceutical analysis plays an important role in the quantitative estimation of drugs in the drug formulations and in biological samples. The Present review highlights the different methods used for the estimation of Favipiravir. Favipiravir is one of the drug used in the management of COVID-19 infection. Favipiravir acts by selectively inhibiting the RNA dependent RNA polymerase, an enzyme required for RNA viral replication inside human cells. It shows a broad spectrum of activity against different RNA viruses including influenza virus. The current review article provides the data for the quantification of favipiravir by using UV, HPLC and LCMS/MS methods. In this review the estimation of favipiravir in the drug substance and product can be done by using HPLC methods and in the plasma sample the drug quantification can be done by using LC MS/MS methods. This data can be used for the verification of listed methods briefly for the development of new methods for the research and development</p> V. Sravan Kumar B. Gnana Sri Siva Naga Lakshmi P. Parimala B. Moushmi U. Lavanya T. Sai Kiran P. Srinivas Babu Copyright (c) 2024 2024-01-03 2024-01-03 13 1 1 11 Development And Validation For The Simultaneous Estimation Of Fluticasone And Vilanterol In Bulk Form And In Its Pharmaceutical Dosage Form By Using RP-HPLC Method https://ijpar.com/ijpar/article/view/741 <p>A new, simple, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Fluticasone&nbsp; and Vilanterol in its pure form as well as in combined marketed formulation. Chromatography was carried out on a Phenomenex Luna C18 (4.6mm×250mm) 5µm particle size column using a mixture of Methanol: Phosphate Buffer (pH-4.2) (37:63% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 275nm. The retention time of the Fluticasone&nbsp; and Vilanterol was found to be was 2.133, 3.692 ± 0.02min respectively. The method was validated according to ICH guidelines for linearity, sensitivity, accuracy, precision, specificity and robustness. The method produce linear responses in the concentration range of 20-60mg/ml of Fluticasone and 10-30mg/ml of Vilanterol. The inter-day and intra-day precisions were found to be within limits. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations</p> N. Subhashini K.V. Nanda Kumar M. Kishore Babu Copyright (c) 2024 2024-01-03 2024-01-03 13 1 12 22 Physicochemical Evaluation and Pharmacognostic Study of Panibel (Ampelocissus latifolia) (Roxb.) -Stem https://ijpar.com/ijpar/article/view/744 <p><em>Ampelocissus latifolia</em>, belonging to the Vitaceae family, is a climber plant, mostly found in the Sub-Himalayan region of India, ranging from the Sutlej eastward to Kumaon up to 4000 feet, as well as in Aasam, Konkan, Western Ghats from Bombay to Nilgiris and Anamallis Deccan. Various parts of this plant is using to treatment of the several human diseases like leaf and stem bark is useful for wound healing<strong>, </strong>whole plant is used Kustha (leprosy) and Sotha (swelling).The stem bark is used in stomach pain and bone fracture. The roots are used in skin diseases, wound healing, rheumatic affections, fractures, diuretic, gonorrhoea, syphillis, eye diseases, menstrual troubles and also as a tonic. Ethno-medicinal and therapeutic uses of <em>Ampelocissus latifolia</em> various parts study was undertaken. In present investigation various tools and techniques included like that pharmacognostic studies, physicochemical tests, preliminary phytochemical analysis and development of HPTLC fingerprints profile.&nbsp; The established parameters can be used as standards for further study and also preparation of a monograph of <em>Ampelocissus latifolia</em> plant stem.</p> Asmita Soni Rashmi Singh Manoj Tripathi Copyright (c) 2024 2024-02-07 2024-02-07 13 1 23 29 Validated Rp-Hplc Method For Simultaneous Estimation Of Torsemide And Spironolactone In Bulk And Tablet Dosage Form. https://ijpar.com/ijpar/article/view/745 <p><strong>Background:</strong> A simple, accurate and precise HPLC method for simultaneous determination of Spironolactone and Torsemide in pure and tablet dosage form has been developed. Aim: To develop and validate analytical method for simultaneous estimation of Spironolactone and Torsemide in pharmaceutical formulation by RP-HPLC.</p> <p><strong>Materials and Methods:</strong> HPLC of Waters (Model: Alliance 2695) with Phenomenex Luna C18 (4.6 mm I.D. × 250 mm, 5 µm) column was used for chromatographic separation. It contains waters injector and PDA Detector (Deuterium). Mobile phase consists of Methanol:Water (65:35% v/v) and flow rate adjusted was 1ml/min. Wavelength selected for detection was 220nm and injection volume was 10 µl. <strong>Results and discussion:</strong> By using the developed method, retention time of Spironolactone and Torsemide was found to be 3.2min and 5.4min respectively. The method has been validated for&nbsp; linearity,&nbsp; accuracy&nbsp; and&nbsp; precision.&nbsp; Linearity of Spironolactone and Torsemide were in the range of 75–375μg/ml and 15–75μg/ml respectively. The percentage recoveries obtained for Spironolactone and Torsemide were found to be in range of 99.3 – 99.6%. LOD and LOQ were found to be 12.5µg/ml and 38.1µg/ml for Spironolactone 3.7and 11.4µg/ml for Torsemide. <strong>Conclusion: </strong>The developed HPLC method offers several advantages&nbsp; such&nbsp; as&nbsp; rapidity, usage of simple mobile phase and easy sample preparation steps. Further, improved sensitivity makes it specific and reliable for its intended use. Hence, this method can be applied for the analysis of pure drug and pharmaceutical dosage forms.&nbsp; From the present study it can be concluded that the proposed method is simple, sensitive,&nbsp;&nbsp;&nbsp; precise,&nbsp;&nbsp;&nbsp; specific,&nbsp;&nbsp;&nbsp; accurate&nbsp;&nbsp;&nbsp; and&nbsp;&nbsp;&nbsp; reproducible. Results&nbsp; of validation parameters&nbsp; demonstrated&nbsp; that&nbsp; the&nbsp; analytical&nbsp; procedure&nbsp; is&nbsp; suitable&nbsp; for&nbsp; its&nbsp; intended purpose and meets the criteria defined in ICH Q2R1.</p> Megharaj Lavanya D. Venkata Ramana K. Sai Kiran Udaya Bhanu Sri Koppu Copyright (c) 2024 2024-02-13 2024-02-13 13 1 30 40 Formulation and in vitro characterisation of milnacipran hydrochloride orodispersible tablets https://ijpar.com/ijpar/article/view/746 <p>Present study is aimed&nbsp; at the development of oral dispersible tablets of Milnacipran Hydrochloride using natural superdisintegrants. Indion 414, Polyplasdone XL 10, Primogel for the preparation of oraldispersible tablets by direct compression method. The blends were evaluated for the pre-compression parameters and all the formulations were found to possess good flow properties. Tablets were compressed by direct compression technique, evaluated for weight variation, hardness, thickness, friability, water absorption, disintegration time, dispersion time drug content and dissolution studies. The drug release profiles of the three superdisintegrants were compared. The optimized formulation F2 was showed good&nbsp; results disintegrated in 3.15 min with&nbsp; 98.89 % drug release.</p> Muthyala Amarender Goud D. Venkata Ramana K. Sai Kiran J.Pravalika Copyright (c) 2024 2024-02-13 2024-02-13 13 1 41 48 Quantitative Estimation Of Sofosbuvir And Velpatasvir In Tablet Dosage Forms By Rp-Hplc Method https://ijpar.com/ijpar/article/view/748 <p>A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Sofosbuvir and Velpatasvir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: TEA pH 4.2 (40:60) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 272 nm. The retention time of the Sofosbuvir and Velpatasvir was 2.781, 4.048 ±0.02min respectively. The method produce linear responses in the concentration range of 7.5-37.5µg/ml of Sofosbuvir and 5-25µg/ml of Velpatasvir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.</p> Kotari Abhishek D. Venkata Ramana K. Sai Kiran Udaya Bhanu Sri Koppu Copyright (c) 2024 2024-02-13 2024-02-13 13 1 49 57 Formulation And Evaluation Of Floating Microspheres Of Repaglinide By Ionic Gelation Method https://ijpar.com/ijpar/article/view/749 <p>The&nbsp; objective&nbsp; of&nbsp; the&nbsp; present&nbsp; study&nbsp; was&nbsp; to&nbsp; Prepare&nbsp; the&nbsp; alginate &nbsp;microspheres of Repaglinide (model drug) using calcium chloride as a crosslinking agent by inotropic gelation method. Microspheres were prepared by using 2%, 2.2% sodium alginate concentrations. Polymers (HPMC, Ethyl cellulose, Carbopol 934P) were used in combination concentration to prepare Microspheres. Microspheres were evaluated for micromeritic properties like angle of repose, bulk density, tapped density, Carr’s index, Hausner’s ratio and for drug content. The <em>in vitro</em> drug release study was done for microspheres All formulations. The mean particle size,&nbsp; In vitro Buoyancy, Encapsulation efficiency %, Percentage yield (%) were within limits. Among all formulations of floating microspheres F7 was considered as optimised for floating microspheres. From the release kinetics data, it was evident that floating optimised formulation follows Peppas release kinetics.</p> Sirsinigandla Bindu Sri D. Venkata Ramana K. Sai Kiran J.Pravalika Copyright (c) 2024 2024-02-22 2024-02-22 13 1 58 68