International Journal of Pharmacy and Analytical Research https://ijpar.com/ijpar ijpar Futuristic Publications en-US International Journal of Pharmacy and Analytical Research 2320-2831 IMMUNOASSAYTECHNIQUES: PRINCIPLES, CLASSIFICATION, ADVANCEMENTS, AND APPLICATIONS—A COMPREHENSIVE REVIEW https://ijpar.com/ijpar/article/view/1039 <p>Immunoassay techniques are powerful analytical tools that exploit the high specificity of antigen–antibody interactions for qualitative and quantitative analysis of biological molecules. These techniques play a crucial role in clinical diagnostics, pharmaceutical analysis, biomedical research, food safety, and environmental monitoring. Over the years, immunoassays have evolved from classical radioimmunoassays to highly sensitive chemiluminescent, fluorescence-based, biosensor-coupled, and point-of-care systems. This review provides a comprehensive discussion on the principles, classification, working mechanisms, advantages, limitations, recent advancements, and applications of various immunoassay techniques, supported by updated scientific references.</p> Venkatesh. S L.V.Vigneshwaran Mohamed Ishaq Mushrif M.N.L Jothi Sree.S Abinaya.M Vinotha.S Copyright (c) 2026 2026-01-06 2026-01-06 15 1 1 8