IJPAR JOURNAL https://ijpar.com/ijpar ijpar Prof.Dr.N.Sriram en-US IJPAR JOURNAL 2320-2831 The Analytical Method for the Simultaneous Estimation of Kanamycin and Cephalexin will be Developed by RP-HPLC Method by Optimizing the Chromatographic Conditions. https://ijpar.com/ijpar/article/view/679 <p>A new simple, selective, rapid, precise and accurate reverse phase HPLC method has been developed for simultaneous estimation of granisetron and dexamethasone. The method was developed using CPS Hypersil CN column (250×4.6 mm I.D.) with a mobile phase consisting of acetonitrile:buffer (100 mM Triethylamine adjusted to pH 3.0 with o-phosphoric acid) in ratio of 25:75 at a flow rate of 2 ml/min. Detection was carried out at 242 nm. The developed method was evaluated for various system suitability parameters and validated for linearity, accuracy, precision, LOD, LOQ as per ICH guidelines. It was also evaluated for bench top stability and freeze/thaw stability. The proposed method can be used for the estimation of these drugs in their combined dosage forms.</p> D.Curie A.Naveen Kumar D.Prsanth Kumar G.Sunny SK.Jabeena P.Ramesh Babu Copyright (c) 2023 2023-04-07 2023-04-07 12 2 131 138 Ffect Of Pancha Tulsi Against Phomphosis Azadirachtae - The Causative Agent Of Die-Back Disease https://ijpar.com/ijpar/article/view/680 <p>Neem (<em>Azadirachta indica</em>) commonly known as ‘Indian lilac’ or ‘Margosa’, is a native tree to India. Neem finds very wide application and both wood as well as non-wood products are utilized in many ways. Neem products have antibacterial, antifungal, insecticidal and other versatile biological activities<sup>1</sup>. However, neem is not free from microbial diseases though having biological activity against various microorganisms. Many bacteria and fungi are known to infect neem<sup>1</sup>. A new fungus Phomopsis azadirachtae was reported on neem causing dieback. The fungus infects the neem trees of all age and size. Twigs blight and fruit rot of <em>Azadirachta indica</em> (Neem) infected with dieback disease<sup>2</sup>, collected from different regions of Medchal, Malkajgiri district, India were analysed to determine the pathogens. The aim of this study was to evaluate the antifungal activity of Pancha tulasi essential oil on the growth of Phomopsis azadirachtae isolated from die-back infected neem twigs and fruit rot. The fungus affects leaves, twigs and inflorescence, irrespective of age, size and height of the tree. Apart from fruit rot, it causes twigs blight in neem. Study reveals the control the ’Die-back’ disease is to mix one gram of ‘Bavistin’ powder in seven/eight litres of water. This could be sprayed on the neem trees after the rainy season. After treating with chemical agents such as Thiophanate (ROKO), Profenofos (Profex) is an organophosphate insecticide, we started spraying Pancha tulasi (aromatic oils)- 5 ml in 10 litres of water for the 7 days to sustain the recovery. The disease results in almost 100% loss of fruit production.</p> Naga Kishore R Bharat Bhusan Mohapatra Abhinayani G Shalini V Copyright (c) 2023 2023-04-19 2023-04-19 12 2 139 141 Method Development and Validation For The Simultaneous Estimation of Esomeprazole and Levosulpiride By Using RPHPLC In Its Bulk And Pharmaceutical Dosage Form https://ijpar.com/ijpar/article/view/682 <p>Esomeprazole is a compound that inhibits gastric acid secretion and is indicated in the treatment of gastroesophageal reflux disease (GERD), the healing oferosive esophagitis, and H. pylori eradication to reduce the risk of duodenal ulcerrecurrence. Esomeprazole and Levosulpiride selective dopamine D2antagonist with antipsychotic and antidepressant activity.Asimple,Accurate, precise method was developed for the simultaneous estimation of the Esomeprazole and Levosulpiride in Tablet dosage form. Retentiontime of Esomeprazole and Levosulpiride were found to be 2.2min and 4.0min. % RSD of the Esomeprazole and Levosulpiride were and found to be 0.97 and 0.50 respectively. % Recover was Obtained as 100.08% and 101.16% for Esomeprazole and Levosulpiride respectively. LOD, LOQ values were obtained from regression equations of Esomeprazole and Levosulpiride were 0.10ppm, 0.34ppm and 0.29ppm, 1.04ppm respectively. Regression equation of Esomeprazole is y = 10568x + 307.3 and of Levosulpiride is y = 11649.x+ 1207. Retention times are decreased and thatrun time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.</p> Gosavi Sureshpuri Anandpuri Rakesh Patel Jeevan Patel Jeevan Patel Mohit Chaturvedi Gourav Jain Copyright (c) 2023 2023-04-24 2023-04-24 12 2 142 150 DNA programming in drug discovery and drug development https://ijpar.com/ijpar/article/view/684 <p>Important traits that biological cells have that make them appealing for many applications are energy efficiency, self-reproduction, and small size. Examples include the incorporation of intelligence into smart materials, sensing, and nanoscale manufacturing. Simple "genetic circuits" that are encoded in DNA and carry out their functions in the cellular environment have been developed over the past 15 years.&nbsp; A gene circuit uses biological interactions to provide computation, similar to an electronic circuit. Simulators that replicate cell colonies running microbial programmes have been developed at the protein and language levels.</p> Ravi kumar maddali Naga kishore R Copyright (c) 2023 2023-04-29 2023-04-29 12 2 151 153 Method Development and Validation For The Quantification Of Pantoprazole By RP-HPLC And Spectrophotometry In Pharmaceutical Oral Dosage Form https://ijpar.com/ijpar/article/view/686 <p>Simple, Precise, rapid and accurate methods were developed for the estimation of Pantoprazole in bulk and in dosage form. The methods are UV Spectroscopic method, Visible Spectroscopic method &amp; RP-HPLC method. The λmax of of PNP was found to be 290nm in methanol. The bulk drug PNP obeyed Beer’s law at 5-30µg / ml. The correlation coefficient was found to be 1 for both the drugs. The dosage form of the drugs was quantified by the following three methods. Method A involves standard absorbance method. The RSD proves there producibility of the method and thus the precision of the developed method. Method B involves determination of AUC for both standard and sample spectra obtained in Method A between two selected wavelength. The correlation coefficient was found to be 0.9990 for PNP. The recovery percentage was found to be 98 to102% which proves no interference by the sample matrix. Method C involves the derivatisation of the zero order spectra to second order spectra. &nbsp;The recovery spectra were also derivatized and used. All the methods have shown good linearity, precision and accuracy. The low % RSD values in recovery studies for all the above methods indicate that there is no interference due to excipients used in the formulations. Hence it is concluded that the developed UV – Visible and RP-HPLC methods were found to be simple, precise, accurate and rapid methods for the analysis of Pantoprazole in its pure form and in its pharmaceutical dosage formulation. Thus, all the above adopted methods can be effectively used for the routine analysis of Pantoprazole in pharmaceutical dosage form.</p> Patil Akshay Kishore Rakesh Patel Mohit Chaturvedi Jeevan Patel Gaurav Jain Copyright (c) 2023 2023-05-03 2023-05-03 12 2 154 166 Formulation of transdermal approach for the treatment of angina using nifedipine as a model drug https://ijpar.com/ijpar/article/view/687 <p>The purpose of this research was to develop a matrix-type transdermal therapeutic system containing drug Nifedipine with different ratios of polymeric systems by the Solvent evaporation technique by using Dibutyl phthalate to the polymer weight, incorporated as plasticizer. Glycerol was used to enhance the transdermal permeation of Nifedipine.The physicochemical compatibility of the drug and the polymers studied by infrared spectroscopy suggested absence of any incompatibility. Formulated transdermal patches were physically evaluated with regard to thickness, weight variation, drug content, flatness, tensile strength and folding endurance. <em>In-vitro</em> drug studies of formulations were performed by using Franz diffusion cells. The results followed the release profile of Nifedipine followed mixed peppas release kinetics. However, the release profile of the optimized formulation F6 (98.87% at 12hr) indicated that the permeation of the drug from the patches was governed by a diffusion mechanism.</p> PriyoDutta Mithun Bhowmick Sandip Karmakar Pratibha Bhowmick Copyright (c) 2023 2023-05-13 2023-05-13 12 2 167 176 Formulation development and characterization of voriconazole nanoparticles https://ijpar.com/ijpar/article/view/688 <p>Nanoparticles represent a promising drug delivery system of controlled and targeted drug release. They are specially designed to release the drug in the vicinity of target tissue. The aim of this study was to prepare and evaluate Carbopol p934 nanoparticles containing Voriconazole in different drug to polymer ratio. SEM indicated that nanoparticles have a discrete spherical structure. FT-IR studies indicated that there was no chemical interaction between drug and polymer and stability of drug. The <em>in vitro </em>release behavior from all the drug loaded batches was found to be first order release and provided sustained release over a period of 12 h. The developed formulation overcome and alleviates the drawbacks and limitations of Voriconazole sustained release formulations and could possibility be advantageous in terms of increased bioavailability of Voriconazole</p> Mobarak Alam Mallick Mithun Bhowmick Tulshi Chakraborty Soumen Dey Pratibha Bhowmick Copyright (c) 2023 2023-05-13 2023-05-13 12 2 177 186 Formulation and characterization of ethylcellulose based transdermal patches of atenolol for improved invitro skin permeation https://ijpar.com/ijpar/article/view/689 <p>The skin can be used as the site for drug administration for continuous transdermal drug infusion into the systemic circulation. For the continuous diffusion penetration of the drugs through the intact skin surface membrane-moderated systems, matrix dispersion type systems, adhesive diffusion controlled systems and micro reservoir systems have been developed. Various penetration enhancers are used for the drug diffusion through skin. In matrix dispersion type systems, the drug is dispersed in the solvent along with the polymers and solvent allowed to evaporate forming a homogeneous drug-polymer matrix. Matrix type systems were developed in the present study. In the present work, an attempt has been made to develop a matrix-type transdermal therapeutic system comprising of Atenolol with different concentration of various polymers alone using solvent evaporation technique. The physicochemical compatibility of the drug and the polymers was studied by infrared spectroscopy. The results obtained showed no physical-chemical incompatibility between the drug and the polymers. F1formulation has been selected as the best formulation among all the other formulations. The <em>in vitro</em> drug diffusion studies from the formulation were found to be sustained release. All the evaluation parameters obtained from the best formulation were found to be satisfactory.The data obtained from the <em>in vitro</em> release studies were fitted to various kinetic models like zero order, first order, Higuchi model and peppas model. From the kinetic data it was found that drug release follows peppasmodel release by diffusion technique from the polymer.</p> Kritika Modak Mithun Bhowmick Soumik Laha Pratibha Bhowmick Copyright (c) 2023 2023-05-13 2023-05-13 12 2 187 196 Development and validation of a new simple, sensitive and validated RP-HPLC method for the estimation of moxonidine in bulk form and marketed formulation https://ijpar.com/ijpar/article/view/690 <p>A novel, simple, accurate, precise, sensitive and specific analytical RP-HPLC method was developed and validated for the quantitative estimation of Moxonidine in bulk drugs and pharmaceutical dosage form. Chromatographic separation was achieved on an Symmetry ODS C18 (4.6×250mm, 5µm) analytical column using mobile phase composition of methanol and Phosphate Buffer in ratio of (35: 65 v/v) that was set at a flow rate of 1.0μl/min with detection of 235 nm. The retention time of Moxonidine was found to be 3.006min. The drug was analyzed by following the guidelines of International conference on Harmonization (ICH). This drug showing linearity in the concentration range of 6-14µg/ml and the correlation coefficient showing R2 = 0.9996. The % Recoveries showing within the limits. The presentation of the method was validated according to the present ICH guidelines for accuracy, precision and robustness, Linearity, limit of quantification, limit of detection linearity.</p> Ramjoshi Sravani Gade Sammaiah Copyright (c) 2023 2023-05-29 2023-05-29 12 2 197 206 Development and validation for the quantitative determination of tivozanib in bulk form and marketed pharmaceutical dosage form by RP-HPLC https://ijpar.com/ijpar/article/view/692 <p>A simple, rapid, specific and accurate reverse phase high performance liquid chromatographic method has been developed for the validated of Tivozanib in bulk as well as in marketed pharmaceutical dosage form. This separation was performed on a Symmetry ODS (C18) RP Column, 250 mm x 4.6 mm, 5µm column with Acetonitrile, Methanol and 0.1% OPA in the ratio of 60:30:10 as mobile phase at a flow rate of 1.0 mL min−1 with UV detection at 235 nm; the constant column temperature was Ambient. The run time under these chromatographic conditions was less than 6.0 min. The retention time of Tivozanib was found to be 2.570min. The calibration plot was linear over the concentration range of 6–14 μg mL−1 with limits of detection and quantification values of 0.8 and 0.24ng mL−1 respectively. The mean % assay of marketed formulation was found to be 99.79%, and % recovery was observed in the range of 98-102%. Relative standard deviation for the precision study was found &lt;2%.The developed method is simple, precise, specific, accurate and rapid, making it suitable for estimation of Tivozanib in bulk and marketed pharmaceutical dosage form dosage form.</p> Kolkulapally Hym Gade Sammaiah Copyright (c) 2023 2023-05-30 2023-05-30 12 2 207 216