IJPAR JOURNAL

Advanced Method Development And Validation For Analyzing Cefixime Trihydrate And Levofloxacin Hemihydrate In Tablet Formulations Using First-Order Derivatives

N. Elavarasan, Geolin Justin Babu, J. Ansal Shakina, V.L. Saranya — Mon, 15 Jul 2024 00:00:00 +0000

The research paper is concerned with the Analyzing Cefixime Trihydrate and Levofloxacin Hemihydrate in Tablet Formulations Using First-Order Derivatives presents a novel approach to pharmaceutical analysis through derivative spectrophotometry. This method involves transforming standard absorption spectra into first-order derivative spectra, enhancing the precision and resolution of substance identification. By analyzing the rate of absorbance change with wavelength(CEFI 297.2 nm & LEVO 288.6 nm), specific spectral features can be pinpointed, facilitating accurate quantification and characterization of substances. Utilizing a UV-visible spectrophotometer with advanced software, the study focused on the analysis of Cefixime Trihydrate and Levofloxacin Hemihydrate, common pharmaceutical compounds. The validation process adhered to international guidelines, ensuring the method's reliability and accuracy (CEFI 100.4 ± 0.94 & LEVO 100.4 ± 0.82). Calibration curves exhibited linearity over a defined concentration range for both compounds, validating the method's suitability for quantitative analysis. Overall, this study contributes to the advancement of analytical techniques for pharmaceutical quality control and research, emphasizing the pivotal role of derivative spectrophotometry in modern analytical practices.

Therapeutic Potential of Heeraka Bhasma (Incinerated Diamond ash): A review through Classical and Contemporary literature.

Rakshitha Shetty, G. Priyadarshini, R. Koirala, K.S. Sakhitha, S. Kumar — Mon, 15 Jul 2024 00:00:00 +0000

The ancient Indian alchemical tradition of Rasashastra encompasses a diverse literature on the therapeutic application of metals, minerals, and gemstones. This tradition later integrated into Ayurveda, the indigenous medical system of India, forming a significant branch thereof. Within Rasashastra's classical texts, Heeraka (Diamond) is categorized under Ratna Varga (Precious gemstones classification), alongside other precious and semiprecious gemstones. Notably, Heeraka Bhasma, the incinerated ash of diamond, finds mention for treating internal abscesses, tumors, angina pectoris, and tuberculosis. Reviewing over 30 classical texts reveals various pharmaceutical procedures like Shodhana(Purification) and Marana(Process of preparing Bhasma), enhancing Heeraka's therapeutic potential. Common purification methods include Nirvapa(Hearing and Quenching) and Swedana(Boiling), while Gajaputa(Amount of Heat) is the commonest puta given for Bhasma(Incinerated Ash) preparation, often employing Karpasa Mula and 3-year-old Nagvalli swarasa as media. This review aims to comprehensively explore diamond's processing techniques and therapeutic attributes as described in classical literature and contemporary research.

Synthesis, Characterization and Biological evaluation of New Schiff’s Base Derivatives

M. Mohanapriya, R. Manivannan, C. Praveen Kumar, T. Ranjith, C. Udhaya — Tue, 16 Jul 2024 00:00:00 +0000

This manuscript presents the synthesis, characterization, and biological evaluation of novel Schiff’s base derivatives derived from aromatic aldehydes and amino acids. Schiff bases, known for their biological relevance and stability when linked to aryl groups, were synthesized using a condensation reaction facilitated by mildly acidic conditions to ensure optimal yields without compromising the nucleophilicity of the amines. The synthesized compounds were rigorously characterized using melting point determination, thin-layer chromatography (TLC), and infrared spectroscopy (IR). Their biological efficacy was assessed through in vitro antibacterial and antifungal assays, demonstrating considerable activity against common pathogenic strains such as Escherichia coli, Bacillus subtilis, and Candida albicans. The compounds exhibited a range of activities, with some showing comparable efficacy to standard antibiotics and antifungals. These findings suggest that Schiff’s bases could be potent candidates for drug development, offering a scaffold for further chemical enhancement and therapeutic application.

A review on analytical method development and validation of pantaprazole in bulk and tablet formulation by using Uv- spectrophotometry

A. Raja Reddy, S. UdayKiran, Sabitha Sabu, T. Rama Rao — Mon, 22 Jul 2024 00:00:00 +0000

Pantoprazole (Protonix) is an irreversible proton pump inhibitor (PPI) that reduces gastric acid secretion. in conjunction with a pair of antimicrobial medicines, most frequently amoxicillin, clarithromycin, or metronidazole. Analysis can be done by various methods. Most commonly used solvents are HCl, NaOH, Methanol, Water. Various parameters are collected including LOD, LOQ, Correlation Coefficient and linearity range, accuracy, Ruggedness.

Development and Validation of Simple and Precise UV Spectroscopy Method for Quantifying Baicalein & Berberine in Bulk and Formulated Products

Fri, 26 Jul 2024 00:00:00 +0000

A simple, precise, and cost-effective UV-visible spectrophotometric method was developed and validated for the simultaneous estimation of baicalein and berberine in bulk and formulations, in accordance with ICH Q2 (R1) guidelines. Calibration standards of baicalein and berberine were prepared, and calibration curves of concentration versus absorbance were plotted. Various analytical method validation parameters such as linearity, accuracy, precision, robustness, ruggedness, limit of detection, and limit of quantitation were calculated. The maximum wavelengths for baicalein and berberine were found to be 274 nm and 344 nm, respectively. The correlation coefficients over the concentration ranges of 0.5-8 μg/ml and 5-30 μg/ml were 0.998 and 0.999 for baicalein and berberine, respectively. The developed UV method demonstrated high precision, accuracy, and sensitivity, with % RSD less than 2. This validated UV-visible method can be efficiently used for the individual and simultaneous estimation of baicalein and berberine in bulk and formulations.

Targeting On The Regulation On Testicular Spermatogenic Activity Against Monosodium Glutamate Adverse Effects

Mon, 05 Aug 2024 00:00:00 +0000

Monosodium glutamate (MSG) is a well-known food additive that has been shown to be effective in causing male reproductive abnormalities. This common flavor enhancer is used extensively in many diets to improve flavor. There are various mechanisms through which it can cause reproductive dysfunctions. These include spermatogenic alteration, which can lead to abnormalities such as low sperm count, high sperm count, reduced live sperm and decreased sperm pH; oxidative damage, which can involve increased lipid peroxidation and decreased antioxidant enzyme activities; histological alteration, which can involve blood clots, distorted germ cells, and Sertoli cells; and gonadotropin imbalance, which can involve decreased levels of follicle-stimulating hormone, testosterone and luteinizing hormone. The different known pathways through which MSG may cause reproductive dysfunction are therefore covered in this review along with mitigation techniques for its harmful consequences

In Vitro and In Vivo Evaluation of anti-diabetic Potential of Pisonia grandis

A. Kavidha, G. Nagaraja Perumal — Mon, 05 Aug 2024 00:00:00 +0000

The general interest in plants stems from their fundamental role in the commencement of life and their ability to produce various chemicals that act on the body, aiding in the treatment of diseases. The extraction of leaves from Pisonia grandis was conducted using various solvents with the Soxhlet apparatus. All extracts showed positive results in both in vitro and in vivo pharmacological studies. Our research findings indicate that for 50% inhibition of alpha-amylase activity, the concentrations were as follows: Ethyl acetate extract at 91 µg/ml, water extract at 80 µg/ml, petroleum ether extract at 75 µg/ml, and Acarbose (positive control) at 61 µg/ml. Our final report shows that the ethanolic extract has the highest anti-diabetic activity compared to the other extracts due to the presence of more anti-diabetic bioactive components. We conclude that this work will be useful in discovering novel entities for the treatment of diabetes mellitus.

Reviewing the development and validation of analytical methods for different drugs utilizing uv spectroscopy

Banothu Bhadru, Tadikonda Rama Rao, Sushma Jakinaboina — Wed, 14 Aug 2024 00:00:00 +0000

UV-visible spectrophotometry refers to absorption spectroscopy. It provides fast and efficient analysis. It is used to quantify nucleic acid and protein content in biological samples and for quality control in drugs and food industries. It is based on the interaction between light and matter. It is used in the wavelength range of 190-900nm. Many drugs are analyzed by UV visible spectrophotometer. Fenofibrate is an oral medication of the fibrate class used to treat abnormal lipid levels. It works by increasing the natural substance that breaks down the fats in the blood. It is less commonly used when compared with statins because it treats a different type of cholesterol abnormality than statins. Ranolazine is an anti-anginal drug. It is used to treat chronic angina (cardiovascular illness). It is used concomitantly with angiotensin receptor β-blockers, and ACE inhibitors. Pantoprazole is an anti-ulcer (proton pump inhibitor). It irreversibly inhibits the gastric H⁺/K⁺-ATPase inhibitors. It is metabolized in the liver and excreted through urine.

A Prospective Study of on-Label and off-Label Drug use in Paediatrics at Tertiary Care Hospital

Perli Kranti Kumar, Naga Latha Dhulipalla, Veena Gadicherla, Varun Dasari — Fri, 16 Aug 2024 00:00:00 +0000

The study to be undertaken with an aim of investigation of incidence and pattern of off-label drug use, to identify drugs most commonly used as off-label, and to identify factors associated with off-label drug use in Indian paediatrics population at tertiary care hospital.This study was conducted in Inpatient Paediatrics Department of S.N.Medical College and H.S.K Hospital and Research Centre, Bagalkot. According to age group, use of licensed and unlicensed medication out of 1205 medications, 851 are licensed medications of which 415 (34.43%) were used for neonates, 308(25.56%) infants, 100 (8.29%) young child, 28 (2.32%) child. 16 are unlicensed medications of which 4 (0.33%) were used for neonates, 5 (0.41%) infants, 6 (0.49%) young child & 1 (0.08%) child. 338 are off-labelled medications out of this 124 (10.29%) were neonates, 173 (14.35%) were infants, 30 (2.48%) were young child, and 11 (0.91%) are child.The beneficial effects of studying off-label drugs that are being prescribed to children include important dose changes, frequency, as well as improved safety information on how to more appropriately prescribe these drugs for the paediatric population.

A New Validated UV-spectroscopic Method for Simultaneous Estimation of Tenegliptin and Metformin in Pharmaceutical Dosage Form

Sun, 18 Aug 2024 00:00:00 +0000

Simple, precise, economical, fast and reliable UV-Spectroscopic methods have been developed for the simultaneous estimation of Metformin HCl and Tenegliptin in bulk and pharmaceutical dosage form. The method which is based on measurement of absorption at maximum wavelength of 232 nm and 242 nm for Metformin HCl and Tenegliptin respectively. Linearity for detector response was observed in the concentration range of 2-10μg/ml for Metformin HCl and 2-10 μg/ml for Tenegliptin. The accuracy of the methods was assessed by recovery studies and was found to be 100.23 % and 99.36% for Metformin HCl and Tenegliptin respectively. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. The results were validated statistically as per ICH Q2 R1 guideline and were found to be satisfactory. The proposed methods were successfully applied for the determination of for Metformin HCl and Tenegliptin in commercial pharmaceutical dosage form

Isolation and Characterization of Fenugreek Seed Mucilage, A Natural Mucoadhesive Polymer.

Jooly Kurien, Rakesh Kumar Jat, Boby Johns G — Thu, 29 Aug 2024 00:00:00 +0000

This study focuses on isolating and characterising fenugreek seed mucilage, a natural mucoadhesive polymer derived from Trigonella foenum-graecum. The extraction process of mucilage, which involved using deionized water followed by precipitation with acetone, demonstrated high efficiency, yielding 31.78% mucilage from the seeds. Characterization of the mucilage confirmed the presence of carbohydrates and mucilaginous properties, with an ash value indicating high purity. The mucilage displayed favourable micromeritic properties, such as good flowability and low moisture content, which are essential for pharmaceutical applications. Its pH and swelling index suggest compatibility with gastrointestinal environments. Comparative analysis revealed that fenugreek seed mucilage has mucoadhesive strength similar to Carbopol 934 P and significantly higher than sodium alginate, making it a promising candidate for use in drug delivery systems.

Rare Case Report On Median Arcuate Ligament Syndrome – A Surgical Approach

Amrutha Gopal, Rubina Mariam Oommen — Thu, 29 Aug 2024 00:00:00 +0000

Median Arcuate Ligament Syndrome (MALS), also known as celiac artery compression syndrome or Dunbar syndrome, is a rare vascular disorder characterized by compression of the celiac artery by the median arcuate ligament. This compression can lead to a range of symptoms including postprandial abdominal pain, weight loss, nausea, and vomiting. We present a case of a 57-year-old female patient who presented with complaints of left hypochondrial and left lumbar pain. Her medical history was significant for newly diagnosed diabetes and bilateral salpingo-oophorectomy. Upon further evaluation, CT abdomen revealed narrowing of the celiac trunk due to an overlying thickened median arcuate ligament, confirming the diagnosis of MALS.

Surgical management was pursued with the laparoscopic-assisted release of the median arcuate ligament under general anesthesia. The procedure successfully decompressed the celiac trunk, alleviating the patient's symptoms. Postoperatively, the patient had an uneventful recovery and was discharged with general instructions. This case underscores the importance of recognizing MALS in patients presenting with characteristic symptoms and the efficacy of surgical intervention in providing relief and improving quality of life.

Role of Artificial Intelligence (AI) Tools and Machine Learning (ML) in Clinical Trials for FDA Approval of Pharmaceutical Drugs: A Comprehensive Review

Rahul Swamy Tirunagari — Mon, 02 Sep 2024 00:00:00 +0000

Over the past few decades, India's pharmaceutical sector has seen major technological innovations in drug development. India now holds a major place in the world for pharmaceutical research and development as a result of these developments. The purpose of this abstract is to give a general overview of some significant technological advancements in the Indian pharmaceutical sector, with a focus on drug development. The growing use of artificial intelligence (AI) and machine learning (ML) algorithms in several phases of drug research and development is one noteworthy advance. These technologies make things quicker and more precise. furthermore, the production of drug and development of drug to the unique techniques. Proteomics and genomics developments have also enabled innovative drugs development methods by enabling the production of drug customized or drug combinational to the unique profiles of each subject. India is becoming a major participant in the global pharmaceutical scene thanks to technical developments in drug discovery, which have also fuelled advances in safety, personalized medicine, and therapeutic efficacy. Yet issues like infrastructure development of drugs and Ethical and safety and AI and ML Validation and Government and Regularity authority Guidance and regulatory compliance still exist, underscoring the necessity of ongoing funding and cooperation in the pharmaceutical industry. This research work aims to study the AI and ML approaches in clinical trials for a FDA approval. The objectives are studied by conducting a interview with the participants from the industry personnel. Key findings from the research are that training and awareness are required in the industry about the AI involvement in the clinical trials. there should be a regulatory compliance maintained to achieve smooth and diligent protocol for clinical trials with AI for FDA approvals.

Exploring imeglimin efficacy; A case series from a tertiary care hospital

Anuvidya. R, Abhirama .B.R, Muhzina Rahim, Amrutha Gopal, Rubina Mariam Oomen — Tue, 03 Sep 2024 00:00:00 +0000

Imeglimin, a tetrahydro triazine containing drug, is a novel therapeutic agent for the treatment of Type2 Diabetes Mellitus. Imeglimin has been demonstrated to improve three main pathogenetic components of T2DM: increased gluconeogenesis, inadequate glucose-induced insulin production in beta cells, and peripheral insulin resistance. This case series aimed to evaluate the efficacy and safety of imeglimin in a real-world clinical setting among patients with type 2 diabetes who had inadequate glycemic control with their current treatment regimen. The cases are collected from a tertiary healthcare centre in Kerala, South India. Insights gleaned from the case series indicate that imeglimin significantly improves glycemic control, enhances insulin sensitivity, and preserves beta-cell function in patients with type 2 diabetes