New method for simultaneous determination of Rabeprazole and Itopride in pharmaceutical dosage form by UV-visible absorption spectrophotometry
Keywords:UV-visible Spectrophotometry, Rabeprazole, Itopride, Concentration ratio, Absorbance ratio
A simple, accurate and rapid spectrophotometric method for simultaneous determination of Rabeprazole (RAB) and Itopride (IPD) in binary mixture has been developed. Two calibration curves were carried out; the first was elaborated to determine Rabeprazole in the mixture at 462 nm and the second was used to determine the concentration ratio (CR) in the mixture at 419 nm. This last was plotted by determination of the absorbance ratio (AR) against the concentration ratio CR= [RAB]/ [IPD]. Finally the concentration of the RAB was deduced [RAB] = CRexp × [IPD]. The developed method has been validated and has given satisfactory results. The results of analysis formulation given as percentage of label claim were found to be 99.85% and 99.67% for RAB and IPD respectively for AR1, 99.54 for RAB and 99.74 for IPD for AR2. The suggested method could be used in the routine quality control analysis of pharmaceutical formulation.