New method for simultaneous determination of Rabeprazole and Itopride in pharmaceutical dosage form by UV-visible absorption spectrophotometry

Authors

  • Imam Pasha. S Sultan -Ul-Uloom College of Pharmacy, Mount Pleasant, Road No.3, Banjara Hills, Hyderabad-500 034, Telangana, India.
  • Amtus Salam Fatima Sultan -Ul-Uloom College of Pharmacy, Mount Pleasant, Road No.3, Banjara Hills, Hyderabad-500 034, Telangana, India.
  • Seema Mehveen Sultan -Ul-Uloom College of Pharmacy, Mount Pleasant, Road No.3, Banjara Hills, Hyderabad-500 034, Telangana, India.
  • Saba Ahmed Sultan -Ul-Uloom College of Pharmacy, Mount Pleasant, Road No.3, Banjara Hills, Hyderabad-500 034, Telangana, India.
  • Maleeha Asadullah Sultan -Ul-Uloom College of Pharmacy, Mount Pleasant, Road No.3, Banjara Hills, Hyderabad-500 034, Telangana, India.
  • Anupama Koneru Sultan -Ul-Uloom College of Pharmacy, Mount Pleasant, Road No.3, Banjara Hills, Hyderabad-500 034, Telangana, India.

Keywords:

UV-visible Spectrophotometry, Rabeprazole, Itopride, Concentration ratio, Absorbance ratio

Abstract

A simple, accurate and rapid spectrophotometric method for simultaneous determination of Rabeprazole (RAB) and Itopride (IPD) in binary mixture has been developed. Two calibration curves were carried out; the first was elaborated to determine Rabeprazole   in the mixture at 462 nm and the second was used to determine the concentration ratio (CR) in the mixture at 419 nm. This last was plotted by determination of the absorbance ratio (AR) against the concentration ratio CR= [RAB]/ [IPD]. Finally the concentration of the RAB was deduced [RAB] = CRexp × [IPD]. The developed method has been validated and has given satisfactory results. The results of analysis formulation given as percentage of label claim were found to be 99.85% and 99.67% for RAB and IPD respectively for AR1, 99.54 for RAB and 99.74 for IPD for AR2. The suggested method could be used in the routine quality control analysis of pharmaceutical formulation.

Downloads

Published

2022-09-01