Stability indicating RP-HPLC method development and validation for simultaneously estimation of ivabradine and metaprolol in bulk and pharmacetical dosage form

Authors

  • T. Ram Mohan Reddy Department of Pharmaceutical Aanalysis, CMR College of pharmacy, Kandlakoya(V), medchal(D), Telangana(S).
  • Eldurthi Manoj Chandu Department of Pharmaceutical Aanalysis, CMR College of pharmacy, Kandlakoya(V), medchal(D), Telangana(S).

DOI:

https://doi.org/10.61096/ijpar.v8.iss3.2019.241-251

Keywords:

Ivabradine, Metoprolol, RP-HPLC

Abstract

A Stable, selective, easy and accurate method was developed for the simultaneous estimation of the Ivabradine and Metoprolol in tablet dosage form. Chromatogram was run through Discovery C8 250mm x 4.6 mm, 5mm. phosphate buffer and  Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1.0 ml/min. 260nm selected as optimum wavelength. 2.517min and 3.097min are retention time of Ivabradine and Metoprolol. 99.65% and 99.39 % recovery values for Ivabradine and Metoprolol respectively. 0.31, 0.95ppm and 0.68, 2.06 are LOD and LOQ values for Ivabradine and Metoprolol.  Regression equation of Ivabradine is y = 17301x + 1713 and y = 17366x + 3213 of Metoprolol. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.  

Downloads

Published

2022-09-12

How to Cite

T. Ram Mohan Reddy, & Eldurthi Manoj Chandu. (2022). Stability indicating RP-HPLC method development and validation for simultaneously estimation of ivabradine and metaprolol in bulk and pharmacetical dosage form. IJPAR JOURNAL, 8(3), 241–251. https://doi.org/10.61096/ijpar.v8.iss3.2019.241-251

Issue

Vol. 8 No. 3 (2019): 2019 Volume - 8 Issue - 3

Section

Articles