Chemometric assisted RP-HPLC quantitative estimation and validation of hydrochlorothiazide and triamterene in tablet dosage form

Abstract

A new chemometric assisted by high-performance liquid chromatography (HPLC) with photodiode array (PDA) detection was implemented for the simultaneous determination of Hydrochlorothiazide and Triamterene in tablet dosage form. Two chemometric calibration techniques, principle component analysis (PCA) and partial least square analysis (PLS) were applied to the peak area at 276 nm of PDA detector responses. The method was carried out on a Luna C18, 250mm x 4.6mm, 5µm, column with a mobile phase consisting of Acetonitrile and Buffer in the ratio of (25  :75v/v) and  flow rate  of 1.0  ml/  min. The detection was carried out  at  272  nm. The retention time  for Hydrochlorothiazide and Triamterene were found to be 4.1and 6.2min respectively. The method was validated according to the ICH guidelines for specificity, LOD, LOQ, precision, accuracy, linearity and robustness. The method showed good reproducibility and recovery with %RSD less than 2. So the proposed method was found to be simple, specific, precise, accurate and  linear. The ‘UNSCRAMBLER -X(camo)’ software was used  for  the  numerical calculations.  All  of  the  two-chemometric analysis  methods  in  this  study can  be  satisfactorily applied  for  the quantitative analysis of Hydrochlorothiazide and Triamterene in pharmaceutical tablet dosage form.