Analytical Method Development and Validation for Simultaneous Estimation of Lercandipine and Atenolol Tablet Dosage Form by RP-HPLC

Abstract

estimation of Lercanidipine and Atenolol stimultaneously in combined dosage form. A kromosil C-8 column
having dimensions of 4.6µmx250mm and particle size of 5µm in isocratic mode , with mobile phase containing
a mixture of Di- Potassium Hydrogen Phosphate and Acetonitrile in ratio of (70:30v/v) (pH adjusted to pH
6.5±0.5 using phosphoric acid was used . Detection was done at 215 nm using PDA detector. The mobile phase
was pumped at a flow rate of 1.0µl/min and injection volume is10µl. The selected chromatographic conditions
were founds to effectively separate Lercandipine (Rt 3.8min)(2) and Atenolol (Rt: 6.1 min) having resolution of
7.8. The method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and
limit of quantitation.