New RP-HPLC method development and validation for trioxsalen in tablet dosage form

Authors

  • Senthil Kumar KK Sun Rise University, Alwar-301030, Rajasthan, India.
  • Perumal P Sun Rise University, Alwar-301030, Rajasthan, India.

DOI:

https://doi.org/10.61096/ijpar.v10.iss1.2021.38-46

Keywords:

UV -Ultraviolet visible, µg -Micro gram, ml- Milliliter, nm- Nano meter, RP-HPLC-Reverse phase chromatography

Abstract

A new simple, accurate, rapid and precise Gradient High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Trioxsalen C18 Column (4.6 x 150 mm and 3.5 µm) and flow rate of 1.2 ml/min with a load of

20µl. phosphate buffer (PH-3.9) was used as mobile phase A Methanol was used as Mobile Phase B and Diluent as Water: acetonitrile in the composition of 50:50. The Detection was carried out at 248nm.This newly developed method was successfully utilized for the Quantitative estimation of Trioxsalen in pharmaceutical dosage forms. This method was validated as per ICH guidelines.

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Published

2022-08-24

How to Cite

Senthil Kumar KK, & Perumal P. (2022). New RP-HPLC method development and validation for trioxsalen in tablet dosage form. IJPAR JOURNAL, 10(1), 38–46. https://doi.org/10.61096/ijpar.v10.iss1.2021.38-46

Issue

Vol. 10 No. 1 (2021): 2021 Volume - 10 Issue - 1

Section

Articles