Effect of different sampling locations on the antibacterial property of Centella asiatica leaves

Abstract

An experiment was carried out to determine the effect of antibacterial activity of Centella asiatica herb
collected from different sampling location. The effect can be determined by analysing the bacterial response
towards plant extracts. The tested bacterial strains were Staphylococcus aureus, Bacillus subtilis, Escherichia
coli and Salmonella typhi. Zone of inhibition produced by different extracts against the selected strains was
measured and compared with standard antibiotic penicillin (10μg) and gentamicin (10μg). The result
demonstrated that the Centella asiatica from urban area have higher antimicrobial activities (average of 1.629
cm zone of inhibition) than sub-urban (average of 1.305 cm zone of inhibition). All the extracts showed better
results against the Escherichia coli strains comparing with penicillin (10μg) but not for gentamicin (10μg) test
on the other bacteria strains. The results obtained in the present study suggest that the different extracts of
Centella asiatica revealed a significant scope to develop a novel broad spectrum of antibacterial herbal
formulations.