METHOD DEVELOPMENT AND VALIDATION ON ETOMIDATE INJECTION BY RP-HPLC

Abstract

A new simple, accurate, rapid and precise isocratic High performance  liquid chromatographic  (HPLC) method was developed and validated for the determination  of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile  and Phosphate buffer was used as mobile phase in the composition  of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate  was 40-240 µg/ml respectively.  Retention  Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully  utilized for the Quantitative  estimation of Etomidate in injectables.  This method was validated for accuracy, precision, linearity and Robustness  as per ICH guidelines.