A review on analytical method development and validation of Nimodipine using UV Spectroscopy

Abstract

A simple, specific, precise and efficient UV Spectrophotometric method has been developed and validated for the quantification of Nimodipine in pharmaceutical products. The absorption peak for Nimodipine was found at 239.0 nm, exhibiting a strong linear relationship with a correlation coefficient of 0.9996. The validation process encompassed studies on precision and accuracy, and optimal analytical conditions were established. The maximum wavelength (λ max) for Nimodipine was identified as 238.5 nm. The method complied with Beer’s law over a concentration range of 5-30 mcg/mL, represented by the linear equation y = 0.033x + 0.020 and a correlation coefficient of 0.9981. Furthermore, parameters such as slope, intercept, correlation coefficient, detection limits, and quantification limits were determined. The analytical results were statistically validated and corroborated through a recovery study. This method is deemed appropriate for the routine analysis of Nimodipine in tablet formulations.