The RP-UPLC method for simultaneous quantification of ivabradine and metoprolol

Abstract

In order to develop a newer or improved analytical method, the analyst has to set some goals. The method should be precise to the drug under study. It is necessary to determine the analyte at trace levels accurately. The UPLC techniques have now become extremely reliable and indispensable. Ivabradine, lowers the pacemaker firing rate, consequently lowering heart rate and reducing myocardial oxygen demand. Metoprolol is a beta-1-adrenergic receptor inhibitor specific to cardiac cells with negligible effect on beta-2 receptors. Run time was selected to be 3 min because the analysis gave peaks around 1.197 and 1.628 ±0.02 min of ivabradine and metoprolol. The analytical method was found to be linear over the range 1.25-7.5 µg/mL for ivabradine and 6.25-37.5 µg/mL for metoprolol of the target concentration.