Simultaneous determination and validation of third generation antiviral drugs by RP-HPLC method

Abstract

A simple reverse phase high pressure liquid chromatography (RP-HPLC) method has been developed and validated for simultaneous determination of Sofosbuvir, Velpatasvir and Voxilaprevir in tablet dosage forms. The drugs were separated on Discovery C18 (150 x 4.6 mm, 5m) column using 0.1% othophosphoric acid and Acetonitrile ratio (60:40%v/v) as the mobile phase at a buffer having pH 2.2. The mobile phase is pump into the column at flow rate of 1ml/min and column oven temperature is maintained at 30°C. The drugs were detected at a wavelength 220nm. The retention time for Sofosbuvir, Velpatasvir and Voxilaprevir were found to be 2.120min, 3.164 min and 3.800min. %RSD. The percentage recovery drugs were found to be in range of 99.08%, 98.97% and 99.29% respectively. The Limit of detection, Limit of quantification were 0.21ppm, 0.62ppm, 0.03ppm, 0.09ppm and 0.27ppm, 0.81ppm respectively. Regression equation of concentration over there peak area were found to be Sofosbuvir was y =28579.x + 23225, Velpatasvire was y = 38719x +11703and of Voxilaprevir was y = 38712.x + 74459, Y is the peak area and X is the concentration of drug. The method is useful in the simultaneous determination of third generation antiviral drugs by RP-HPLC.