IMMUNOASSAYTECHNIQUES: PRINCIPLES, CLASSIFICATION, ADVANCEMENTS, AND APPLICATIONS—A COMPREHENSIVE REVIEW

IMMUNOASSAYTECHNIQUES: PRINCIPLES, CLASSIFICATION, ADVANCEMENTS, AND APPLICATIONS—A COMPREHENSIVE REVIEW

Authors

  • Venkatesh. S RKP College of Pharmacy, Krishnagiri, Tamilnadu-635001
  • L.V.Vigneshwaran RKP College of pharmacy, Krishnagiri, Tamilnadu, India.
  • Mohamed Ishaq Mushrif M.N.L RKP College of pharmacy, Krishnagiri, Tamilnadu, India.
  • Jothi Sree.S RKP College of pharmacy, Krishnagiri, Tamilnadu, India.
  • Abinaya.M RKP College of pharmacy, Krishnagiri, Tamilnadu, India.
  • Vinotha.S RKP College of Pharmacy, Krishnagiri, Tamilnadu, India.

Keywords:

Immunoassay, antigen–antibody interactions, chemiluminescent, fluorescence-based, biosensor-coupled

Abstract

Immunoassay techniques are powerful analytical tools that exploit the high specificity of antigen–antibody interactions for qualitative and quantitative analysis of biological molecules. These techniques play a crucial role in clinical diagnostics, pharmaceutical analysis, biomedical research, food safety, and environmental monitoring. Over the years, immunoassays have evolved from classical radioimmunoassays to highly sensitive chemiluminescent, fluorescence-based, biosensor-coupled, and point-of-care systems. This review provides a comprehensive discussion on the principles, classification, working mechanisms, advantages, limitations, recent advancements, and applications of various immunoassay techniques, supported by updated scientific references.

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Published

2026-01-06

How to Cite

Venkatesh. S, L.V.Vigneshwaran, Mohamed Ishaq Mushrif M.N.L, Jothi Sree.S, Abinaya.M, & Vinotha.S. (2026). IMMUNOASSAYTECHNIQUES: PRINCIPLES, CLASSIFICATION, ADVANCEMENTS, AND APPLICATIONS—A COMPREHENSIVE REVIEW. International Journal of Pharmacy and Analytical Research, 15(1), 1–8. Retrieved from https://ijpar.com/ijpar/article/view/1039