Assay Method for Simultaneous Estimation of Epalrestat and Pregabalin in Pure and its Dosage form by RP-HPLC

Abstract

In the proposed method, a new RP-HPLC method has been developed for simultaneous estimation of Epalrestat and Pregabalin in pure and its dosage form. The present method was a sensitive, precise, and accurate RP-HPLC method for analysis of Epalrestat and Pregabalin. To optimize the mobile phase, various combinations of buffer and organic solvents were used on Std BDS C18 column (4.6 x 150mm,5µm). Then the mobile phase containing a mixture 0.1% OPA: Acetonitrile (52:48) was selected at a flow rate of 1ml/min at a detector wavelength of 240 nm foretention time, baseline stability and minimum noise. The retention times of Epalrestat and pregabalin were found to be 2.930 min and 2.179 min respectively. The limit of detection and quantification of Epalrestat and Pregabalin were found to be 0.20 and 0.62; 0.18 and 0.56 respectively, which indicates the sensitivity of the method. The high percentage recovery indicates that the proposed method is highly accurate. No interfering peaks were found in the chromatogram indicating that excipients used in formulations didn’t interfere with the estimation of drugs by the proposed HPLC method.