Stability Indicating Method Development and Validation for the Estimation of Escitalopram and L-Methylfolate in Bulk and Pharmaceutical Dosage Form by RP -HPLC

Abstract

In the presented  work  the stability  indicating  RP-HPLC  method was developed for the simultaneous estimation of the L-methyl folate and Escitalopram in bulk  and pharmaceutical dosage form .The L methyl folate and Escitalopram  were analysed  through  Std BDS C18 150 x 4.6 mm, 5m.Using the mobile phase  consisting of (0.01N KH₂PO₄ buffer:  Acetonitrile)(50:50,v/v) with a flow rate of 0.9ml/min. The wavelength was selected at 230 nm using uv detection. The L Methyl folate and Escitalopram were eluted at 2.232min and 3.279min respectively. The drug was stressed under alkaline, oxidative, thermal, photolytic degradation were analysed. The developed method was validated as per ICH guidelines The Accuracy, Linearity, Precision, and Robustness were within the acceptance limits .Hence this HPLC method was a stability indicating method can be used for routine stability analysis of the Escitalopram and l methyl folate in Pharmaceutical dosage forms.