Analytical method development and validation of pantaprazole in tablet dosage form by using UV spectroscopic method as per ICH guidelines

Abstract

A simple, accurate, precise and economic spectrophotometric method has been developed for the determination of Pantaprazole in their pharmaceutical dosage form. Pantaprazole showed maximum absorbance at 290 nm with Methanol as solvent. Beer’s law was obeyed in the concentration range 10-60 μg/ml with regression coefficient of 0.999. The concentration of active component were then determined from the calibration curve obtained by measuring the amplitude at 290 nm for Pantaprazole. Accuracy and precision of the developed methods have been tested in addition recovery studies have been carried out in order to confirm their accuracy. The method was validated in terms of linearity, precision, accuracy (100.1-100.13%w/w) and specificity. This method is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.