Preparation and Characterization of Isoconazole Loaded Nanolipidic Carrier Gel for Antifungal action

Abstract

Nanoparticulate systems present potential platform for drug therapy that can improve its performance and rise above its limitations. Among the different investigated nano-systems, lipid nanoparticles keep immense promise in the area of drug delivery.  As its lipid content represented only by solid lipid, low drug payload is the major challenge for applicability owing to internal rearrangement of crystal lattice and then drug expulsion. In order to increase drug loading, the second generation lipid nanoparticles; nanostructured lipid carriers (NLCs) was developed. Isoconazole loaded NLC based gel evaluated for Visual appearance and pH, spreadability, viscosity and drug content, In-vitro drug permeation and stability for the optimized formulation. The present investigation deals with formulation and Characterization of Isoconazole loaded NLC based gel. The ingredient s like lipid (Glyceryl mono stearate), liquid lipid (olive oil), surfactant (tween 80) and soya lecithin were selected for formulation using ethanol as solvent. All the nine formulation prepared were evaluated for particle size, entrapment efficiency, zeta potential. The mean particle size was observed in between range of 121±8.14nm to 342±8.55nm, the least mean particle obtained for   formulation FINLC5. Entrapment efficiency was obtained in between the range of 68±2.88 %to 88±1.74%.The highest entrapment efficiency was found for   FINLC5.  Zeta potential for all nine formulation obtained in between -21.3 to -32.1 mV. A surface study of the Isoconazole loaded NLC s using SEM displayed aggregation and fusion of the particles, which could be attributed to the mechanical stress of ice crystals. The Best formulation of Isoconazole loaded NLC (FINLC5) was converted into nine different Gel preparation using Cold method. This Method incorporates the Poloxamer and Carbopol 934 as gelling agent. The best gel found to be FINLCG5 having spreadibility 5.95gm/s, viscosity 2314 centipoise (cP) and drug content 98.76%. In-vitro drug permeation for the optimized FINLCG5. Showed the best result by releasing Isoconazole 98.35% in 12hr. There was no significant changes in Physical appearance, viscosity and drug content was observed in selected FINLCG5 gel formulation after various time point and temperature condition which refers it as a stable formulation. From above data it was concluded that the Isoconazole loaded NLC based gel FINLCG5 Gel is the best formulation can be considered for animal study.