Isolation of active components derived from tuberous root of Ipomoea digitata.

Abstract

The objective of the present investigation was to isolate the active components present in whole plant of Ipomoea digitata. . The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), methanol was found to  be more active among them. The preliminary phytochemical results revealed that flavonoids and amino acids as active constituents in methanolic extract of Ipomoea   digitata.Themethanolic extract of Ipomoea digitata.  was undergone column chromatography with different solvent fractions. Hence, two compounds were isolated from methanolic extract of Ipomoea digitata.  with the compound 1 designated as compound 1 (110 mg)the solvent system(hexane :ethyl acetate80:20), and fractions 42 -50 (eluted with ethyl acetate: Methanol 75:25) gave a solid designated as compound 2 (135mg) compound 2 Rf value was 0.19 using to  solvent  system    (ethyl  acetate:  Methanol  90:10).  The  structures of  the  two  isolated  compounds were characterized  by  using  FT-IR,  NMR  and  Mass  spectrophotometric methods.  Thus,  the  compound  1  was characterized as 3, 4, 5 – Trihydroxy – 6 – (c methoxy carbonyl methyl) – tetrahydro – 2H – Pyran – 2 Carboxylic acid and its molecular formula is C 8H14  O8., and compound 2 was characterized as Tetrahydro 4, 5-dimethoxy-6 (Methoxy methyl)-3 (tetrahydro – 3 – 4, 5, - trimethoxy – 6 – methyl – 2H – Pyran 2 – 2 – y loxy)  –  Pyran-2  Carboxylic  acid  and  its  molecular  formula  C 19   H34   O11.    Therefore,  further  biological investigations need to be carried out isolated compounds present in this plant.