Method development and validation of amoxicillin in bulk and pharmaceutical dosage form by UV spectroscopy
DOI:
https://doi.org/10.61096/ijpar.v8.iss1.2019.153-161Keywords:
Amoxicillin, 0.1N HCL, Method development, Validation, UV spectroscopic method.Abstract
A simple, sensitive, specific, rapid, precise and accurate UV spectrophotometric method has been developed and validated for estimation of Amoxicillin in pure drug and dosage form, using 0.1N HCL as solvent .The absorption maxima was found to be 230nm. The method was validated in terms of Linearity, Precision, Accuracy, Repeatability, Sandell’s sensitivity according to ICH[Q2(R1)] guidelines. Linear response was observed in the range of 1-5µg/ml with a correlation co-efficient of 0.998.The %RSD for Precision was found to be NMT 2 which indicates the method was precise. The %Recovery was found to be in the range of 99.3-99.8% for amoxicillin which indicates a good accuracy of the method. The percentage recovery for the marketed formulation was found to be 99.37%, which indicates good recovery.Commercial capsule formulation was successfully analyzed using the developed method and the proposed method was applicable to routine analysis for determination of Amoxicillin in capsule dosage form.
