Validation of UV spectrophotometric method for the analysis of capsaicin in ethanol
DOI:
https://doi.org/10.61096/ijpar.v11.iss2.2022.115-120Keywords:
Capsaicin, method validation, UV/visible spectrophotometer, linear-calibration curve, ICH-guidelines.Abstract
Method validation of gift sample capsaicin was carried out in calibrated double beam UV/visible spectrophotometer (Shimazdu UV-01800) using ethanol as diluent phase. Though, Capsaicin showed maximum absorbance peaks at 227nm and 280 nm, a 280nm wavelength was considered as absorbance peak as it was very strong at this wavelength. The correlation coefficient (R2) and linear regression equation were found to be 0.9924 and Y= 0.0155x -0.0338 respectively in linear- calibration curve. Further, this method was validated in terms of Linearity, Accuracy, Precision, Limit of Detection (LOD), Limit of Quantification (LOQ), repeatability and robustness as per ICH guidelines.