Stability indicating development and validation for simultaneous estimation of ciprofloxacin and fluocinolone RP-HPLC method

Abstract

The objective of study was to develop simple, Accurate, precise method for the simultaneous estimation of the Ciprofloxacin and Fluocinolone in Tablet dosage form. Chromatogram was run through Std Kromasil 250 x 4.6 mm, 5m. Mobile phase containing Buffer Ortho phosphoric acid: Acetonitrile taken in the ratio 45:55 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% Ortho phosphoric acid buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 230 nm. Retention time of Ciprofloxacin and Fluocinolone were found to be 2.149 min and 3.513. %RSD of the Ciprofloxacin and Fluocinolone were and found to be 0.6 and 0.5 respectively. %Recovery was obtained as 99.36% and 99.71% for Ciprofloxacin and Fluocinolone respectively. LOD, LOQ values obtained from regression equations of Ciprofloxacin and Fluocinolone were 0.13, 0.39 and 0.02, 0.06 respectively. Regression equation of Ciprofloxacin is y = 26832.x + 26137, and of y = 53678.x + 5169 Fluocinolone.