A new HPLC method development and validation for simultaneous determination of acetaminophen and codeiene phosphate in bulk and dosage forms

Abstract

In current research an easy, accurate, reliable, specific and economical HPLC approach is established to analyze acetaminophen and codeine phosphate. Quantification and separation of acetaminophen and codeine phosphate is done with C18 water's column using mobile phase -0.1M, pH 3.5 NaH2PO4: acetonitrile (50; 50). Linearity for acetaminophen was 150µg/ml -450µg/ml and for codeine    phosphate was 30µg/ml -90µg/ml. LOD was 2.770µg/ml for acetaminophen and 0.842 µg/ml for codeine phosphate. Precision on was lesser  than 2.0% and accuracy was near to 100%.The objective of this research deals with optimization of method conditions to estimate acetaminophen and codeine phosphate also includes method validation, specificity, linearity, precision, accuracy and robustness. This developed procedure can therefore employed in pharmaceutical formulations for intent of quality control.