RP-HPLC method development and validation for simultaneous estimation of nebivolol and valsartan in pharmaceutical dosage forms

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Nebivolol Valsartan in Tablet dosage form. Chromatogram was through Std Discovery C18 150 * 4.6 mm, 5 and Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260.0 nm. Retention time of Nebivolol and Valsartan were found to 2.227 min and 3.126 %RSD of the Nebivolol and Valsartan were and found to be 0.6 and 0.4 respectively. %Recovery was obtained as 99.94% and 100.02% for Nebivolol and Valsartan respectively. LOD, LOQ values obtained from regression equations of Nebivolol and Valsartan were 0.05, 0.16 and 0.18, 0.53 respectively. Regression equation of Nebivolol is y=10542.x+470.4, y = 13049x+16927 of Valsartan. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.