Efficient and economic spectroscopic method for determination and validation of atenolol

Abstract

The aim of this work was focused on to determine and validate an efficient, economic, accurate, simple spectroscopic method for the determination of atenolol in bulk drugs. The spectroscopic method was performed using a double beam UV-visible spectrophotometer (1700, Pharmaspace, Shimadzu, Kyoto, Japan). Dilutions of different concentration were prepared using solvents of different pH (2.5, 4.5, 6.5) and absorbance of the solution was determined. Validation was performed by maximum absorbance for each buffer and λmax was 224.30, 224.50 and 225.00 for pH 2.5, 4.5, and 6.5 respectively. Accuracy, interday and intraday precision, recovery and linearity studies were performed. Limit of detection (0.133, 0.133 and 0.2938 for pH 2.5, 4.5 and 6.5 respectively), and limit of quantification (0.404,  0.404  and  0.8905  for  pH  2.5,  4.5,  and  6.5  respectively).    Hence,  this  method  can  be recommended for the estimation and validation of atenolol in bulk drugs and in pharmaceutical dosage form.