Formulation and evaluation of levofloxacin niosomes

Abstract

The delivery of drugs by “vesicular drug delivery system” such as nano-niosomes provides several important advantages over conventional drug therapy. This study reports the development of a highly stable niosomal nanostructure based on Span 60, span 80/cholesterol, chitosan system and its potential application for oral delivery of Levofloxacin. Levofloxacin loaded niosomes were prepared by reversed-phase evaporation and Chitosan coating was performed by incubation of niosomal suspensions with Chitosan solution. The prepared niosomes were characterized for entrapment efficiency (EE), in vitro drug release, drug release kinetics, particle size, zeta potential, surface morphology, anti microbial activity and stability study. Highest entrapment efficiency was observed in LNF-3 79.11. The study of drug release kinetics showed that formulations The formulation LNF-3 released 99.03% of drug for the prolonged period of time (12 hours) than other formulations. governed by higuchi kinetic model (R²=9792). Particle size and zeta potential of the LNF-3 formulation was found to be 339.40 nm with unimodal distribution (PDI 0.160), +23.1mV with spherical morphology. The in-vitro antimicrobial efficiency of optimized noisome formulation enhanced 2-fold by compared with Levofloxacin alone, revealed that niosomes formulations have stronger inhibitory activity. The optimized noisome formulation showed excellent stability in for over 90 days at 40°C.