A New Validated RP-HPLC Method for Determination of Antiemetic Drug (Granisetron) In Bulk and Pharmaceutical Dosage Form

Authors

  • B. Jyothi
  • H. Parameshwar Omega College of Pharmacy, Edulabad, Ghatkesar, Osmani University, Hyderabad, Telangana, India.
  • A.V. Jithan
  • K. Sindoora
  • C.A. Sri Ranjani

Keywords:

RP-HPLC, Granisetron, Determination, Validation parameters

Abstract

RP-HPLC technique has been created and approved for the examination of Granisetron API. Promote the proposed RP-HPLC technique has magnificent affectability, exactness and reproducibility to develop a precise, linear, specific & suitable stability indicating RP-HPLC method for analysis of Granisetron, different chromatographic conditions were applied Isocratic elution is simple, requires only one pump & flat baseline separation for easy and reproducible results.. RP-HPLC various columns are Symmetry ODS (C18) RP Column, 250 mm x 4.6 mm, 5µm column was preferred because using this column peak shape, resolution and absorbance were good. Mobile Phase &diluents for preparation of various samples were finalized after studying the solubility of API in different solvents of our disposal (methanol, Acetonitrile, dichloromethane, water, 0.1N NaOH, 0.1NHCl). The drug was found to be Soluble in DMSO (100 mM), Acetonitrile and methanol. Sparingly Soluble in ethanol and Water. Using these solvents with appropriate composition newer methods can be developed and validated. Recognition wavelength was chosen in the wake of checking the standard arrangement of medication more than 200 to 400nm. From the U.V range of Granisetron it is apparent that a large portion of the HPLC works can be refined in the wavelength scope of 206 nm helpfully. Further, a stream rate of 1 ml/min and an infusion volume of 10µl were observed to be the best examination. The outcome demonstrates the created strategy is amazingly, one more appropriate technique for test and steadiness related contamination thinks about which can help in the examination of Granisetron in various definitions.

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Published

2022-05-21