UV spectrophotometric method development and validation of Cilnidipine API and marketed pharmaceutical dosage form
DOI:
https://doi.org/10.61096/ijpar.v9.iss2.2020.62-67Keywords:
Uv spectroscopy, Cilnidipine, ICH guidelines.Abstract
The present research work to develop and validate a simple, accurate, precise and economic method for estimation of Cinidipine in bulk and tablet dosage form by using UV spectroscopy. Ethanol was used as solvent for Cilnidipine. The UV spectrum of Cilnidipine showed λmax at 240nm and obeyed Beer-Lambert’s law in the concentration range of 2-14μg/ml. The method was shown linear with line equation y=0.062x-0.012, with correlation coefficient r2 =0.988. The accuracy values for Cilnidipine ranged from 97.8% to 100.06%. The %RSD of inter day precision was 0.52% to 1.92% (%RSD less than 2) and intra day precision was 0.672% to less than 2%.The system suitability studies in both pure and marketed formulation was within limits ( %RSD Less than 2). The LOD & LOQ range was 0.0638μg/ml & 0.193μg/ml. Hence the proposed method is applicable for quantitative determination of bulk drug and pharmaceutical dosage form for routine analysis.