Development and validation of RP-HPLC method for simultaneous estimation of paracetamol and chlorzoxazone in bulk form

Authors

  • Hajera N. Khan Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Mahajan Swarali Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Chopde Asha Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Mohammad Zameeruddin Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Vishvanath B. Bharkad Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.

DOI:

https://doi.org/10.61096/ijpar.v6.iss3.2017.550-554

Keywords:

RP-HPLC, Paracetamol, Chlorzoxazone, Validation

Abstract

High performance liquid chromatography (HPLC) method was developed and validated for the analysis of Paracetamol and Chlorzoxazone. Chromatographic separation achieved isocretically on C -18 column Zorbax Eclipse XDB- C18 (4.6~250mm~5µ). Utilizing a mobile phase Acetonitrile: Water in the ratio 50:50v/v. with a flow  rate  of  1.5ml/min.  UV  detection  was  carried  o ut  at  274nm.  The  retention time  of  Paracetamol  and Chlorzoxazone <10 min respectively. The developed method was validated in terms of recovery, precision,,rggedness, robustness, linearity as per ICH guidelines. This study aimed at developing and validating an HPLC method.

Downloads

Published

2022-10-11

How to Cite

Hajera N. Khan, Mahajan Swarali, Chopde Asha, Mohammad Zameeruddin, & Vishvanath B. Bharkad. (2022). Development and validation of RP-HPLC method for simultaneous estimation of paracetamol and chlorzoxazone in bulk form. IJPAR JOURNAL, 6(3), 550–554. https://doi.org/10.61096/ijpar.v6.iss3.2017.550-554

Issue

Vol. 6 No. 3 (2017): 2017 Volume - 6 Issue - 3

Section

Articles