HPTLC fingerprint profile of selected anti-diabetic plants monitored with reference to flavonoids

Abstract

The main objective of the present study was to evaluate the phytoconstituents and finger print profile in the various extracts of two important anti-diabetic species namely Terminalia catappa and Cressa cretica. The plant species were extracted with different solvents such as petroleum ether,  chloroform, ethyl acetate and  methanol in the increasing order of polarity. Preliminary phytochemical screening of the various extracts of these two species revealed the presence of flavonoids, phenols, tannins and saponins in the methanolic extract. HPTLC methods for the separation of the active constituents in extracts have been developed. HPTLC finger print for different extracts revealed the presence of peaks with Rf  values in the range of 0.01 to 0.99. The bands of purple and light yellowish orange colour revealed the presence of flavonoids and phenolic compounds. Routine quality control of these species could be carried out using this hptlc method and it serves in qualitative analysis, quantitative estimation and was appropriate for standardization of the extract.