New spectrophotometric method development for the estimation of vildagliptin in bulk & Pharmaceutical dosage form

Abstract

A new simple, specific, rapid, reproducible and cost effective spectrophotometric method using Schiff’s base reaction for the quantitative estimation of Vildagliptin in bulk and pharmaceutical dosage form. The developed UV spectrophotometric method for the quantitative estimation of Vildagliptin is based on the measurement of absorption at maximum wavelength 250nm using Vanillin Sulphuric acid as a reagent, ethanol as a solvent & methanol as a diluent. The stock solution of Vildagliptin was prepared and subsequently Vanillin sulphuric acid reagent was added. Finally, methanol was used to make up the volume in order to obtain the standard curve. The standard solution of Vildagliptin showed absorption maxima at 250nm. The drug obeyed Beer lambert’s law in the concentration range of 100-1000µg/ml with λmaxat 250nm. The developed method can be adopted in routine analysis of Vildagliptin in bulk and tablet dosage form.