HPTLC finger print profile of n-hexane extract of Rauwolfia tetraphylla Linn

Abstract

The objective of the present study is to evaluate the phytochemical composition and HPTLC finger print profile of
medicinally useful plant R. tetraphylla L. (Apocyanaceae) leaf n-hexane extract. The CAMAG HPTLC system was used
for the finger print profiling using the mobile phase toluene: ethyl acetate: acetic acid (55:45:1 v/v). The profile showed
that the leaf extract of R. tetraphylla exhibited several peaks with different Rf values when visualized. At 254nm and 366
nm .At 254 nm a total 14 peaks were observed and at 366nm 10 peaks were observed. The HPTLC profile of R.
tetraphylla leaf n-hexane extract is used to identify the number of chemical components and their concentration; this in
turn helps to identify the chemical constituents in medicinal products and also in identification of adulterants in medicinal
products mainly herbal medicine.